ISO/TS 22487:2019

TECHNICAL ISO/TS
SPECIFICATION 22487
First edition
2019-05
Water pipe tobacco — Determination
of total collected matter and nicotine
using a water pipe tobacco smoking
machine
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Apparatus . 2
6 Sampling . 2
7 Determination of total collected matter . 3
7.1 Preparation of the water pipe tobacco for smoking . 3
7.1.1 General. 3
7.1.2 Replicate test portions . 3
7.2 Storage and conditioning . 3
7.3 Preliminary measurement before smoking . 3
7.4 Smoking and collection of collected matter . 3
7.4.1 Preparation of smoke traps . 3
7.4.2 Setting up the smoking machine . 3
7.4.3 Procedure for smoking run . 4
7.5 Determination of total collected matter . 5
7.6 Calculation of total collected matter . 5
7.7 Handling of glass fibre filter . 5
7.8 Determination of nicotine . 5
8 Test report . 6
9 Repeatability and reproducibility . 7
Bibliography . 8
Foreword
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This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
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iv © ISO 2019 – All rights reserved

Introduction
Tobacco smoke is a complex mixture consisting of many individual chemical constituents. These
compounds exist as gases, vapours and condensed aerosol particles. Additionally, various rapid ageing
processes, together with diffusional and intersolubility effects, start occurring immediately after the
formation of the smoke which further complicates its composition. These processes and effects are
particularly relevant to water pipe tobacco smoke where the smoke ages and passes through a water
trap before it reaches the smoker.
Historically, when tobacco products are smoked in a laboratory setting, the particulate matter in smoke
is collected on a glass fibre filter and this approach has been followed in this document for water pipe
tobacco smoking.
The parameters used for “puffing” on the laboratory water pipe used in this document are based on
published studies of human behaviour and data reported to the ISO/TC 126. It is convenient to use the
term “puffing”, however it is, in strict physiological terms, incorrect. Smokers of cigarettes and many
other tobacco products use a two-step process to draw the smoke from the product into the mouth (the
puff), followed usually by inhalation of ambient air into the lungs through either the nose or mouth.
Smokers of water pipes use a one-step process to inhale smoke directly into the lungs.
It is important to note that no machine smoking regime can represent all human smoking behaviour:
— machine smoking testing is useful to characterize water pipe tobacco emissions for design and
regulatory purposes, but communication of machine measurements to smokers can result in
misunderstandings about differences in exposure and risk across brands;
— smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using ISO standards.
TECHNICAL SPECIFICATION ISO/TS 22487:2019(E)
Water pipe tobacco — Determination of total collected
matter and nicotine using a water pipe tobacco smoking
machine
1 Scope
This document specifies methods for the determination of total collected matter and for the subsequent
determination of nicotine present in the smoke from water pipe tobacco generated and collected using
a water pipe tobacco smoking machine.
This document is only applicable for devices known as “arghile”, “hookah”, “nargile” or “shisha” in which
tobacco is only heated, not combusted. Other types, such as “Chinese water pipe”, are not covered.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 10315, Cigarettes — Determination of nicotine in smoke condensates — Gas-chromatographic method
ISO 22486, Water pipe tobacco smoking machine — Definitions and standard conditions
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https: //www .iso .org/obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
total collected matter
TCM
portion of the mainstream smoke which is trapped in the smoke trap (3.2), expressed as milligrams
3.2
smoke trap
device for collecting such parts of the smoke from a sample as is necessary for determination of
specified smoke components
[SOURCE: ISO 3308:2012, 3.15]
3.3
smoking process
use of a smoking machine to smoke the water pipe tobacco from first to final puff
3.4
smoking run
specific smoking process (3.3) to produce such smoke from a sample of water pipe tobacco as is
necessary for the determination of the smoke components
3.5
test portion
water pipe tobacco prepared for a single determination
4 Principle
The water pipe tobacco is sampled and then smoked on a water pipe tobacco smoking machine with
collection of total collected matter in a glass fibre filter trap. The mass of the total collected matter
so collected is determined gravimetrically. The total collected matter is extracted from the trap for
determination of nicotine content by gas chromatography.
In laboratories that are not in a position to use gas-chromatographic methods, reference should be ma
...