EN ISO 18363-1:2021

SLOVENSKI STANDARD
01-februar-2022
Živalske in rastlinske maščobe ter olja - Določevanje maščobno-kislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 1. del: Metoda z uporabo hitre
alkalne transesterifikacije in meritve 3-MCPD ter diferencialne meritve glicidola
(ISO 18363-1:2015)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and differential measurement for
glycidol (ISO 18363-1:2015)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 1: Verfahren mittels schneller
alkalischer Umesterung und Messung für 3 MCPD und Differenzmessung für Glycidol
(ISO 18363 1:2015)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 1: Méthode par transestérification alcaline rapide et mesure pour le
3-MCPD et par mesure différentielle pour le glycidol (ISO 18363-1:2015)
Ta slovenski standard je istoveten z: EN ISO 18363-1:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18363-1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2021
EUROPÄISCHE NORM
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of fatty-
acid-bound chloropropanediols (MCPDs) and glycidol by
GC/MS - Part 1: Method using fast alkaline
transesterification and measurement for 3-MCPD and
differential measurement for glycidol (ISO 18363-1:2015)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des esters de chloropropanediols von fettsäuregebundenen Chlorpropandiol (MCPD)
(MCPD) et d'acides gras et des esters de glycidol et und Glycidol mittels GC/MS - Teil 1: Verfahren mittels
d'acides gras par CPG/SM - Partie 1: Méthode par schneller alkalischer Umesterung und Messung für 3
transestérification alcaline rapide et mesure pour le 3- MCPD und Differenzmessung für Glycidol (ISO 18363
MCPD et par mesure différentielle pour le glycidol (ISO 1:2015)
18363-1:2015)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

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Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18363-1:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 18363-1:2015 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18363-
1:2021 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18363-1:2015 has been approved by CEN as EN ISO 18363-1:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 18363-1
First edition
2015-08-15
Animal and vegetable fats and oils —
Determination of fatty-acid-bound
chloropropanediols (MCPDs) and
glycidol by GC/MS —
Part 1:
Method using fast alkaline
transesterification and measurement
for 3-MCPD and differential
measurement for glycidol
Corps gras d’origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d’acides gras et des esters de
glycidol et d’acides gras —
Partie 1: Méthode par transestérification alcaline rapide et
mesure pour le chloro-3 propane-1,2-diol (3-MCPD) et par mesure
différentielle pour le glycidol
Reference number
ISO 18363-1:2015(E)
©
ISO 2015
ISO 18363-1:2015(E)
© ISO 2015, Published in Switzerland
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ii © ISO 2015 – All rights reserved

ISO 18363-1:2015(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
5.1 General . 3
5.2 Standard and reference compounds . 3
5.3 Solvents . 3
5.4 Other reagents . 3
6 Apparatus . 4
7 Sample . 4
7.1 Sampling . 4
7.2 Preparation of the test sample . 4
8 Procedure. 5
8.1 Spiking with surrogate standard and homogenization . 5
8.2 Ester cleavage and glycidol transformation . 5
8.3 Matrix clean up . 5
8.4 Derivatization . 5
8.5 Gas chromatography/mass spectrometry references . 6
9 Expression of results . 6
10 Precision . 7
10.1 Interlaboratory test. 7
10.2 Repeatability . 8
10.3 Reproducibility . 8
11 Test report . 8
Annex A (informative) Results of an collaborative trial . 9
Bibliography .11
ISO 18363-1:2015(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
differ
...