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67.200 - Edible oils and fats. Oilseeds

ICS 67.200 Details

Edible oils and fats. Oilseeds

Speiseole. Speisefette. Olsaaten

Huiles et corps gras comestibles. Graines oleagineuses

Jedilna olja in maščobe. Oljnice

General Information

Parent
67 - FOOD TECHNOLOGY
Sub-Items
67.200.10 - Animal and vegetable fats and oils
67.200.20 - Oilseeds

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Standardization Organization
Technical Committee
Directive
Mandate
50
ISO
ISO 18363-3:2024(Main)
Animal and vegetable fats and oils — Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS — Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol

This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis. This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

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SIST
SIST EN ISO 20122:2024(Main)
Vegetable oils - Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID) analysis - Method for low limit of quantification (ISO 20122:2024)
EN ISO 20122:2024

This International standard method specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled HPLC-GC-FID. This standard is not intended to be applied to other matrices.
The method can be used for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.5. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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EN ISO 20122:2024(Main)
Vegetable oils - Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID) analysis - Method for low limit of quantification (ISO 20122:2024)
SIST EN ISO 20122:2024

This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices.
The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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ISO
ISO 20122:2024(Main)
Vegetable oils — Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID) analysis — Method for low limit of quantification

This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices. The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH). According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg. In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS. An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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ISO
ISO/TS 16465:2024(Main)
Animal and vegetable fats and oils — Determination of phthalates in vegetable oils

This document specifies two methods for the quantitative determination of phthalates in vegetable oils by gas chromatography-mass spectrometry (GC-MS): — Part A for the determination of di-2-ethylhexyl phthalate (DEHP). — Part B for the determination of eight phthalates: dimethyl phthalate (DMP), diethyl phthalate (DEP), di-isobutyl phthalate (DIBP), dibutyl phthalate (DBP), benzylbutyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), di-isononyl phthalate (DINP), di-isodecyl phthalate (DIDP). Both methods are applicable for all vegetable oils, including crude, refined and virgin.

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SIST
SIST EN ISO 3657:2023(Main)
Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2023)
EN ISO 3657:2023

This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids.
The method is applicable to refined and crude vegetable and animal fats.
If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately.
The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.

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EN ISO 3657:2023(Main)
Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2023)
SIST EN ISO 3657:2023

This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids.
The method is applicable to refined and crude vegetable and animal fats.
If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately.
The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.

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ISO
ISO 3657:2023(Main)
Animal and vegetable fats and oils — Determination of saponification value

This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids. The method is applicable to refined and crude vegetable and animal fats. If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately. The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.

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SIST
SIST EN ISO 734:2023(Main)
Oilseed meals - Determination of oil content - Extraction method with hexane (or light petroleum) (ISO 734:2023)
EN ISO 734:2023

ISO 734:2015 specifies a method for the determination of the hexane extract (or light-petroleum extract), called "oil content", of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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ISO
ISO 24363:2023(Main)
Determination of fatty acid methyl esters (cis and trans) and squalene in olive oil and other vegetable oils by gas chromatography

This document specifies the determination of the fatty acid methyl esters (FAME) and squalene in olive oil and other vegetable oils by gas chromatography (GC). This document is applicable to the determination of FAME from C12 to C24, including saturated, cis- and trans-monounsaturated, cis- and trans-polyunsaturated FAME and squalene.

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CEN
EN ISO 734:2023(Main)
Oilseed meals - Determination of oil content - Extraction method with hexane (or light petroleum) (ISO 734:2023)
SIST EN ISO 734:2023

This document specifies a method for the determination of the hexane extract (or light-petroleum extract), called “oil content”, of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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ISO
ISO 734:2023(Main)
Oilseed meals — Determination of oil content — Extraction method with hexane (or light petroleum)

This document specifies a method for the determination of the hexane extract (or light-petroleum extract), called “oil content”, of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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CEN
EN 14111:2022(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of iodine value
SIST EN 14111:2022

This document specifies a titrimetric method for the determination of iodine value in Fatty Acid Methyl Esters, hereinafter referred as FAME.
The precision statement of this test method was determined in a Round Robin exercise with iodine values in the range 111 g iodine/100 g to 129 g iodine/100 g.
The test method is also applicable for lower iodine values; however, the precision statement is not established for iodine values below 111 g iodine/100 g.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.

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SIST
SIST EN ISO 12872:2023(Main)
Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2022)
EN ISO 12872:2022

ISO 12872:2010 specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate content in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).

