67.200.10 - Animal and vegetable fats and oils
ICS 67.200.10 Details
Animal and vegetable fats and oils
Tierische und pflanzliche Fette und Ole
Corps gras d'origines animale et végétale
Rastlinske in živalske maščobe in olja
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This document specifies the determination of composition of triacylglycerols and the determination of the composition and content of diacylglycerols by capillary gas chromatography in vegetable oils with a lauric acid content below 1 %. Applying certain technological processing 1,2-diacylglycerols (1,2-DAGs) are transformed to the more stable isomeric 1,3-diacylglycerols (1,3-DAGs) due to acidic catalysed reaction. During storage, the speed and amount of this rearrangement depends on the acidity of the oil. The transformation normally reaches an equilibrium between the two isomeric forms. The relative amount of 1,2-DAGs is related to oil freshness or to a possible technological treatment. Therefore, it is possible to use the ratio of 1,2-DAGs to 1,3-DAGs as a quality criterion for vegetable fats and oils. The triacylglycerols profile is of potential interest for the fingerprint of each vegetable oil and may help the detection of certain types of adulteration, such as the addition of high oleic sunflower oil or palm olein in olive oil. NOTE This document is based on Reference [ REF Reference_ref_3 \r \h 3 08D0C9EA79F9BACE118C8200AA004BA90B0200000008000000100000005200650066006500720065006E00630065005F007200650066005F0033000000 ].
- Standard13 pagesEnglish languagesale 15% off
This document specifies a procedure for the direct determination of the content of the soap building elements Calcium (Ca), Magnesium (Mg), Sodium (Na) and Potassium (K) as well as Phosphorus (P) in fatty acid methyl esters (FAME) by ICP OES.
The concentrations of each component or the combinations of some to which this method is applicable are given in Table 1.
Table 1 - Scope ranges for each element
Element Scope range
mg/kg
Ca 0,3 - 5,4
Mg 0,3 - 4,6
Na 0,4 - 5,0
K 0,6 - 5,3
P 1,0 - 5,0
Ca + Mg 0,5 - 9,4
Na + K 1,0 - 9,9
Ca + Mg + Na + K 1,4 - 19,3
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
NOTE For the purposes of this document, the term "% (V/V)" is used to represent the volume fraction, φ, of a material.
- Standard11 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte.
This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
- Standard30 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a reference method for the determination of the iodine value (commonly known in the industry as IV) of animal and vegetable fats and oils, hereinafter referred to as fats.
Annex B describes a method for the calculation of the IV from fatty acid compositional data. This method is not applicable to fish oils. Furthermore, cold-pressed, crude and unrefined vegetable oils as well as (partially) hydrogenated oils can give different results by the two methods. The calculated IV is affected by impurities and thermal degradation products.
NOTE The method in Annex B is based upon the AOCS Official method Cd 1c-85[10].
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte.
This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
- Standard30 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte. This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
- Standard21 pagesEnglish languagesale 15% off
- Standard22 pagesFrench languagesale 15% off
This document specifies a reference method for the determination of the iodine value (commonly known in the industry as IV) of animal and vegetable fats and oils, hereinafter referred to as fats.
Annex B describes a method for the calculation of the IV from fatty acid compositional data. This method is not applicable to fish oils. Furthermore, cold-pressed, crude and unrefined vegetable oils as well as (partially) hydrogenated oils can give different results by the two methods. The calculated IV is affected by impurities and thermal degradation products.
NOTE The method in Annex B is based upon the AOCS Official method Cd 1c-85[10].
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the direct determination of the content of the soap building elements Calcium (Ca), Magnesium (Mg), Sodium (Na) and Potassium (K) as well as Phosphorus (P) in fatty acid methyl esters (FAME) by ICP OES.
The concentrations of each component or the combinations of some to which this method is applicable are given in Table 1.
