EN ISO 23611-4:2011

SLOVENSKI STANDARD
01-januar-2012
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Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and
identification of soil-inhabiting nematodes (ISO 23611-4:2007)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme,
Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2007)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4: Prélèvement, extraction et
identification des nématodes du sol (ISO 23611-4:2007)
Ta slovenski standard je istoveten z: EN ISO 23611-4:2011
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 23611-4
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2011
ICS 13.080.30; 13.080.05
English Version
Soil quality - Sampling of soil invertebrates - Part 4: Sampling,
extraction and identification of soil-inhabiting nematodes (ISO
23611-4:2007)
Qualité du sol - Prélèvement des invertébrés du sol - Partie Bodenbeschaffenheit - Probenahme von Wirbellosen im
4: Prélèvement, extraction et identification des nématodes Boden - Teil 4: Probenahme, Extraktion und Bestimmung
du sol (ISO 23611-4:2007) von Boden bewohnenden Nematoden (ISO 23611-4:2007)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-4:2011: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 23611-4:2007 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23611-4:2011 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 23611-4:2007 has been approved by CEN as a EN ISO 23611-4:2011 without any
modification.
INTERNATIONAL ISO
STANDARD 23611-4
First edition
2007-11-15
Soil quality — Sampling of soil
invertebrates —
Part 4:
Sampling, extraction and identification of
soil-inhabiting nematodes
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 4: Prélèvement, extraction et identification des nématodes du sol

Reference number
ISO 23611-4:2007(E)
©
ISO 2007
ISO 23611-4:2007(E)
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ii © ISO 2007 – All rights reserved

ISO 23611-4:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Terms and definitions. 1
3 Principle. 2
4 Reagents. 3
5 Apparatus . 3
5.1 Sampling. 4
5.2 Extraction . 4
5.3 Counting . 4
5.4 Fixation and preparation of mass slides. 5
5.5 Identification. 5
6 Procedure . 5
6.1 General. 5
6.2 Sampling. 5
6.3 Extraction . 6
6.4 Counting . 7
6.5 Fixation and preparation of mass slides. 7
6.6 Identification. 8
7 Data assessment. 8
8 Study report. 9
Annex A (informative) Figures of equipment and methods for nematological research. 10
Annex B (informative) Information about the availability of the Oostenbrink elutriator. 13
Annex C (informative) Information about the Baermann funnel/tray extraction method . 15
Bibliography . 17

ISO 23611-4:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 23611-4 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates:
⎯ Part 1: Hand-sorting and formalin extraction of earthworms
⎯ Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
⎯ Part 3: Sampling and soil extraction of enchytraeids
⎯ Part 4: Sampling, extraction and identification of soil-inhabiting nematodes
iv © ISO 2007 – All rights reserved

ISO 23611-4:2007(E)
Introduction
This part of ISO 23611 has been drawn up since there is a growing need for the standardization of terrestrial
zoological field methods. Such methods, mainly covering the sampling, extraction and handling of soil
invertebrates, are necessary for the following purposes:
[15],[17],[28]
⎯ biological classification of soils including soil quality assessment ;
[9],[10],[13],[24]
⎯ terrestrial bio-indication and long-term monitoring ;
⎯ evaluation of the effects of chemicals on soil animals (ISO 11268-3).
Data for these purposes are gained by standardized methods since they can form the basis for far-reaching
decisions (e.g. whether a given site should be remediated or not). In fact, the lack of such standardized
methods is one of the most important reasons why bio-classification and bio-assessment in terrestrial (i.e. soil)
habitats has so far been relatively rarely used in comparison to aquatic sites.
Nematodes are an important and major part of the soil fauna. Some authors estimate that this group is
probably the most dominant one of the multicellular organisms (Metazoa) on earth. Nematodes occur from the
Antarctic to the tropics and from deep sea sediments to mountain regions. They are active in every place with
sufficient water and organic material. The species diversity and functional variety are impressive. Nematodes
are commonly known as parasites of animals and plants, but the major part of the nematode fauna
participates in decomposition processes by feeding on bacteria and fungi.
Nematodes occur in high numbers [(5 000 to 100 000)/kg fresh soil] and with a high (20 to 100) species
diversity in almost every soil sample. Moreover, there is a broad ecological spectrum of feeding types and
food web relations among the nematodes such as bacterivores, fungivores, herbivores, predators and
[27],[28]
omnivores . These factors make the group highly suitable as indicators for ecological soil
...