EN ISO 20122:2024

SLOVENSKI STANDARD
01-junij-2024
Rastlinska olja - Določevanje nasičenih ogljikovodikov mineralnih olj (MOAH) in
aromatskih ogljikovodikov mineralnih olj (MOAH) z on-line sklopljeno analizo s
tekočinsko kromatografijo visoke ločljivosti in plinsko kromatografijo v povezavi s
plamenskim ionizacijskim detektorjem (HPLC-GC-FID) - Metoda za nizko mejo
določljivosti (ISO 20122:2024)
Vegetable oils - Determination of mineral oil saturated hydrocarbons (MOSH) and
mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid
chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID)
analysis - Method for low limit of quantification (ISO 20122:2024)
Pflanzliche Öle - Bestimmung von gesättigten Mineralölkohlenwasserstoffen (MOSH)
und aromatischen Kohlenwasserstoffen (MOAH) mit online gekoppelter HPLC-GC-FID-
Analyse - Verfahren für die niedrige Bestimmungsgrenze (ISO 20122:2024)
Huiles végétales - Dosage des hydrocarbures saturés d’huile minérale (MOSH) et des
hydrocarbures aromatiques d’huile minérale (MOAH) par analyse par chromatographie
en phase liquide haute performance et chromatographie en phase gazeuse couplées à
un détecteur à ionisation de flamme (CLHP-CG-FID) en ligne - Méthode pour une faible
limite de quantification (ISO 20122:2024)
Ta slovenski standard je istoveten z: EN ISO 20122:2024
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 20122
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2024
EUROPÄISCHE NORM
ICS 67.200.10
English Version
Vegetable oils - Determination of mineral oil saturated
hydrocarbons (MOSH) and mineral oil aromatic
hydrocarbons (MOAH) with online-coupled high
performance liquid chromatography-gas chromatography-
flame ionization detection (HPLC-GC-FID) analysis -
Method for low limit of quantification (ISO 20122:2024)
Huiles végétales - Dosage des hydrocarbures saturés Pflanzliche Öle - Bestimmung von gesättigten
d'huile minérale (MOSH) et des hydrocarbures Mineralölkohlenwasserstoffen (MOSH) und
aromatiques d'huile minérale (MOAH) par analyse par aromatischen Kohlenwasserstoffen (MOAH) mit online
chromatographie en phase liquide haute performance gekoppelter HPLC-GC-FID-Analyse - Verfahren für die
et chromatographie en phase gazeuse couplées à un niedrige Bestimmungsgrenze (ISO 20122:2024)
détecteur à ionisation de flamme (CLHP-CG-FID) en
ligne - Méthode pour une faible limite de quantification
(ISO 20122:2024)
This European Standard was approved by CEN on 12 January 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20122:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 20122:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2024, and conflicting national standards shall
be withdrawn at the latest by October 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 20122:2024 has been approved by CEN as EN ISO 20122:2024 without any modification.

International
Standard
ISO 20122
First edition
Vegetable oils — Determination of
2024-04
mineral oil saturated hydrocarbons
(MOSH) and mineral oil aromatic
hydrocarbons (MOAH) with
online-coupled high performance
liquid chromatography-gas
chromatography-flame ionization
detection (HPLC-GC-FID) analysis
— Method for low limit of
quantification
Reference number
ISO 20122:2024(en) © ISO 2024
ISO 20122:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
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Published in Switzerland
ii
ISO 20122:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 6
7 Sample . 7
7.1 Sampling .7
7.2 Preparation of the final sample for liquid and solid fats .7
8 Procedures . 8
8.1 General .8
8.2 Hexane/ethanol distribution for removal of interfering substances .8
8.3 Saponification . .8
8.4 Removal of biogenic n-alkanes with aluminium oxide for determination of the MOSH
fraction .9
8.5 Clean-up before epoxidation to separate polar substances .9
8.6 Ethanolic epoxidation of the MOAH fraction to oxidize unsaturated non-aromatic
compounds .9
8.7 HPLC-GC separation .10
8.7.1 HPLC conditions .10
8.7.2 GC configuration .10
8.7.3 Solvent vapour exit configuration .11
8.7.4 Peak identification .11
8.7.5 System suitability test . 12
8.8 Blank run . 13
8.9 Quality control . 13
9 Result of the determination .13
9.1 Testing the chromatograms for sufficient epoxidation and other relevant parameters. 13
9.2 Calculation .14
10 Precision of the method .15
10.1 Repeatability limit . 15
10.2 Reproducibility limit . 15
11 Test report .15
Annex A (informative) Graphics and chromatograms.
...