Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2023)

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This document also provides information on how to use this method for testing substances under temperate conditions.
The chronic test described is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials.
The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 300 Pa at 25 °C.

Bodenbeschaffenheit - Hemmung der Reproduktion von Collembolen (Folsomia candida) durch Verunreinigungen (ISO 11267:2023)

Dieses Dokument legt eines der Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkungen von Bodenverunreinigungen und Substanzen auf die Reproduktion von Folsomia candida Willem bei Aufnahme über die Haut und durch die Nahrung fest. Dieses Dokument enthält ebenfalls Informationen darüber, wie diese Methode für die Prüfung von Stoffen unter gemäßigten Bedingungen anzuwenden ist.
Diese chronische Prüfung ist anwendbar für Böden und Bodenmaterialien unbekannter Beschaffenheit, z. B. von verunreinigten Standorten, beaufschlagten Böden, Böden nach der Sanierung, industriellen, landwirtschaftlichen oder anderen betroffenen Standorten und Abfallmaterialien.
Das Verfahren ist nicht auf flüchtige Substanzen anwendbar, d. h. Substanzen, bei denen H (Henry-Konstante) oder der Luft/Wasser-Verteilungskoeffizient größer als 1 ist oder bei denen der Dampfdruck 300 Pa bei 25 °C überschreitet.

Qualité du sol - Inhibition de la reproduction de Collembola (Folsomia candida) par des contaminants du sol (ISO 11267:2023)

Le présent document spécifie l’une des méthodes permettant d’évaluer la fonction d’habitat des sols et de déterminer les effets de contaminants du sol et de substances sur la reproduction de Folsomia candida Willem par absorption cutanée et ingestion. Le présent document fournit des informations sur la manière d’utiliser cette méthode pour évaluer des substances dans des conditions tempérées.
L’essai chronique décrit s’applique aux sols et matériaux du sol de qualité inconnue, par exemple provenant de sites pollués, de sols amendés, de sols après remédiation, de sites industriels, agricoles ou d’autres sites d’intérêt et de déchets.
La méthode ne s’applique pas aux substances volatiles, c’est-à-dire aux substances pour lesquelles H (constante de Henry) ou le coefficient de partage air/eau est supérieur à 1, ou pour lesquelles la pression de vapeur excède 300 Pa à 25 °C.

Kakovost tal - Zaviranje razmnoževanja vrste Folsomia candida iz rodu skakačev (Collembola) zaradi onesnaževal v tleh (ISO 11267:2023)

Ta dokument določa eno od metod za ocenjevanje funkcije tal habitata ter določevanje učinkov onesnaževal in snovi v tleh na razmnoževanje vrste Folsomia candida iz rodu skakačev (Collembola) z dermalnim in prehranskim vnosom. Prav tako zagotavlja informacije o uporabi te metode za preskušanje snovi v zmernih pogojih.
Opisan kronični preskus se uporablja za tla in talne materiale neznane kakovosti, na primer tla iz onesnaženih območij, obogatena tla, tla po sanaciji, industrijska, kmetijska in druga problematična mesta ter odpadne materiale.
Metoda se ne uporablja za hlapljive snovi, npr. snovi, pri katerih je H (Henryjeva konstanta) ali koeficient deleža zraka/vode večji od 1 oziroma parni tlak pri 25 °C presega 300 Pa.

General Information

Status
Published
Publication Date
15-Aug-2023
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
16-Aug-2023
Completion Date
16-Aug-2023

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SLOVENSKI STANDARD
01-junij-2024
Nadomešča:
SIST EN ISO 11267:2014
Kakovost tal - Zaviranje razmnoževanja vrste Folsomia candida iz rodu skakačev
(Collembola) zaradi onesnaževal v tleh (ISO 11267:2023)
Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil
contaminants (ISO 11267:2023)
Bodenbeschaffenheit - Hemmung der Reproduktion von Collembolen (Folsomia candida)
durch Verunreinigungen (ISO 11267:2023)
Qualité du sol - Inhibition de la reproduction de Collembola (Folsomia candida) par des
contaminants du sol (ISO 11267:2023)
Ta slovenski standard je istoveten z: EN ISO 11267:2023
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11267
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2023
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 11267:2014
English Version
Soil quality - Inhibition of reproduction of Collembola
(Folsomia candida) by soil contaminants (ISO
11267:2023)
Qualité du sol - Inhibition de la reproduction de Bodenbeschaffenheit - Hemmung der Reproduktion
Collembola (Folsomia candida) par des contaminants von Collembolen (Folsomia candida) durch
du sol (ISO 11267:2023) Verunreinigungen (ISO 11267:2023)
This European Standard was approved by CEN on 30 July 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11267:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11267:2023) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2024, and conflicting national standards
shall be withdrawn at the latest by February 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11267:2014.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 11267:2023 has been approved by CEN as EN ISO 11267:2023 without any modification.

INTERNATIONAL ISO
STANDARD 11267
Third edition
2023-08
Soil quality — Inhibition of
reproduction of Collembola (Folsomia
candida) by soil contaminants
Qualité du sol — Inhibition de la reproduction de Collembola
(Folsomia candida) par des contaminants du sol
Reference number
ISO 11267:2023(E)
ISO 11267:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 11267:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents and material . 4
6 Apparatus . 6
7 Procedure .6
7.1 Experimental design . 6
7.1.1 General . 6
7.1.2 Range-finding test . 6
7.1.3 Definitive test . 7
7.2 Preparation of test mixture . 7
7.2.1 Testing contaminated soil . 7
7.2.2 Testing substances added to the test substrate . 8
7.2.3 Preparation of control container . 8
7.3 Addition of the biological material . 9
7.4 Test conditions and measurements . 9
7.5 Determination of surviving Collembola . 9
8 Calculation and expression of results . 9
8.1 Calculation . 9
8.2 Expression of results . . 9
9 Validity of the test .10
10 Statistical analysis .10
10.1 General . 10
10.2 Single-concentration tests . 10
10.3 Multi-concentration tests . 11
10.3.1 Range-finding test . 11
10.3.2 Definitive test . 11
11 Test report .12
Annex A (informative) Techniques for rearing and breeding Folsomia candida.13
Annex B (normative) Determination of water-holding capacity .15
Annex C (informative) Guidance on adjustment of pH of artificial soil .16
Annex D (informative) Extraction and counting of Collembola .17
Annex E (informative) Specific information of alternative Collembolan species other than
Folsomia candida .18
Bibliography .32
iii
ISO 11267:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO document should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use
of (a) patent(s). IS
...

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