Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor

Graines de colza — Détermination de la teneur en chlorophylle — Méthode spectrométrique — Amendement 1: Préparation de la courbe d’étalonnage pour déterminer le facteur k

General Information

Status
Published
Publication Date
06-Apr-2025
Current Stage
6060 - International Standard published
Start Date
07-Apr-2025
Due Date
23-May-2025
Completion Date
07-Apr-2025

Relations

Effective Date
17-Jun-2023

Overview

ISO 10519:2015/Amd 1:2025 is an important amendment to the ISO 10519:2015 standard, which specifies the spectrometric method for determining chlorophyll content in rapeseed. This amendment introduces detailed procedures for the preparation of the calibration curve used to determine the k factor-a crucial constant impacting the accuracy of chlorophyll measurement by spectrophotometry.

The k factor depends on the path length and spectral bandwidth of the spectrophotometer and is essential for converting absorbance readings into precise chlorophyll concentrations. This amendment addresses variability in spectrophotometer bandwidth and provides guidance to ensure consistent and accurate testing.


Key Topics

  • Chlorophyll content analysis in rapeseed
    Precise quantification of chlorophyll levels is vital for quality control in rapeseed processing and related agricultural products.

  • Spectrometric method calibration
    The amendment details the preparation of calibration curves using chlorophyll A standard solutions, allowing laboratories to determine the k factor specific to their instrument settings.

  • k factor determination
    The k factor is influenced by the spectrophotometer’s physical slit width, optical dispersion, and spectral bandwidth settings. This document outlines how to establish this constant to improve measurement accuracy.

  • Spectrophotometer bandwidth variability
    Bandwidth variations can significantly affect absorbance resolution and accuracy. The amendment emphasizes the need to set and maintain a constant bandwidth during testing.

  • Standardized reagents and equipment
    Guidelines specify the use of high-purity chlorophyll A, diethyl ether solvent, appropriate volumetric flasks, micropipettes, and spectrometers capable of measurements between 600 nm and 700 nm with specified bandwidths.


Applications

  • Agricultural quality control
    Enables seed producers and laboratories to accurately measure chlorophyll content in rapeseed, ensuring compliance with quality standards.

  • Oilseed processing industry
    Chlorophyll content influences oil color and quality; precise measurement supports refining processes and product consistency.

  • Research and development
    Facilitates reproducible chlorophyll quantification in experimental studies involving rapeseed and related oilseed crops.

  • Standardization of optical measurement instruments
    Assists laboratories in calibrating spectrophotometers, thereby harmonizing testing results across different equipment and facilities.


Related Standards

  • ISO 10519:2015
    The base standard for determining chlorophyll content in rapeseed by spectrometric methods.

  • ISO/IEC Directives, Part 1 and Part 2
    Define procedures and editorial rules for preparing and maintaining ISO standards.

  • CEN/TC 307 standards
    European Committee for Standardization documents related to oilseeds, vegetable and animal fats, oils, and their by-products, including sampling and analysis methods.


This amendment to ISO 10519 enhances the reliability of spectral analysis for chlorophyll in rapeseed by providing a standardized way to calibrate and determine the critical k factor. Implementing these calibration steps ensures that spectrometric chlorophyll determinations are accurate, reproducible, and compliant with international best practices, benefiting the agricultural and food industries worldwide.

Keywords: ISO 10519 amendment, rapeseed chlorophyll determination, spectrometric method, k factor calibration, chlorophyll A standard, spectrophotometer bandwidth, oilseed quality control, chlorophyll measurement standard.

Standard

ISO 10519:2015/Amd 1:2025 - Rapeseed — Determination of chlorophyll content — Spectrometric method — Amendment 1: Preparation of the calibration curve to determine the k factor Released:7. 04. 2025

English language
2 pages
sale 15% off
Preview
sale 15% off
Preview
Standard

ISO 10519:2015/Amd 1:2025 - Graines de colza — Détermination de la teneur en chlorophylle — Méthode spectrométrique — Amendement 1: Préparation de la courbe d’étalonnage pour déterminer le facteur k Released:7. 04. 2025

French language
3 pages
sale 15% off
Preview
sale 15% off
Preview

Frequently Asked Questions

ISO 10519:2015/Amd 1:2025 is a standard published by the International Organization for Standardization (ISO). Its full title is "Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor". This standard covers: Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor

Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor

ISO 10519:2015/Amd 1:2025 is classified under the following ICS (International Classification for Standards) categories: 67.200.20 - Oilseeds. The ICS classification helps identify the subject area and facilitates finding related standards.