Table 1 - Scope ranges for each element
Element Scope range
mg/kg
Ca 0,3 - 5,4
Mg 0,3 - 4,6
Na 0,4 - 5,0
K 0,6 - 5,3
P 1,0 - 5,0
Ca + Mg 0,5 - 9,4
Na + K 1,0 - 9,9
Ca + Mg + Na + K 1,4 - 19,3
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
NOTE For the purposes of this document, the term "% (V/V)" is used to represent the volume fraction, φ, of a material.
- Standard11 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a reference method for the determination of the iodine value (commonly known in the industry as IV) of animal and vegetable fats and oils, hereinafter referred to as fats. Annex B describes a method for the calculation of the IV from fatty acid compositional data. This method is not applicable to fish oils. Furthermore, cold-pressed, crude and unrefined vegetable oils as well as (partially) hydrogenated oils can give different results by the two methods. The calculated IV is affected by impurities and thermal degradation products. NOTE The method in Annex B is based upon the AOCS Official method Cd 1c-85[10].
- Standard11 pagesEnglish languagesale 15% off
- Standard12 pagesFrench languagesale 15% off
This document specifies a method for the determination of the aflatoxins B1, B2, G1 and G2 in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization. The limits of quantification for the aflatoxins B1, B2, G1 and G2, and for the sum of aflatoxins B1, B2, G1 and G2, are 1 μg/kg, 0,25 μg/kg, 0,5 μg/kg, 0,25 μg/kg and 1 μg/kg, respectively. The validation was carried out over the following concentration ranges: — aflatoxin B1 = 1 μg/kg to 20 μg/kg; — total aflatoxins = 2 μg/kg to 52 μg/kg.
- Standard19 pagesEnglish languagesale 15% off
This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results.
Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks.
NOTE 1 For the purposes of this document, the term "% (m/m)" is used to represent the mass fraction.
NOTE 2 Under the common EN 14105 GC conditions, squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.
- Standard27 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the analysis of phenolic antioxidants by high-performance liquid chromatography (HPLC). It is applicable to quantifying the following synthetic phenolic compounds added to animal and vegetable fats, oils and shortenings as antioxidants, at concentrations normally added to oils: — propyl gallate (PG); — octyl gallate (OG); — dodecyl gallate (also called “lauryl gallate (LG)”); — 2,4,5-trihydroxybutyrophenone (THBP); — tert-butylhydroquinone (TBHQ); — nordihydroguaiaretic acid (NDGA); — 2- and 3-tert-butyl-4-hydroxyanisole (BHA); — 2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHT Alcohol or Ionox-100); — 2,6-di-tert-butyl-4-hydroxytoluene (BHT). A method for determining the absence of an antioxidant, or the maximum trace amount, within the limits of the analysis, is given in Annex B. The issue of canolol, a naturally occurring substance in rapeseed, interfering with the analysis is addressed in Annex C.
- Draft14 pagesEnglish languagesale 15% off
- Draft14 pagesEnglish languagesale 15% off
This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results.
Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks.
NOTE 1 For the purposes of this document, the term "% (m/m)" is used to represent the mass fraction.
NOTE 2 Under the common EN 14105 GC conditions, squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.
- Standard27 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
- Standard26 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard26 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
- Standard26 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard26 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis. This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
- Standard17 pagesEnglish languagesale 15% off
- Standard18 pagesFrench languagesale 15% off
This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices.
The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).
- Standard50 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard50 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices.
The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).
- Standard50 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard50 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices. The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH). According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg. In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS. An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).
- Standard42 pagesEnglish languagesale 15% off
- Standard42 pagesEnglish languagesale 15% off
- Standard43 pagesFrench languagesale 15% off
- Standard43 pagesFrench languagesale 15% off
- Standard43 pagesFrench languagesale 15% off
This document specifies two methods for the quantitative determination of phthalates in vegetable oils by gas chromatography-mass spectrometry (GC-MS): — Part A for the determination of di-2-ethylhexyl phthalate (DEHP). — Part B for the determination of eight phthalates: dimethyl phthalate (DMP), diethyl phthalate (DEP), di-isobutyl phthalate (DIBP), dibutyl phthalate (DBP), benzylbutyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), di-isononyl phthalate (DINP), di-isodecyl phthalate (DIDP). Both methods are applicable for all vegetable oils, including crude, refined and virgin.
