EN ISO 23611-4:2022
(Main)Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2022)
Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2022)
This document specifies a method for sampling and handling free-living nematodes from terrestrial field soils as a prerequisite for using them as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms).
This document applies to all terrestrial biotopes in which nematodes occur. The sampling design of field studies in general is specified in ISO 18400-101.
This document is not applicable to aquatic nematodes because of differences in the sample matrix (e.g. water column). Methods for some other soil organism groups such as earthworms, collembolans enchytraeids or macro-invertebrates are covered in ISO 23611-1, ISO 23611-2, ISO 23611-3 and ISO 23611-5.
This document does not cover the pedological characterization of the site which is highly recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 include suitable procedures for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme, Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2022)
Dieses Dokument legt ein Verfahren zur Probenahme und Handhabung frei lebender Nematoden aus terrestrischen Freilandböden fest, das als eine Voraussetzung für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) dient.
Dieses Dokument wird angewendet für alle terrestrischen Biotope, in denen Nematoden vorkommen. Die allgemeine Planung der Probenahme für Freilanduntersuchungen ist in ISO 18400 101 festgelegt.
Dieses Dokument ist wegen der Unterschiede in der Probenmatrix (z. B. Wassersäule) nicht auf aquatische Nematoden anwendbar. Methoden für einige andere Gruppen von Bodenorganismen wie Regenwürmer, Collembolen, Enchytraeiden oder Makroinvertebraten werden in ISO 23611 1, ISO 23611 2, ISO 23611 3 und ISO 23611 5 behandelt.
Dieses Dokument behandelt nicht die Charakterisierung des Standortes nach bodenkundlichen Gesichtspunkten, die für die Probenahme von Wirbellosen im Boden nachdrücklich empfohlen wird.ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 behandeln zur Bestimmung des pH Wertes, der Korngrößenverteilung, des C/N Verhältnisses, des Gehaltes an organischem Kohlenstoff und des Wasserrückhaltevermögens geeignetere Verfahren.
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4 : Prélèvement, extraction et identification des nématodes du sol (ISO 23611-4:2022)
Le présent document spécifie une méthode pour échantillonner et manipuler les nématodes du sol en tant que condition préalable pour les utiliser comme bio-indicateurs (par exemple pour évaluer la qualité d’un sol en tant qu’habitat pour des organismes).
Le présent document s’applique à tous les biotopes terrestres dans lesquels des nématodes sont présents. Le mode opératoire d’échantillonnage pour les études de terrain est spécifié de façon générale dans l’ISO 18400-101.
Le présent document n’est pas applicable aux nématodes aquatiques en raison des différences dans la matrice d’échantillonnage (par exemple, la colonne d’eau). Des méthodes destinées à certains autres groupes d’organismes tels que les vers de terre, les collemboles, les enchytréides ou les macro-invertébrés, sont décrites dans l’ISO 23611-1, l’ISO 23611-2, l’ISO 23611-3 et l’ISO 23611-5.
Le présent document ne couvre pas la caractérisation pédologique du site qui est particulièrement recommandée lors du prélèvement d’invertébrés du sol. L’ISO 10390, l’ISO 10694, l’ISO 11272, l’ISO 11274, l’ISO 11277, l’ISO 11461 et l’ISO 11465 décrivent des modes opératoires appropriés pour mesurer le pH, la répartition granulométrique, le rapport C/N, la teneur en carbone organique et la capacité de rétention en eau.
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in identifikacija nematod iz tal (ISO 23611-4:2022)
Ta dokument določa metodo za vzorčenje prostoživečih nematodov v tleh ter rokovanje z njimi kot predpogoj za uporabo teh organizmov kot bioloških indikatorjev (npr. za oceno kakovosti tal kot habitata za organizme).
Ta dokument se uporablja za vse kopenske biotope, v katerih se pojavljajo nematodi. Načrtovanje vzorčenja za terenske raziskave na splošno je podano v standardu ISO 18400-101.
