kSIST FprEN ISO 25202:2026
(Main)Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/FDIS 25202:2026)
- Abstract
This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
- Status
- Not Published
- Public Enquiry End Date
- 27-Feb-2025
- Technical Committee
- IUSN - Leather
- Current Stage
- 5020 - Formal vote (FV) (Adopted Project)
- Start Date
- 23-Jul-2026
- Due Date
- 10-Sep-2026
- Completion Date
- 24-Jul-2026
kSIST FprEN ISO 25202:2026 is a SIST publication for leather chemical analysis. It specifies a liquid chromatography (LC) method for determining free and released glutaraldehyde in leather, so laboratories can measure this analyte in leather materials and finished leather goods.
What does kSIST FprEN ISO 25202:2026 specify?
kSIST FprEN ISO 25202:2026 specifies a test method for glutaraldehyde in leather and uses LC as the measurement technique. The method is aimed at chemical analysis work on leather, where a repeatable extraction, derivatization, and chromatographic measurement are needed.
The document is organized into 10 clauses and one informative annex. That structure takes the user from scope and references through specimen preparation, testing, calculation, and reporting.
| Annex | What it covers |
|---|---|
| Annex A | Informative LC conditions |
What are the key requirements of kSIST FprEN ISO 25202:2026?
Clause 1 limits the method to the determination of free and released glutaraldehyde in leather by LC. In practice, that tells the laboratory what analyte the result applies to and what analytical route to use.
Clause 2 requires the use of ISO 2418, ISO 3696, and ISO 4044 where they are cited in the method. This matters because sampling, water quality, and preparation of chemical test samples all affect the reliability of the result.
Clause 4 describes a two-stage measurement principle: the leather extract is treated with 2,4-dinitrophenylhydrazine (DNPH), then the derivatives are separated by reversed-phase LC and detected at 355 ± 5 nm. In practice, the lab must derivatize the aldehydes before analysis and run a UV-detected chromatographic method.
Clause 7 requires sampling in accordance with ISO 2418 and cutting the leather in accordance with ISO 4044. If sampling from finished products such as shoes or garments is not possible in that way, the test report has to include the sampling details. That keeps the result traceable to the actual test piece.
Clause 8.1 specifies the extraction conditions: about 2 g of leather, 50 ml of detergent solution, 40 ± 2 °C, and 60 ± 2 min. The method also says not to change the leather-to-solution ratio and to complete extraction and analysis within the same working day. This is important because extraction time and ratio directly influence how much glutaraldehyde is recovered.
Clause 8.1.2 and 8.2 set up the derivatization and calibration steps. The sample extract is mixed with acetonitrile and DNPH solution, left to react, filtered, and analysed by LC; calibration solutions are prepared over a range of 0.1 µg/ml to 2.0 µg/ml, which corresponds under the stated conditions to 5 mg/kg to 100 mg/kg in leather. For stronger samples, smaller aliquots are used. In practice, the user must match the sample size to the expected concentration.
Clause 9 requires reporting the result to the nearest 0.1 mg/kg and calculating it from the calibration graph and the stated formula. Clause 10 lists the reporting items, including the document reference, sample identification, test date, result, unusual features, deviations from the procedure, and whether the result is based on dry matter. That supports consistent records for quality control, contracts, and audits.
Who uses kSIST FprEN ISO 25202:2026?
kSIST FprEN ISO 25202:2026 is used by laboratories that perform chemical testing of leather. It is also relevant to tanneries, leather product manufacturers, and quality managers who need analytical data on glutaraldehyde in leather materials or in finished items such as shoes and garments.
The standard is also useful for buyers and suppliers who need a common test method for product specifications, acceptance checks, or dispute resolution. Because it gives a defined sampling, extraction, and LC procedure, different parties can compare results more reliably.
Which standards are used with kSIST FprEN ISO 25202:2026?
The document cites ISO 2418 for the position and preparation of leather specimens for testing. It cites ISO 3696 for grade 3 water, which supports the analytical reagent quality needed for the procedure. It cites ISO 4044 for preparation of chemical test samples from leather.
The bibliography also lists ISO 17226-1, a related leather method for formaldehyde content using high-performance liquid chromatography. That helps readers place this glutaraldehyde method within the wider family of leather chemical analysis standards.
What does the kSIST FprEN ISO 25202:2026 document contain?
The document contains the full analytical workflow: scope, references, principle, reagents, apparatus, specimen preparation, sample extraction, DNPH reaction, LC analysis, calculation, precision, and test reporting. Annex A gives example LC conditions, including flow rate, mobile phase program, column type, temperature, injection volume, and UV detection wavelength.
Clause 9 provides the calculation approach for converting the calibration result into glutaraldehyde content in mg/kg. Clause 10 gives the reporting checklist, so the analytical result can be documented in a way that supports review and traceability.
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Frequently Asked Questions
kSIST FprEN ISO 25202:2026 is a draft published by the Slovenian Institute for Standardization (SIST). Its full title is "Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/FDIS 25202:2026)". This standard covers: This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
kSIST FprEN ISO 25202:2026 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs; 71.040.40 - Chemical analysis. The ICS classification helps identify the subject area and facilitates finding related standards.
kSIST FprEN ISO 25202:2026 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN ISO 25202:2025
01-februar-2025
Usnje - Kemijska analiza - Določevanje vsebnosti glutaraldehida (ISO/DIS
25202:2024)
Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS
25202:2024)
Leder - Chemische Analyse - Bestimmung des Glutaraldehydgehalts (ISO/DIS
25202:2024)
Cuir - Analyse chimique - Détermination de la teneur en glutaraldéhyde (ISO/DIS
25202:2024)
Ta slovenski standard je istoveten z: prEN ISO 25202
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.40 Kemijska analiza Chemical analysis
oSIST prEN ISO 25202:2025 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN ISO 25202:2025
oSIST prEN ISO 25202:2025
DRAFT
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
2024-12-11
ICS: 59.140.30
Voting terminates on:
2025-03-05
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference numbers
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
oSIST prEN ISO 25202:2025
DRAFT
ISO/DIS 25202:2024(en)
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
ICS: 59.140.30
Voting terminates on:
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
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NOTIFICATION OF ANY RELEVANT PATENT
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RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
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Website: www.iso.org
Reference numbers
Published in Switzerland ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
ii
oSIST prEN ISO 25202:2025
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Preparation of test specimens . 2
8 Procedure . 3
8.1 Sample processing .3
8.1.1 Extraction .3
8.1.2 Reaction with DNPH .3
8.2 Determination by LC .3
9 Expression of results . 4
9.1 General .4
9.2 Calculation of the glutaraldehyde content .4
9.3 Precision of the test method .4
10 Test report . 4
Annex A (informative) LC conditions . 5
Bibliography . 6
iii
oSIST prEN ISO 25202:2025
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
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patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of
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This document was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 289, Leather, in collaboration with the Chemical Test Commission of the International Union of
Leather Technologists and Chemists Societies (IUC Commission, IULTCS), in accordance with the Agreement
on technical cooperation between ISO and CEN (Vienna Agreement). This method is technically similar to
the Colorimetric Section of the method IUC 19 which was declared an official method at the IULTCS Delegates
meeting on 31st May 2003 i
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