EN ISO 12966-4:2015

SLOVENSKI STANDARD
01-september-2015
1DGRPHãþD
SIST EN ISO 15304:2002
SIST EN ISO 15304:2002/AC:2005
SIST EN ISO 5508:1996
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD3OLQVNDNURPDWRJUDILMDPHWLOQLKHVWURY
PDãþREQLKNLVOLQGHO'RORþHYDQMHVNDSLODUQRSOLQVNRNURPDWRJUDIVNRPHWRGR
,62
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters -
Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)
Tierische und pflanzliche Fette und Öle - Gaschromatographie von
Fettsäuremethylestern - Teil 4: Bestimmung von cis-, trans-, gesättigten, mono- und
mehrfach ungesättigten Fettsäuren in pflanzlichen oder nicht-ruminanten Ölen und
Fetten (ISO 12966-4:2015)
Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des
esters méthyliques d'acides gras - Partie 4: Détermination des acides gras saturés,
mono- et poly-insaturés, cis ou trans, dans les corps gras d'origines végétale ou animale
(non ruminant)
Ta slovenski standard je istoveten z: EN ISO 12966-4:2015
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 12966-4
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2015
ICS 67.200.10 Supersedes EN ISO 15304:2002, EN ISO 5508:1995
English Version
Animal and vegetable fats and oils - Gas chromatography of fatty
acid methyl esters - Part 4: Determination by capillary gas
chromatography (ISO 12966-4:2015)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle -
Chromatographie en phase gazeuse des esters Gaschromatographie von Fettsäuremethylestern - Teil 4:
méthyliques d'acides gras - Partie 4: Détermination par Bestimmung mittels Kapillargaschromatographie (ISO
chromatographie capillaire en phase gazeuse (ISO 12966- 12966-4:2015)
4:2015)
This European Standard was approved by CEN on 16 April 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12966-4:2015 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 12966-4:2015) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and
oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by December 2015, and conflicting national standards shall be withdrawn
at the latest by December 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 5508:1995, EN ISO 15304:2002.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 12966-4:2015 has been approved by CEN as EN ISO 12966-4:2015 without any modification.
INTERNATIONAL ISO
STANDARD 12966-4
First edition
2015-06-01
Animal and vegetable fats and oils —
Gas chromatography of fatty acid
methyl esters —
Part 4:
Determination by capillary gas
chromatography
Corps gras d’origines animale et végétale — Chromatographie en
phase gazeuse des esters méthyliques d’acides gras —
Partie 4: Détermination par chromatographie capillaire en phase
gazeuse
Reference number
ISO 12966-4:2015(E)
©
ISO 2015
ISO 12966-4:2015(E)
© ISO 2015, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2015 – All rights reserved

ISO 12966-4:2015(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Reagents and materials . 1
4.1 Reference fatty acid methyl esters (FAMEs) . 2
4.2 Internal standards . 2
5 Apparatus . 3
6 Sampling . 4
7 Preparation of test sample . 4
8 Preparation of methyl esters from fats, oils, and fatty acids . 4
9 Procedure. 4
9.1 General . 4
9.2 GC conditions . 4
9.3 Performance check . 5
10 Calculations. 5
10.1 Qualitative analysis and peak identification . 5
10.2 Quantitative analysis . 5
10.2.1 Calculation of the composition of fatty acid methyl esters . 5
10.2.2 Calculation of the composition of fatty acid methyl esters using
correction factors . 6
10.2.3 Calculation of the composition of fatty acid methyl esters using an
internal standard . 6
11 Precision . 7
11.1 Results of interlaboratory test . 7
11.2 Repeatability . 7
11.3 Reproducibility . 7
12 Test report . 7
Annex A (informative) Theoretical flame ionization detector correction factor (TCF) for
fatty acid methyl esters (FAMEs) . 9
Annex B (informative) Examples of chromatograms .10
Annex C (informative) Comparison of FAME composition with two different GC columns.12
Annex D (informative) Results of an interlaboratory trial .14
Bibliography .20
ISO 12966-4:2015(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document
...