EN ISO 16387:2023
(Main)Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction (ISO 16387:2023)
Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction (ISO 16387:2023)
This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal and alimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality, for example, from contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials.
This document provides information on how to use this method for testing substances under temperate conditions.
The method is not applicable to substances, for which the air/soil partition coefficient is greater than 1, or to substances for which the vapour pressure exceeds 300 Pa at 25 °C.
NOTE No provision is made in the test method for monitoring the persistence of the substance under test.
Bodenbeschaffenheit - Wirkungen von Verunreinigungen auf Enchytraeidae (Enchytraeus sp.) - Bestimmung der Wirkungen auf die Reproduktion (ISO 16387:2023)
Dieses Dokument legt eines der Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkung von Bodenverunreinigungen und Substanzen auf die Reproduktion von Enchytraeus sp. bei Aufnahme über die Haut und durch die Nahrung in einer chronischen Prüfung fest. Es ist anwendbar für Böden und Bodenmaterialien unbekannter Beschaffenheit, zum Beispiel von verunreinigten Standorten, Böden nach der Sanierung, landwirtschaftlichen oder anderen betroffenen Standorten und Abfallstoffen.
Dieses Dokument stellt Informationen bereit, wie dieses Verfahren zur Prüfung von Substanzen unter gemäßigten Bedingungen anzuwenden ist.
Das Verfahren ist nicht auf Substanzen anwendbar, bei denen der Luft/Boden-Verteilungskoeffizient größer als 1 ist, oder bei Substanzen, deren Dampfdruck bei 25 °C 300 Pa überschreitet.
ANMERKUNG Es kann nicht sichergestellt werden, dass die Prüfsubstanz während der gesamten Versuchsdauer stabil bleibt.
Qualité du sol - Effets des contaminants sur les Enchytraeidae (Enchytraeus sp.) - Détermination des effets sur la reproduction (ISO 16387:2023)
Le présent document spécifie l’une des méthodes permettant d’évaluer la fonction d’habitat des sols et de déterminer les effets des contaminants du sol et des substances sur la reproduction d’Enchytraeus sp. par absorption cutanée et ingestion au cours d’un essai chronique. Il est applicable aux sols et aux matériaux de type sol de qualité inconnue provenant, par exemple, de sites contaminés, de sols amendés, de sols après remédiation, de sols agricoles ou d’autres sites d’intérêt, et de déchets.
Le présent document fournit des informations sur la manière d’utiliser cette méthode pour évaluer des substances en conditions tempérées.
Cette méthode ne s’applique pas aux substances pour lesquelles le coefficient de partage air/sol est supérieur à 1, ou pour lesquelles la pression de vapeur à 25 °C est supérieure à 300 Pa.
NOTE La méthode d’essai ne contient aucune disposition permettant de surveiller la persistance de la substance soumise à essai.
Kakovost tal - Vpliv onesnaževal na enhitreje (Enchytraeus sp.) - Ugotavljanje vplivov na razmnoževanje (ISO 16387:2023)
Ta dokument določa eno od metod za ocenjevanje funkcije tal habitata ter določevanje učinkov onesnaževal in snovi v tleh na razmnoževanje vrste Enchytraeus sp. (erhitreje) z dermalnim in prehranskim vnosom v okviru kroničnega preskusa. Uporablja se za tla in talne materiale neznane kakovosti, na primer tla iz onesnaženih območij, obogatena tla, tla po sanaciji, kmetijska in druga problematična mesta ter odpadne materiale.
Dokument zagotavlja informacije o uporabi te metode za preskušanje snovi v zmernih pogojih.
Metoda se ne uporablja za snovi, pri katerih je koeficient deleža zraka/tal večji od 1 oziroma parni tlak pri 25 °C presega 300 Pa.
OPOMBA: Preskusna metoda ne vsebuje določb glede spremljanja obstojnosti snovi, ki se preskuša.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-junij-2024
Nadomešča:
SIST EN ISO 16387:2014
Kakovost tal - Vpliv onesnaževal na enhitreje (Enchytraeus sp.) - Ugotavljanje
vplivov na razmnoževanje (ISO 16387:2023)
Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) -
Determination of effects on reproduction (ISO 16387:2023)
Bodenbeschaffenheit - Wirkungen von Verunreinigungen auf Enchytraeidae
(Enchytraeus sp.) - Bestimmung der Wirkungen auf die Reproduktion (ISO 16387:2023)
Qualité du sol - Effets des contaminants sur les Enchytraeidae (Enchytraeus sp.) -
Détermination des effets sur la reproduction (ISO 16387:2023)
Ta slovenski standard je istoveten z: EN ISO 16387:2023
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 16387
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2023
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 16387:2014
English Version
Soil quality - Effects of contaminants on Enchytraeidae
(Enchytraeus sp.) - Determination of effects on
reproduction (ISO 16387:2023)
Qualité du sol - Effets des contaminants sur les Bodenbeschaffenheit - Wirkungen von
Enchytraeidae (Enchytraeus sp.) - Détermination des Verunreinigungen auf Enchytraeidae (Enchytraeus sp.)
effets sur la reproduction (ISO 16387:2023) - Bestimmung der Wirkungen auf die Reproduktion
(ISO 16387:2023)
This European Standard was approved by CEN on 21 March 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16387:2023 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 16387:2023) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2023, and conflicting national standards shall
be withdrawn at the latest by October 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 16387:2014.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 16387:2023 has been approved by CEN as EN ISO 16387:2023 without any modification.
INTERNATIONAL ISO
STANDARD 16387
Third edition
2023-03
Soil quality — Effects of contaminants
on Enchytraeidae (Enchytraeus sp.) —
Determination of effects on
reproduction
Qualité du sol — Effets des contaminants sur les Enchytraeidae
(Enchytraeus sp.) — Détermination des effets sur la reproduction
Reference number
ISO 16387:2023(E)
ISO 16387:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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Published in Switzerland
ii
ISO 16387:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents and material . 3
6 Apparatus . 6
7 Test environment . 6
8 Procedure .7
8.1 Experimental design . 7
8.1.1 General . 7
8.1.2 Range-finding test . 7
8.1.3 Definitive test . 7
8.2 Preparation of test mixtures . 8
8.2.1 Testing contaminated soil . 8
8.2.2 Testing substances added to the test substrate . 8
8.2.3 Preparation of control container . 9
8.3 Addition of food . 9
8.4 Addition of the biological material . 9
8.5 Test conditions and measurements . 9
8.6 Reference substance . 9
9 Calculation and expression of results .10
9.1 Calculation . 10
9.2 Expression of results . . 10
10 Validity of the test .10
11 Statistical analysis .10
11.1 General . 10
11.2 Range-finding test . 10
11.3 Definitive test . 11
12 Test report .11
Annex A (informative) Conditions for culture of Enchytraeus sp .13
Annex B (informative) Test procedure using other Enchytraeus species .15
Annex C (normative) Determination of total water holding capacity .17
Annex D (informative) Detailed description of extraction techniques .18
Annex E (informative) Overview of the statistical assessment of data (NOEC determination) .20
Bibliography .21
iii
ISO 16387:2023(E)
Foreword
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Attention is drawn to the possibility that some of
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