ISO/TC 126 - Tobacco and tobacco products

This document specifies an atmosphere for conditioning and testing samples of fine-cut tobacco and test pieces made from it. It is applicable to tests on fine-cut tobacco and products and materials used in the manufacture of fine-cut smoking articles for which a prior conditioning is necessary. It is not applicable in the case of test methods for which particular test conditions are laid down elsewhere, for example other tobacco products which are given in ISO 3402.

This document specifies a method for the gas chromatographic determination of menthol in the total particulate matter (TPM) of mentholated cigarette mainstream smoke with a smoking regime according to ISO 3308 (standard smoking regime). The smoking of cigarettes and the collection of TPM from the mainstream smoke are carried out in accordance with ISO 4387 with some exceptions (see Annex A). Encapsulated menthol in specific products can lead to specific handling not described in this document.

Le présent document spécifie des méthodes pour le dosage de la matière particulaire totale présente dans le courant principal de fumée d'objets à fumer à base de tabac à rouler (OFTR) au moyen d'une machine à fumer analytique de routine. Il spécifie également la préparation pour le dosage de l'eau et de la nicotine, ainsi que le calcul de la matière particulaire anhydre et exempte de nicotine dans la matière particulaire totale recueillie. Le présent document spécifie la méthode de confection des objets à fumer à base de tabac à rouler à l'aide d’un tube provenant d’une enveloppe spécifiée.

This document specifies a method for the determination of pH of nicotine pouches.

This document: — defines the parameters and specifies the standard conditions for the routine analytical generation and collection of aerosol from electrically heated tobacco products (eHTPs); — specifies technical requirements for the routine analytical puffing machine for eHTP generation and collection of aerosol, termed as “machine” in this document, conforming with the standard conditions stated within Clause 4. This document does not specify aerosol trapping nor subsequent sample preparation and analytical method of components in the trapped aerosol or the gas phase. This document is also applicable to products other than those defined in 3.15 if a specific method references this document.

This document: — defines the parameters and specifies the standard conditions for the routine analytical generation and collection of aerosol from carbon heated tobacco products (cHTP); — specifies technical requirements for the routine analytical puffing machine for cHTP generation and collection of aerosol, termed as “machine” in this document, conforming with the standard conditions stated within Clause 4; This document does not specify aerosol trapping nor subsequent sample preparation and analytical method of components in the trapped aerosol or the gas phase. This document is also applicable to products other than defined in 3.14 if a specific method references this document.

This document: — defines the parameters and specifies the standard conditions for the routine analytical generation and collection of aerosol from aerosol heated tobacco products (aHTP); — specifies technical requirements for the routine analytical puffing machine for aHTP generation and collection of aerosol, termed as “machine” in this document, conforming with the standard conditions stated within Clause 4; This document does not specify aerosol trapping nor subsequent sample preparation and analytical method of components in the trapped aerosol or the gas phase. This document is also applicable to products other than those defined in 3.15 if a specific method references this document.

This document: — defines standardized terminology and definitions for the heated tobacco product (HTP) category; — defines the heated tobacco product (HTP) sub-categories and the attributes of each; — does not include waterpipe or loose-leaf tobacco products. This document does not specify the means for aerosol trapping, subsequent sample preparation or analyses of components in the trapped aerosol. The HTP category clearly excludes products such as conventional combustible cigarettes, cigars, roll-your own tobacco products, pipe tobacco products, e‑vapor products (also called electronic cigarettes or e‑cigarettes), moist smokeless tobacco, oral tobacco products, and oral tobacco derived nicotine (OTDN) products. This document describes the terms and definitions that apply to tobacco heating systems. Any product that falls under those terms and definitions can be tested applying testing standards that are specifically addressing tobacco heating systems. This document aids in choosing the right standard for tobacco heating systems testing.

This document specifies a method for the determination of the filling power of the tobacco used for fine-cut tobacco smoking articles.

This document specifies a method for the determination of carbon monoxide (CO) in the vapour phase of mainstream cigarette smoke collected with the smoking regime specified in ISO 4387.

This document specifies an ion chromatographic method for the determination of the acetate and citrate content of materials used to produce wrappings for cigarette filters, cigarettes, and other tobacco products.

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter of cigarette mainstream smoke with an intense smoking regime using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. This method is specified using ISO 20778 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 23906‑1, with a different clean-up using methanol solvent.

This document specifies the atmosphere for the conditioning and testing of samples of tobacco and tobacco products. It is primarily applicable to cigarettes; however, if not specified differently in other ISO standards, it can also be applied to tobacco, other tobacco products, and materials used in the manufacture of tobacco products for which prior conditioning is necessary. Standards for the conditioning and testing of other forms of tobacco, tobacco products, and materials can refer to all or part of this document.

This document specifies methods for the determination of total particulate matter and preparation for the subsequent determination of nicotine-free dry particulate matter present in the smoke from articles made from fine-cut tobacco and expanded tobacco, generated and collected using a routine analytical smoking machine. It specifies the method of making of fine-cut tobacco smoking articles using a tube made from a specified wrapper.

This document specifies a method of measurement of the masses of e-liquid vaporised and the aerosol collected from vapour product(s). It does not specify the vapour product(s), the vapour product(s) operational settings or, e-liquid to be used. NOTE Application of this document can be required as a preliminary step for subsequent analyses.