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ISO
ISO 23942:2022(Main)
Determination of hydroxytyrosol and tyrosol content in extra virgin olive oils — Reverse phase high performance liquid chromatography (RP-HPLC) method

This document specifies a method for the quantitative determination of hydroxytyrosol and tyrosol content in extra virgin olive oils using reverse phase high performance liquid chromatography (RP-HPLC) with spectrophotometric detection. The method is also applicable to all other olive oils of a different commercial category.

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CEN
EN ISO 12872:2022(Main)
Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2022)
SIST EN ISO 12872:2023

This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).
NOTE       This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].

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ISO
ISO 12872:2022(Main)
Olive oils and olive-pomace oils — Determination of the 2-glyceryl monopalmitate content

This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C). NOTE This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].

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SIST
SIST EN 14111:2022(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of iodine value
EN 14111:2022

This document specifies a titrimetric method for the determination of iodine value in Fatty Acid Methyl Esters, hereinafter referred as FAME.
The precision statement of this test method was determined in a Round Robin exercise with iodine values in the range 111 g iodine/100 g to 129 g iodine/100 g.
The test method is also applicable for lower iodine values, however, the precision statement is not established for iodine values below 111 g iodine/100 g.
WARNING — The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.

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CEN
EN ISO 18363-1:2021(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)
SIST EN ISO 18363-1:2022

ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.

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EN ISO 18363-3:2021(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2017)
SIST EN ISO 18363-3:2022

ISO 18363-3:2017 specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
ISO 18363-3:2017 is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

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ISO
ISO/TS 12788:2022(Main)
Rapeseed — Determination of glucosinolate content — Spectrometric method for total glucosinolates by glucose release

This document specifies a method for the determination of the content of the total glucosinolates in rapeseeds (colza) using visible spectrometry to determine the glucose released from glucosinolates by hydrolysis.

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EN ISO 18363-4:2021(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 4: Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS (ISO 18363-4:2021)
SIST EN ISO 18363-4:2021

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.
This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

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SIST
SIST EN ISO 18363-3:2022(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2017)
EN ISO 18363-3:2021

This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

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SIST
SIST EN ISO 18363-1:2022(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)
EN ISO 18363-1:2021

This part of ISO 18363 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
This part of ISO 18363 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.

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CEN
EN ISO 6321:2021(Main)
Animal and vegetable fats and oils - Determination of melting point in open capillary tubes - Slip point (ISO 6321:2021)
SIST EN ISO 6321:2021

This document specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as fats hereinafter).
—    Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and which do not exhibit pronounced polymorphism.
—    Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and is the method to be used for fats whose polymorphic behaviour is unknown.
For the determination of the slip melting point of palm oil samples the method given in Annex A shall be used.
NOTE 1   If applied to fats with pronounced polymorphism, method A will give different and less satisfactory results than method B.
NOTE 2   Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.

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SIST EN ISO 18363-4:2021(Main)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 4: Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS (ISO 18363-4:2021)
EN ISO 18363-4:2021

This part of ISO 18363 describes a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis.
This method is applicable to solid and liquid fats and oils. This part of ISO 18363 can also apply to animal fats and used frying oils and fats, but a validation study must be undertaken before the analysis of these matrices.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this international standard.

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SIST
SIST-TS CEN ISO/TS 22115:2021(Main)
Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method) (ISO/TS 22115:2021)
CEN ISO/TS 22115:2021

This method is suitable for the semi-quantitative analysis of oils, fats and oil/fat-related samples
(deodistillates).
Screening of oils, fats and oil/fat-related samples to obtain main (e.g. TAGs) and minor component (e.g.
sterols, sterol esters, tocopherols, squalene, wax esters, fatty alcohols, and glycerol) information in one
single analysis. For a truly quantitative analysis of pre-identified compound classes specific methods
are more appropriate.
Beside the (semi-)quantitative determination of the oil/fat composition mentioned above, the method
can also be used as a useful qualitative screening tool for the relative comparison of sample
compositions.

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ISO
ISO 18363-4:2021(Main)
Animal and vegetable fats and oils — Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS — Part 4: Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure. This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg. Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

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CEN
EN 14104:2021(Main)
Fat and oil derivates - Fatty acid methyl ester (FAME) - Determination of acid value
SIST EN 14104:2021

This document specifies a titrimetric method for the determination of acid value in light coloured Fatty Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.
NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (V/V)" are used to represent respectively the mass fraction and the volume fraction.
NOTE 2 For oils and fats the determination of acid value is specified in EN ISO 660 [1].
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.

