SIST EN ISO 13366-3:1998

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Mleko - Ugotavljanje števila somatskih celic - 3. del: Fluorooptoelektronska metoda (ISO 13366-3:1997)Milch - Zählung somatischer Zellen - Teil 3: Fluoreszenzoptoelektronisches Verfahren (ISO 13366-3:1997)Lait - Dénombrement des cellules somatiques - Partie 3: Méthode fluoro-opto-électronique (ISO 13366-3:1997)Milk - Enumeration of somatic cells - Part 3: Fluoro-opto-electronic method (ISO 13366-3:1997)67.100.10SURL]YRGLMilk and processed milk products07.100.30Mikrobiologija živilFood microbiologyICS:Ta slovenski standard je istoveten z:EN ISO 13366-3:1997SIST EN ISO 13366-3:1998en01-avgust-1998SIST EN ISO 13366-3:1998SLOVENSKI
STANDARD



SIST EN ISO 13366-3:1998



SIST EN ISO 13366-3:1998



SIST EN ISO 13366-3:1998



INTERNATIONAL STANDARD IS0 13366-3 First edition 1997-06-I 5 Milk - Enumeration of somatic cells - Part 3: Fluoro-opto-electronic method Lait - Dknombrement des cellules somatiques - Partie 3: Mkthode fluoro-opto-klectronique Reference number IS0 13366-3: 1997(E) SIST EN ISO 13366-3:1998



IS0 13366-3: 1997(E) Foreword IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies (IS0 member bodies). The work of preparing International Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non- governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard IS0 13366 was prepared by Technical Committee ISOmC 34, Agricultural food products, Subcommittee SC 5, Milk and milk products, in collaboration with the International Dairy Federation (IDF) and AOAC INTERNATIONAL, and will also be published by these organiz- ations. IS0 13366 consists of the following parts, under the general title Milk - Enumeration of soma tic cells: - Part I: Microscopic method - Par? 2: Electronic particle counter method - Part 3: Fluoro-opto-electronic method Annexes A to D of this part of IS0 13366 are for information only. 0 IS0 1997 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case postale 56 l CH-1211 Geneve 20 l Switzerland Internet central @I isocs.iso.ch x.400 c=ch; a=400net; p=iso; o=isocs; s=central Printed in Switzerland ii SIST EN ISO 13366-3:1998



~~ INTERNATIONAL STANDARD @ IS0 IS0 13366-3: 1997(E) Milk - Enumeration of somatic cells - Part 3: Fluoro-opto-electronic method WARNING - The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use. 1 Scope This part of IS0 13366 specifies a method for counting somatic cells in both raw and chemically preserved milk, using a fluoro-opto-electronic counting instrumentI). NOTE - Counting of cells in unpreserved samples within the first 24 h after milking could give unreliable results with older instruments (e.g. Fossomatic 90 and 215). 2 Definition For the purposes of this part of IS0 13366, the following definition applies. 2.1 somatic cells: Those cells that have a minimum intensity of fluorescence due to the staining of DNA in their nuclei. 3 Principle Mixing of the milk to be examined with a buffer and stain solution. Transference of the mixture in the form of a thin film to a rotating disc, serving as an object plane for a microscope. Each stained cell observed by the microscope produces an electrical pulse that is amplified and recorded. Direct reading of the number of somatic cells in thousands per millilitre. 4 Reagents WARNING - Ethidium bromide is toxic. The preparation and application of the basic and working solutions shall be carried out in a fume cupboard. Use gloves for protection. 1) The Fossomatic counting instrument (250, 300 or 360) supplied by Foss Electric, Hillerod, Denmark is an example of suitable equipment available commercially. This information is given for the convenience of users of this part of IS0 13366 and does not constitute an endorsement by IS0 of the eq
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