- Technical specification25 pagesEnglish languagesale 15% off
This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids.
The method is applicable to refined and crude vegetable and animal fats.
If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately.
The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.
- Standard17 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids.
The method is applicable to refined and crude vegetable and animal fats.
If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately.
The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.
- Standard17 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids. The method is applicable to refined and crude vegetable and animal fats. If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately. The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.
- Standard10 pagesEnglish languagesale 15% off
This document specifies the determination of the fatty acid methyl esters (FAME) and squalene in olive oil and other vegetable oils by gas chromatography (GC). This document is applicable to the determination of FAME from C12 to C24, including saturated, cis- and trans-monounsaturated, cis- and trans-polyunsaturated FAME and squalene.
- Standard22 pagesEnglish languagesale 15% off
This document specifies a titrimetric method for the determination of iodine value in Fatty Acid Methyl Esters, hereinafter referred as FAME.
The precision statement of this test method was determined in a Round Robin exercise with iodine values in the range 111 g iodine/100 g to 129 g iodine/100 g.
The test method is also applicable for lower iodine values; however, the precision statement is not established for iodine values below 111 g iodine/100 g.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.
- Standard10 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).
NOTE This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].
- Standard23 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the quantitative determination of hydroxytyrosol and tyrosol content in extra virgin olive oils using reverse phase high performance liquid chromatography (RP-HPLC) with spectrophotometric detection. The method is also applicable to all other olive oils of a different commercial category.
- Standard13 pagesEnglish languagesale 15% off
- Standard13 pagesFrench languagesale 15% off
This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).
NOTE This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].
- Standard23 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C). NOTE This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].
- Standard15 pagesEnglish languagesale 15% off
- Standard15 pagesFrench languagesale 15% off
- Standard15 pagesFrench languagesale 15% off
This document specifies a titrimetric method for the determination of iodine value in Fatty Acid Methyl Esters, hereinafter referred as FAME.
The precision statement of this test method was determined in a Round Robin exercise with iodine values in the range 111 g iodine/100 g to 129 g iodine/100 g.
The test method is also applicable for lower iodine values; however, the precision statement is not established for iodine values below 111 g iodine/100 g.
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.
- Standard10 pagesEnglish languagesale 10% offe-Library read for1 day
ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.
This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.
- Standard32 pagesEnglish languagesale 10% offe-Library read for1 day
ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD.
ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as fats hereinafter).
— Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and which do not exhibit pronounced polymorphism.
— Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and is the method to be used for fats whose polymorphic behaviour is unknown.
For the determination of the slip melting point of palm oil samples the method given in Annex A shall be used.
NOTE 1 If applied to fats with pronounced polymorphism, method A will give different and less satisfactory results than method B.
NOTE 2 Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.
This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.
Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.
- Standard32 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates).
It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate.
The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.
- Technical specification29 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure. This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg. Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.
- Standard23 pagesEnglish languagesale 15% off
- Standard24 pagesFrench languagesale 15% off
This document specifies a titrimetric method for the determination of acid value in light coloured Fatty Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.
NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (V/V)" are used to represent respectively the mass fraction and the volume fraction.
NOTE 2 For oils and fats the determination of acid value is specified in EN ISO 660 [1].
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.
- Standard10 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as fats hereinafter).
— Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and which do not exhibit pronounced polymorphism.
— Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and is the method to be used for fats whose polymorphic behaviour is unknown.
For the determination of the slip melting point of palm oil samples the method given in Annex A shall be used.
NOTE 1 If applied to fats with pronounced polymorphism, method A will give different and less satisfactory results than method B.
NOTE 2 Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates).
It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate.
The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.
- Technical specification29 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates). It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate. The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.
