EN 14104:2021

SLOVENSKI STANDARD
01-marec-2021
Nadomešča:
SIST EN 14104:2003
Derivati maščob in olj - Metil estri maščobnih kislin (FAME) - Določevanje
kislinskega števila
Fat and oil derivates - Fatty acid methyl ester (FAME) - Determination of acid value
Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester
(FAME) - Bestimmung der Säurezahl
Produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) -
Détermination de l'indice d'acide
Ta slovenski standard je istoveten z: EN 14104:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 14104
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2021
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN 14104:2003
English Version
Fat and oil derivates - Fatty acid methyl ester (FAME) -
Determination of acid value
Produits dérivés des corps gras - Esters méthyliques Erzeugnisse aus pflanzlichen und tierischen Fetten und
d'acides gras (EMAG) - Détermination de l'indice Ölen - Fettsäure-Methylester (FAME) - Bestimmung
d'acide der Säurezahl
This European Standard was approved by CEN on 6 December 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 14104:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
1 Scope . 4
2 Normative references . 4
3 Terms and definitions . 4
4 Principle . 4
5 Reagents . 5
6 Apparatus . 6
7 Sampling . 6
8 Preparation of the test sample . 6
9 Procedure . 7
9.1 Test portion . 7
9.2 Determination . 7
10 Calculation . 7
11 Precision . 7
11.1 Interlaboratory test . 7
11.2 Repeatability . 7
11.3 Reproducibility . 7
12 Test report . 8
Annex A (informative) Results of an interlaboratory trial . 9
Bibliography . 10

European foreword
This document (EN 14104:2021) has been prepared by Technical Committee CEN/TC 307 “Oilseeds,
vegetable and animal fats and oils and their by-products - Methods of sampling and analysis”, the
secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2021, and conflicting national standards shall be
withdrawn at the latest by July 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 14104:2003.
In comparison with the previous edition, the following technical modifications have been made:
a) Clause 5 “Reagents” revised for clarification;
b) automated titration system applying optical detection added as alternative;
c) document revised editorially.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
1 Scope
This document specifies a titrimetric method for the determination of acid value in light coloured Fatty
Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.
NOTE 1 For the purposes of this document, the terms “% (m/m)” and “% (V/V)” are used to represent
respectively the mass fraction and the volume fraction.
NOTE 2 For oils and fats the determination of acid value is specified in EN ISO 660 [1].
WARNING — The use of this document can involve hazardous materials, operations and equipment.
This document does not purport to address all of the safety problems associated with its use. It is the
responsibility of users of this document to take appropriate measures to ensure the safety and health of
personnel prior to the application of the document, and to determine the applicability of any other
restrictions for this purpose.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 661, Animal and vegetable fats and oils - Preparation of test sample (ISO 661)
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
• IEC Electropedia: available at http://www.electropedia.org/
• ISO Online browsing platform: available at https:/
...