EN 14119:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Preskušanje tekstilij - Ocenjevanje delovanja mikroglivPrüfung von Textilien - Bestimmung der Einwirkung mikroskopischer Pilze (Mikrofungi)Essais sur les textiles - Evaluation de l'action des champignons microscopiquesTesting of textiles - Evaluation of the action of microfungi59.080.30TkanineTextile fabrics07.100.99Drugi standardi v zvezi z mikrobiologijoOther standards related to microbiologyICS:Ta slovenski standard je istoveten z:EN 14119:2003SIST EN 14119:2003en01-december-2003SIST EN 14119:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14119September 2003ICS 07.100.99; 59.080.30English versionTesting of textiles - Evaluation of the action of microfungiEssais sur les textiles - Evaluation de l'action deschampignons microscopiquesPrüfung von Textilien - Bestimmung der Einwirkungmikroskopischer Pilze (Mikrofungi)This European Standard was approved by CEN on 1 August 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14119:2003 ESIST EN 14119:2003

Recommendations for testing.20Annex B (informative)
Information on the test fungi.21SIST EN 14119:2003

0,5 g¾ FeSO4 × 7H2O 0,01 g¾ H2O1 000 mlSIST EN 14119:2003

Add to 1 l of the stock mineral salts solution (7.5.1) 0,1 g ofa non-toxic wetting agent, such asN-methyltauride or polyglycol ether.Sterilize in an autoclave at 121 °C for15 min to 20 min.7.5.3Mineral salts solution with glucose. Add to the mineral salts solution (7.5.1) glucose to obtain aconcentration of (30 ± 1) g/l. Sterilize in an autoclave at 115 °C for 30 min.NOTEIn autoclaves with integrated cooling procedure it is possible to sterilize sugar containing media at 121 °C for15 min.7.5.4 Incomplete agar medium. Add to the mineral salts solution (7.5.1) agar to obtain a concentration of20 g/l. Dissolve the agar by boiling the solutions whilst stirring.Sterilize in an autoclave at 121 °C for 15 to 20 min.7.5.5Complete agar medium. Use the incomplete agar medium (7.5.4) and add glucose to obtain aconcentration of (20 ± 1) g/l.Sterilize in an autoclave at 115 °C for 30 min.Adjust the pH to between 6,0 and 6,5 at 20 °C after sterilisation with a 0,01 mol/l NaOH solution.NOTEIn autoclaves with integrated cooling procedure it is possible to sterilize sugar-containing media at 121 °C for15 min.8
Test specimens8.1 DimensionsThe shape and dimensions of the specimens will depend on tests to be carried out, see the followingprocedures.8.2 Numbers of specimensAt least 4 test specimens shall be taken from the same batch for visual examination.At least 6 test specimens shall be taken from the same batch for the determination of tensile strength.8.3 Batches of specimensFor each sample, each stage of ageing and each test method two batches of specimens should be prepared:¾ batch 0: control specimens, stored under standard temperature and moisture conditions;¾ batch I: specimens used for the biotest.SIST EN 14119:2003

Summary of test proceduresTest proceduresMethodA1A2B1B2Reference10.210.310.410.5medium usedincomplete agarmediumnonecomplete agar mediuminoculummixed sporesuspensionsprayed over testspecimen and agarmediummixed sporesuspensionsprayed on testspecimenmixed sporesuspensionsprayed over testspecimen and agarmediumAspergillus niger resp.Chaetomium globosumspore suspensionincorporated in theagar mediumincubation time2 and 4 weeks2 weeksincubationtemperature29 °Chumidity> 95% r.h.provided by the agarmediumcontrolled humiditywithin the moistchamberprovided by the agar mediumassessmenta) growthb) tensile strengtha) growthb) growth inhibitionc) tensile strengtha) growthb) growth inhibition10.2 Procedure A 1:
Fungus resistance10.2.1 Filling the Petri dishesAfter sterilization pour the incomplete agar medium (5.5) into the sterile Petri dishes to give a depth of 5 mm.Leave to cool and solidify.10.2.2 Dimensions and number of specimensCut specimens of 2.5 cm x 8 cm (length in warp direction for woven textiles or in the direction of cellulosicyarns) and then make test specimens of exactly 20 mm width.SIST EN 14119:2003
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