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SIST
SIST EN ISO 6321:2021(Main)
Animal and vegetable fats and oils - Determination of melting point in open capillary tubes - Slip point (ISO 6321:2021)
EN ISO 6321:2021

This document specifies two methods for the determination of the melting point in open capillary
tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as
fats hereinafter).
— Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and
which do not exhibit pronounced polymorphism.
— Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and
is the method to be used for fats whose polymorphic behaviour is unknown.
For the determination of the slip melting point of palm oil samples the method given in Annex A shall be
used.
NOTE 1 If applied to fats with pronounced polymorphism, method A will give different and less satisfactory
results than method B.
NOTE 2 Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing
appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.

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CEN
CEN ISO/TS 22115:2021(Main)
Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method) (ISO/TS 22115:2021)
SIST-TS CEN ISO/TS 22115:2021

This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates).
It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate.
The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.

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CEN
EN 14105:2020(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of free and total glycerol and mono-, di-, triglyceride contents
SIST EN 14105:2021

This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results.
Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks.
NOTE 1 For the purposes of this document, the term "% (m/m)" is used to represent the mass fraction.
NOTE 2 Under the common EN 14105 GC conditions squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.

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ISO
ISO/TS 22115:2021(Main)
Animal and vegetable fats and oils — Separation of lipid classes by capillary gas chromatography (fingerprint method)

This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates). It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate. The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.

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ISO
ISO 6321:2021(Main)
Animal and vegetable fats and oils — Determination of melting point in open capillary tubes — Slip point

This document specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as fats hereinafter). — Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and which do not exhibit pronounced polymorphism. — Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and is the method to be used for fats whose polymorphic behaviour is unknown. For the determination of the slip melting point of palm oil samples the method given in Annex A shall be used. NOTE 1 If applied to fats with pronounced polymorphism, method A will give different and less satisfactory results than method B. NOTE 2 Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.

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CEN
EN 14112:2020(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of oxidation stability (accelerated oxidation test)
SIST EN 14112:2021

This document specifies a method for the determination of the oxidation stability of fatty acid methyl esters (FAME) at 110 °C, by means of measuring the induction period up to 48 h.
For induction periods higher than 8,5 h the precision is not covered by the precision statement of this method.
NOTE 1 EN 15751 [1] describes a similar test method for oxidation stability determination of pure fatty acid methyl esters and of blends of FAME with petroleum-based diesel containing 2 % (V/V) of FAME at minimum.
NOTE 2 Limited studies on EN 15751 with EHN (2-ethyl hexyl nitrate) on FAME blends indicated that the stability is reduced to an extent which is within the reproducibility of the test method. It is likely that the oxidation stability of pure FAMEs is also reduced in the presence of EHN when EN 14112 is used for testing.
NOTE 3 For the purposes of this document, the term ''% (V/V)'' is used to represent the volume fraction.

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CEN
EN ISO 660:2020(Main)
Animal and vegetable fats and oils - Determination of acid value and acidity (ISO 660:2020)
SIST EN ISO 660:2020

This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as "fats". The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally.
This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes.
Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document.

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ISO
ISO 771:2021(Main)
Oilseed meals — Determination of moisture and volatile matter content

This document specifies a method for the determination of the moisture and volatile matter content of oilseed meals obtained by the extraction of oil from oilseeds by pressure and/or solvent.

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CEN
EN ISO 7540:2020(Main)
Spices and condiments - Ground sweet and hot paprika (Capsicum annuum L. and Capsicum frutescens L.) - Specifications (ISO 7540:2020)
SIST EN ISO 7540:2020

This document specifies requirements for ground sweet and hot paprika (Capsicum annuum L. and Capsicum frutescens L.).
Recommendations relating to storage and transport conditions are given in Annex A. A list of terms used in different countries for paprika is given in Annex B.
This document does not apply to ground chillies and other species of capsicums.
NOTE    Specifications for ground chillies and capsicums are given in ISO 972.

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SIST
SIST EN 14104:2021(Main)
Fat and oil derivates - Fatty acid methyl ester (FAME) - Determination of acid value
EN 14104:2021

This document specifies a titrimetric method for the determination of acid value in light coloured Fatty Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.
NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (V/V)" are used to represent respectively the mass fraction and the volume fraction.
NOTE 2 For oils and fats the determination of acid value is specified in EN ISO 660 [1].
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.

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SIST
SIST EN 14105:2021(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of free and total glycerol and mono-, di-, triglyceride contents
EN 14105:2020

The purpose of this European Standard is to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME) intended for addition to mineral oils. The total glycerol content is then calculated from the obtained results.
Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks.
NOTE  For the purposes of this European Standard, the term "% (m/m)" is used to represent respectively the mass fraction.
WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use. It is the responsibility of the user of this standard to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.