- Technical specification22 pagesEnglish languagesale 15% off
- Technical specification23 pagesFrench languagesale 15% off
This document specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip melting point, of animal and vegetable fats and oils (referred to as fats hereinafter). — Method A is only applicable to animal and vegetable fats which are solid at ambient temperature and which do not exhibit pronounced polymorphism. — Method B is applicable to all animal and vegetable fats which are solid at ambient temperature and is the method to be used for fats whose polymorphic behaviour is unknown. For the determination of the slip melting point of palm oil samples the method given in Annex A shall be used. NOTE 1 If applied to fats with pronounced polymorphism, method A will give different and less satisfactory results than method B. NOTE 2 Fats which exhibit pronounced polymorphism are principally cocoa butter and fats containing appreciable quantities of 2-unsaturated, 1,3-saturated triacylglycerols.
- Standard11 pagesEnglish languagesale 15% off
- Standard12 pagesFrench languagesale 15% off
This document specifies a method for the determination of the oxidation stability of fatty acid methyl esters (FAME) at 110 °C, by means of measuring the induction period up to 48 h.
For induction periods higher than 8,5 h the precision is not covered by the precision statement of this method.
NOTE 1 EN 15751 [1] describes a similar test method for oxidation stability determination of pure fatty acid methyl esters and of blends of FAME with petroleum-based diesel containing 2 % (V/V) of FAME at minimum.
NOTE 2 Limited studies on EN 15751 with EHN (2-ethyl hexyl nitrate) on FAME blends indicated that the stability is reduced to an extent which is within the reproducibility of the test method. It is likely that the oxidation stability of pure FAMEs is also reduced in the presence of EHN when EN 14112 is used for testing.
NOTE 3 For the purposes of this document, the term ''% (V/V)'' is used to represent the volume fraction.
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as "fats". The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally.
This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes.
Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document.
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies requirements for ground sweet and hot paprika (Capsicum annuum L. and Capsicum frutescens L.).
Recommendations relating to storage and transport conditions are given in Annex A. A list of terms used in different countries for paprika is given in Annex B.
This document does not apply to ground chillies and other species of capsicums.
NOTE Specifications for ground chillies and capsicums are given in ISO 972.
- Standard14 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a titrimetric method for the determination of acid value in light coloured Fatty Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.
NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (V/V)" are used to represent respectively the mass fraction and the volume fraction.
NOTE 2 For oils and fats the determination of acid value is specified in EN ISO 660 [1].
WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to the application of the document, and to determine the applicability of any other restrictions for this purpose.
- Standard10 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the determination of the oxidation stability of fatty acid methyl esters (FAME) at 110 °C, by means of measuring the induction period up to 48 h.
For induction periods higher than 8,5 h the precision is not covered by the precision statement of this method.
NOTE 1 EN 15751 [1] describes a similar test method for oxidation stability determination of pure fatty acid methyl esters and of blends of FAME with petroleum-based diesel containing 2 % (V/V) of FAME at minimum.
NOTE 2 Limited studies on EN 15751 with EHN (2-ethyl hexyl nitrate) on FAME blends indicated that the stability is reduced to an extent which is within the reproducibility of the test method. It is likely that the oxidation stability of pure FAMEs is also reduced in the presence of EHN when EN 14112 is used for testing.
NOTE 3 For the purposes of this document, the term ''% (V/V)'' is used to represent the volume fraction.
- Standard18 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as "fats". The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally.
This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes.
Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document.
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
The purpose of this document is to describe a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m).
This method is suitable for FAME which contains methyl esters between C6 and C24.
NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (v/v)" are used to represent respectively the mass and volume fractions.
NOTE 2 This method was elaborated for FAME samples from usual raw material. For FAME sample from unidentified raw material, a solution of the test sample should be prepared without any internal standard addition, in order to verify the absence of natural nonadecanoic acid methyl ester.
NOTE 3 The distribution off fatty acid methyl esters is given in Annex C.
WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as "fats". The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally. This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes. Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present. Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document.
- Standard12 pagesEnglish languagesale 15% off
- Standard12 pagesFrench languagesale 15% off