EN ISO 23611-5:2024

SLOVENSKI STANDARD
oSIST prEN ISO 23611-5:2023
01-julij-2023
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 5. del: Vzorčenje in ekstrakcija
velikih nevretenčarjev v tleh (ISO/DIS 23611-5:2023)
Soil quality - Sampling of soil invertebrates - Part 5: Sampling and extraction of soil
macro-invertebrates (ISO/DIS 23611-5:2023)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 5: Probenahme
und Extraktion von Makroinvertebraten (Großwirbellosen) im Boden (ISO/DIS 23611-
5:2023)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 5: Prélèvement et extraction
des macro-invertébrés du sol (ISO/DIS 23611-5:2023)
Ta slovenski standard je istoveten z: prEN ISO 23611-5
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
oSIST prEN ISO 23611-5:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN ISO 23611-5:2023
oSIST prEN ISO 23611-5:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 23611-5
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2023-05-24 2023-08-16
Soil quality — Sampling of soil invertebrates —
Part 5:
Sampling and extraction of soil macro-invertebrates
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 5: Prélèvement et extraction des macro-invertébrés du sol
ICS: 13.080.05; 13.080.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
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ISO/DIS 23611-5:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

oSIST prEN ISO 23611-5:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 23611-5
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:

Soil quality — Sampling of soil invertebrates —
Part 5:
Sampling and extraction of soil macro-invertebrates
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 5: Prélèvement et extraction des macro-invertébrés du sol
ICS: 13.080.05; 13.080.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
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NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 23611-5:2023(E)
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ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

oSIST prEN ISO 23611-5:2023
ISO/DIS 23611-5:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principe. 2
5 Reagents . 2
6 Apparatus . 2
7 Field procedure .3
7.1 General . 3
7.2 Collecting macro-invertebrates from the litter zone . 3
7.3 Collecting macro-invertebrates from soil . 4
7.3.1 General . 4
7.3.2 Temperate regions . 4
7.3.3 Tropical regions. 4
8 Laboratory procedure .5
8.1 Treatment of collected samples . 5
8.2 Preservation of specimens . 5
8.3 Biomass determination . 6
9 Assessment of results . 6
10 Test report . 6
Annex A (normative) Background information . 8
Annex B  Sampling soil macro-fauna using Pitfall Traps . 9
[68]
Annex C  Monitoring example with Pitfall Traps (taken from Silva et al. 2015 ) .11
Bibliography .16
iii
oSIST prEN ISO 23611-5:2023
ISO/DIS 23611-5:2023(E)
Foreword
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ISO 23611-5 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil
invertebrates:
— Part 1: Hand-sorting and formalin extraction of earthworms
— Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
— Part 3: Sampling and soil extraction of enchytraeids
— Part 4: Sampling, extraction and identification of soil-inhabiting nematodes
— Part 5: Sampling and extraction of soil macro-invertebrates
— Part 6: Guidance for the design of sampling programmes with soil invertebrates
iv
oSIST prEN ISO 23611-5:2023
ISO/DIS 23611-5:2023(E)
Introduction
This part of ISO 23611 was prepared in response to a need to standardize sampling and extraction
methods for soil macro-invertebrates in several European (temperate) and tropical countries. These
methods are needed for the following purposes:
— biological classification of soils, including soil quality assessment (e.g. References [14], [28] and [37]);
— terrestrial bio-indication and long-term monitoring (e.g. References [65], [74], [75] and [76]).
Data collected using standardized methods can be evaluated more accurately as it allows more reliable
comparison between sites (e.g. polluted vs non-polluted sites, changes in land-use practices).
Soils of the world host an abundance of highly diverse macro-invertebrate communities. Their biology
and ecology have been widely studied. Soil invertebrates are irreplaceable actors of soil formation and
conservation in natural ecosystems. Their relevance to the soil system comes from their abundance
and diversity, and also from their role in key biological processes. They are sensitive indicators of soil
quality and recognized agents of its fertility (e.g. References [58] and [52]). Among the wide diversity of
species, adaptive strategies and size ranges represented, one specific group, also called "soil ecosystem
engineers", includes large invertebrates that actually determine the activities of other smaller
organisms through the mechanical activities they produce in soil (e.g. References [18] and [46]).
Soil macro-invertebrates span a wide range of ecological functions in soil: decomposition of organic
matter, through their own activity and by stimulating the soil's microbiological activity (e.g.
References [2], [3] and [36]), predation that plays an important part in food webs (e.g. References [9],
[51], [56], [59] and [63]), soil aggregation by the production of organo-mineral structures (e.g. nests,
galleries, casts) that can last for days, months or years, and soil bioturbation (e.g. Reference [28]),
etc. These characteristics, coupled with in-depth taxonomic knowledge, has enabled their use as
study organisms in several research programmes dealing with the impacts of forest practices (e.g.
References [11], [36], [47], [57], [60] and [70]) or crop management practices (e.g. References [8], [19],
[27], [29], [30], [33], [38], [55] and [62]). These features make them suitable organisms for use as
bio-indicators of changes in soil quality, especially with respect to land-use practices and pollution (e.g.
References [21], [35], [45], [48], [49], [54], [60] and [74]).
The method proposed in this part of ISO 23611 covers the sampling of all soil macro-invertebrates.
However, the sampling of earthworms is a
...