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SIST
SIST EN 14112:2021(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of oxidation stability (accelerated oxidation test)
EN 14112:2020

This European Standard specifies a method for the determination of the oxidation stability of fatty acid methyl esters (FAME) at 110 °C, by means of measuring the induction period up to 48 h.
NOTE 1   EN 15751 [1] describes a similar test method for oxidation stability determination of pure fatty acid methyl esters and of blends of FAME with petroleum-based diesel containing 2 % (V/V) of FAME at minimum.
NOTE 2   The precision statement of this test method was determined in a Round Robin exercise with induction periods up to 8,5 h, thus covering the limit value in EN 14214. Results from precision studies on EN 15751 indicate that the precision statement is valid for induction periods up to 48 h but not for higher values.
NOTE 3   Limited studies on EN 15751 with EHN (2-ethyl hexyl nitrate) on FAME blends indicated that the stability is reduced to an extent which is within the reproducibility of the test method. It is likely that the oxidation stability of pure FAMEs is also reduced in the presence of EHN when EN 14112 is used for testing.

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SIST
SIST EN ISO 660:2020(Main)
Animal and vegetable fats and oils - Determination of acid value and acidity (ISO 660:2020)
EN ISO 660:2020

This document specifies three methods (two titrimetric and one potentiometric) for the determination
of acidity in animal and vegetable fats and oils, hereinafter referred to as “fats”. The acidity is expressed
preferably as acid value or, alternatively, as acidity calculated conventionally.
This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty
acids or technical fatty acids. It does not apply to waxes.
Since the methods are completely non-specific, they do not apply to differentiating between mineral
acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that
are present.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this
document.

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CEN
EN ISO 665:2020(Main)
Oilseeds - Determination of moisture and volatile matter content (ISO 665:2020)
SIST EN ISO 665:2020

This document specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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CEN
EN 14103:2020(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of ester and linolenic acid methyl ester contents
SIST EN 14103:2020

The purpose of this document is to describe a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m).
This method is suitable for FAME which contains methyl esters between C6 and C24.
NOTE 1   For the purposes of this document, the terms "% (m/m)" and "% (v/v)" are used to represent respectively the mass and volume fractions.
NOTE 2   This method was elaborated for FAME samples from usual raw material. For FAME sample from unidentified raw material, a solution of the test sample should be prepared without any internal standard addition, in order to verify the absence of natural nonadecanoic acid methyl ester.
NOTE 3   The distribution off fatty acid methyl esters is given in Annex C.
WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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SIST
SIST EN ISO 665:2020(Main)
Oilseeds - Determination of moisture and volatile matter content (ISO 665:2020)
EN ISO 665:2020

EN-ISO 665 specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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CEN
EN ISO 17059:2019(Main)
Oilseeds - Extraction of oil and preparation of methyl esters of triglyceride fatty acids for analysis by gas chromatography (Rapid method) (ISO 17059:2019)
SIST EN ISO 17059:2019

This document specifies a rapid method for extraction of oil and for preparation of the methyl esters of fatty acids. The methyl esters thus obtained can be used for gas chromatography.
This document is applicable to the following oilseeds: rape and mustard with low erucic acid content (< 2 %), sunflower, soya beans, linseed.
NOTE    Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid content by approximately a mass fraction of 1 %. This difference was observed in Reference [6] and could be due to the partial extraction of the oil from the sample (yield around 70 %). High content of erucic acid in triglycerides could increase their solubility in hexane because of the lipophilic effect of the carbon long-chain (C22). However, as this effect was not checked on a large set of high erucic rapeseed samples, it is not appropriate to apply a correction factor to the erucic acid content when analysing high erucic acid rapeseed.

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ISO
ISO 660:2020(Main)
Animal and vegetable fats and oils — Determination of acid value and acidity

This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as "fats". The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally. This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes. Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present. Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document.

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ISO
ISO 665:2020(Main)
Oilseeds — Determination of moisture and volatile matter content

This document specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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ISO
ISO 3475:2020(Main)
Essential oil of aniseed (Pimpinella anisum L.)

This document specifies certain characteristics of the essential oil of aniseed (Pimpinella anisum L.), with a view to facilitating the assessment of its quality.

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SIST EN 14103:2020(Main)
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of ester and linolenic acid methyl ester contents
EN 14103:2020

The purpose of this document is to describe a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m).
This method is suitable for FAME which contains methyl esters between C6 and C24.
NOTE 1   For the purposes of this document, the terms "% (m/m)" and "% (v/v)" are used to represent respectively the mass and volume fractions.
NOTE 2   This method was elaborated for FAME samples from usual raw material. For FAME sample from unidentified raw material, a solution of the test sample should be prepared without any internal standard addition, in order to verify the absence of natural nonadecanoic acid methyl ester.
NOTE 3   The distribution off fatty acid methyl esters is given in Annex C.
WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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