Ta dokument se ne uporablja za vodne nematode zaradi razlik v matrici vzorca (npr. vodni stolpec). Metode za nekatere druge skupine talnih organizmov, kot so deževniki, drobne žuželke ali makro nevretenčarji, so podane v standardih ISO 23611-1, ISO 23611-2, ISO 23611-3 in ISO 23611-5.
Ta dokument ne obravnava pedološke karakterizacije lokacije, ki je zelo priporočljiva pri vzorčenju talnih nevretenčarjev. Za merjenje vrednosti pH, porazdelitve velikosti delcev, razmerja C/N, vsebnosti organskega ogljika in zmogljivosti zadrževanja vode so primernejši postopki v standardih ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 in ISO 11465.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2023
Nadomešča:
SIST EN ISO 23611-4:2012
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in
identifikacija nematod iz tal (ISO 23611-4:2022)
Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and
identification of soil-inhabiting nematodes (ISO 23611-4:2022)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme,
Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2022)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4 : Prélèvement, extraction
et identification des nématodes du sol (ISO 23611-4:2022)
Ta slovenski standard je istoveten z: EN ISO 23611-4:2022
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23611-4
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2022
EUROPÄISCHE NORM
ICS 13.080.30; 13.080.05 Supersedes EN ISO 23611-4:2011
English Version
Soil quality - Sampling of soil invertebrates - Part 4:
Sampling, extraction and identification of soil-inhabiting
nematodes (ISO 23611-4:2022)
Qualité du sol - Prélèvement des invertébrés du sol - Bodenbeschaffenheit - Probenahme von Wirbellosen
Partie 4 : Prélèvement, extraction et identification des im Boden - Teil 4: Probenahme, Extraktion und
nématodes du sol (ISO 23611-4:2022) Bestimmung von Boden bewohnenden Nematoden
(ISO 23611 4:2022)
This European Standard was approved by CEN on 16 July 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
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Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-4:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 23611-4:2022) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2023, and conflicting national standards
shall be withdrawn at the latest by February 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23611-4:2011.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23611-4:2022 has been approved by CEN as EN ISO 23611-4:2022 without any
modification.
INTERNATIONAL ISO
STANDARD 23611-4
Second edition
2022-08
Soil quality — Sampling of soil
invertebrates —
Part 4:
Sampling, extraction and
identification of soil-inhabiting
nematodes
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 4: Prélèvement, extraction et identification des nématodes du
sol
Reference number
ISO 23611-4:2022(E)
ISO 23611-4:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
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Phone: +41 22 749 01 11
Email: copyright@iso.org
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Published in Switzerland
ii
ISO 23611-4:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 3
6.1 Sampling . 3
6.2 Extraction . 4
6.3 Counting . 4
6.4 Fixation and preparation of mass slides . 5
6.5 Identification . 5
7 Procedure .5
7.1 General . 5
7.2 Sampling . 5
7.3 Extraction . 6
7.4 Counting . 7
7.5 Fixation and preparation of mass slides . 8
7.6 Identification . 8
8 Data assessment .8
9 Test report . 9
Annex A (informative) Figures of equipment and methods for nematological research .11
Annex B (informative) Information about the availability of the Oostenbrink elutriator .14
Annex C (informative) Information about the Baermann funnel/tray extraction method .17
Annex D (informative) Examples of the use of soil invertebrates in soil monitoring
programmes (including presentation of their results) .19
Bibliography .24
iii
ISO 23611-4:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/
iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological characterization, in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 444, Environmental characterization of solid matrices, in accordance with
the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 23611-4:2007), which has been
technically revised. The main changes are as follows:
— examples of the use of nematodes in soil monitoring programmes have been added (including
presentation of their results) as an informative annex (see Annex D).
A list of all parts in the ISO 23611 series can be found on the ISO webs
...
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