ISO 10519:2015/Amd 1:2025 has the following relationships with other standards: It is inter standard links to ISO 10519:2015. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.

ISO 10519:2015/Amd 1:2025 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


International
Standard
ISO 10519
Third edition
Rapeseed — Determination
2015-09-15
of chlorophyll content —
Spectrometric method
AMENDMENT 1
2025-04
AMENDMENT 1: Preparation of the
calibration curve to determine the
k factor
Graines de colza — Détermination de la teneur en chlorophylle —
Méthode spectrométrique
AMENDEMENT 1: Préparation de la courbe d’étalonnage pour
déterminer le facteur k
Reference number
ISO 10519:2015/Amd.1:2025(en) © ISO 2025

ISO 10519:2015/Amd.1:2025(en)
© ISO 2025
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 10519:2015/Amd.1:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 2, Oleaginous seeds and fruits and oilseed meals, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
...


Norme
internationale
ISO 10519
Troisième édition
Graines de colza — Détermination
2015-09-15
de la teneur en chlorophylle —
Méthode spectrométrique
AMENDEMENT 1
2025-04
AMENDEMENT 1: Préparation de la
courbe d’étalonnage pour déterminer
le facteur k
Rapeseed — Determination of chlorophyll content —
Spectrometric method
AMENDMENT 1: Preparation of the calibration curve to
determine the k factor
Numéro de référence
ISO 10519:2015/Amd.1:2025(fr) © ISO 2025

ISO 10519:2015/Amd.1:2025(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2025
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: +41 22 749 01 11
E-mail: copyright@iso.org
Web: www.iso.org
Publié en Suisse
ii
ISO 10519:2015/Amd.1:2025(fr)
Avant-propos
L’ISO (Organisation internationale de normalisation) est une fédération mondiale d’organismes nationaux
de normalisation (comités membres de l’ISO). L’élaboration des Normes internationales est en général
confiée aux comités techniques de l’ISO. Chaque comité membre intéressé par une étude a le droit de faire
partie du comité technique créé à cet effet. Les organisations internationales, gouvernementales et non
gouvernementales, en liaison avec l’ISO participent également aux travaux. L’ISO collabore étroitement avec
la Commission électrotechnique internationale (IEC) en ce qui concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier, de prendre note des différents
critères d’approbation requis pour les différents types de documents ISO. Le présent document a
été rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir
www.iso.org/directives).
L’ISO attire l’attention sur le fait que la mise en application du présent document peut entraîner l’utilisation
d’un ou de plusieurs brevets. L’ISO ne prend pas position quant à la preuve, à la validité et à l’applicabilité de
tout droit de brevet revendiqué à cet égard. À la date de publication du présent document, l’ISO n’avait pas
reçu notification qu’un ou plusieurs brevets pouvaient être nécessaires à sa mise en application. Toutefois,
il y a lieu d’avertir les responsables de la mise en application du présent document que des informations
plus récentes sont susceptibles de figurer dans la base de données de brevets, disponible à l’adresse
www.iso.org/brevets. L’ISO ne saurait être tenue pour responsable de ne pas avoir identifié tout ou partie de
tels droits de propriété.
Les appellations commerciales éventuellement mentionnées dans le présent document sont données pour
information, par souci de commodité, à l’intention des utilisateurs et ne sauraient constituer un engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l’ISO liés à l’évaluation de la conformité, ou pour toute information au sujet de l’adhésion de
l’ISO aux principes de l’Organisation mondiale du commerce (OMC) concernant les obstacles techniques au
commerce (OTC), voir www.iso.org/avant-propos.
Le présent document a été élaboré par le comité technique ISO/TC 34, Produits alimentaires, sous-comité SC 2,
Graines et fruits oléagineux et farines de graines oléagineuses, en collaboration avec le comité technique CEN/
TC 307, Oléagineux, corps gras d’origines végétale et animale et leurs co-produits - Méthodes d’échantillonnage
et d’analyse, du Comité européen de normalisation (CEN) conformément à l’Accord de coopération technique
entre l’ISO et le CEN (Accord de Vienne).
Il convient que l’utilisateur adresse tout retour d’information ou toute question concernant le présent
document à l’organisme national de normalisation de son pays. Une liste exhaustive desdits organismes se
trouve à l’adresse www.iso.org/fr
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.

Loading comments...