This document specifies a method for the determination of the amount of selected carbonyl compounds (formaldehyde and acetaldehyde) as their 2,4-dinitrophenylhydrazones in vapour product emissions using reversed phase liquid chromatography coupled with ultraviolet or diode array detector (LC-UV or LC-DAD). This document does not include the analysis of other carbonyl compounds, such as acrolein and crotonaldehyde, due to previous work indicated issues associated with stability of these compounds in the e-liquid solutions that were used to evaluate method performance[4].

This document specifies an analytical method to quantify nicotine of collected vapour product emissions by gas chromatography.

This document specifies a test method for testing the capability of a cigarette, positioned on one of three standard substrates, to extinguish or to generate sufficient heat to continue burning, and thus potentially cause ignition of bedding or upholstered furniture. This document is only applicable to factory-made cigarettes that burn along the length of a tobacco column. This is a performance-based document; it does not prescribe any design features of the cigarette that can lead to improved or degraded performance in the test method. The output of this method has been correlated with the potential for cigarettes to ignite upholstered furniture.

This document recollects and evaluates physical parameters of filter paper substrates for the determination of ignition propensity of cigarettes according to ISO 12863.

This document specifies a gas-chromatographic (GC) method for the determination of water content. It is applicable to raw tobacco as well as tobacco taken from finished products. The method is suitable for water contents ranging at least from a mass fraction of 2 % to 55 %.

This document describes the requirements for a monitor test piece for smoking machine as well as its use.

This document specifies a method for the determination of water content by the Karl Fischer method. It is applicable to raw tobacco as well as tobacco taken from finished products. The method is suitable for water contents ranging from a mass fraction of at least 2 % to 55 %.

This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISO 4387. NOTE ISO 20778 and ISO 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in tobacco and the following tobacco products: cigarettes, cigars and smokeless tobacco products using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The TSNAs determined with this method are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry (GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of cigarette mainstream smoke with the intense smoking regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC-MS) with methanol as extraction solvent. This method is specified using ISO 20778 smoking parameters. NOTE An alternative method for the determination of B[a]P is specified in ISO 23906-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones by reversed phase HPLC-UV/DAD in mainstream smoke using an intense smoking regime.

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 20778 smoking parameters. This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 20778 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 20 mg/cigarette and 45 mg/cigarette.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 3308 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 1 mg/cigarette and 16 mg/cigarette.

This document specifies a method for the determination of the content of total alkaloids as nicotine in tobacco by continuous-flow analysis. This method is applicable to leaf samples, stems, reconstituted tobacco sheet materials and tobacco blends.

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 3308 smoking parameters. This method is applicable to cigarettes with ammonia yields between 1 µg/cigarette and 30 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

This document specifies a method for the gravimetric determination of the purity of nicotine using tungstosilicic acid. The method is applicable to pure nicotine or nicotine salts used to calibrate analytical methods for the determination of nicotine in the field of tobacco, tobacco products and smoke analysis.

This document specifies the general principles to be applied when sampling batches of raw tobacco in order to assess either — the mean value of one or more of its characteristics, or — the heterogeneity of one or more of its characteristics. Manufactured tobacco products, including products intended for sale or distribution, are specifically not included in the scope of this document. This document is applicable to the sampling of batches of raw tobacco of the following types: a) leaf tobacco: flue cured; air cured; sun cured; fire cured; b) pretreated raw tobacco: which has undergone fermentation (in packages not intended for retail or wholesale sales or distribution, in bulk, in chambers); which has been partially or completely stemmed; which is in the form of stems; which is in the form of waste and remnants; which has been reconstituted in the form of strips.

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. This method is specified using ISO 3308 smoking parameters. This document provides an alternative method to that specified in ISO 22634-1, with a different clean-up, and a shorter total analytical run allowing a potential increase of sample throughput in comparison with ISO 22634-1.

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with methanol as extraction solvent. This method is specified using ISO 3308 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 22634-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

This document specifies a method for determination of carbon monoxide (CO) in the vapour phase of cigarette smoke with an intense smoking regime.

This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

This document specifies an analytical method to quantify the nicotine, propylene glycol and glycerol content in e-liquids by gas chromatography.

This document specifies a method for the determination of the width of strands of cut tobacco. This method is only applicable on samples of strands of cut tobacco with a uniform cut width. NOTE There are other ways of measuring the width of the strands of cut tobacco. A system with the same accuracy can be used, for example a microscope with an internal fitted ruler or a camera with image processing system.

This document specifies a method for the determination of air permeability (AP). It is applicable to materials used as cigarette papers, filter plug wrap and filter joining paper, including materials having an oriented permeable zone or discrete permeable zones where the measured permeability is in excess of 10 cm3·(min−1·cm−2) at 1 kPa. In addition, it is applicable to banded cigarette papers, with bands of a width of at least 4 mm. NOTE For an estimate of the air permeability of materials outside the scope of this document, see Note 3 in 5.1.2 and 7.6.1.4.

This document specifies a method for the gas-chromatographic determination of water in total particulate matter from the mainstream smoke. The smoking of cigarettes and collection of mainstream smoke are normally carried out in accordance with ISO 4387. However, the method of this document is also applicable to the determination of water in total particulate matter from the mainstream smoke obtained by non-standard smoking. NOTE In countries not in a position to use the gas-chromatographic method, the determination of water in total particulate matter from the mainstream smoke is performed using the Karl Fischer method (see ISO 10362-2). In such cases, values obtained for water in total particulate matter from the mainstream smoke are used with the addition of a note made in the expression of the results.

This document specifies a method for the determination of ventilation which is applicable to cigarettes.

This document specifies a method for the determination of carbon monoxide (CO) emission of glowing water pipe charcoal.