EN ISO 23611-2:2024

SLOVENSKI STANDARD
01-december-2024
Nadomešča:
SIST EN ISO 23611-2:2012
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 2. del: Vzorčenje in ekstrakcija
mikročlenonožcev: skakači (Collembola) in pršice (Acarina) (ISO 23611-2:2024)
Soil quality - Sampling of soil invertebrates - Part 2: Sampling and extraction of micro-
arthropods (Collembola and Acarina) (ISO 23611-2:2024)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 2: Probenahme
und Extraktion von Mikroarthropoden (Collembolen und Milben) (ISO 23611-2:2024)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 2: Prélèvement et extraction
des micro-arthropodes (Collembola et Acarina) (ISO 23611-2:2024)
Ta slovenski standard je istoveten z: EN ISO 23611-2:2024
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23611-2
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2024
EUROPÄISCHE NORM
ICS 13.080.30; 13.080.05 Supersedes EN ISO 23611-2:2011
English Version
Soil quality - Sampling of soil invertebrates - Part 2:
Sampling and extraction of micro-arthropods (Collembola
and Acarina) (ISO 23611-2:2024)
Qualité du sol - Prélèvement des invertébrés du sol - Bodenbeschaffenheit - Probenahme von Wirbellosen
Partie 2: Prélèvement et extraction des micro- im Boden - Teil 2: Probenahme und Extraktion von
arthropodes (Collembola et Acarina) (ISO 23611- Mikroarthropoden (Collembolen und Milben) (ISO
2:2024) 23611-2:2024)
This European Standard was approved by CEN on 2 May 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-2:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23611-2:2024) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2024, and conflicting national standards
shall be withdrawn at the latest by November 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23611-2:2011.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23611-2:2024 has been approved by CEN as EN ISO 23611-2:2024 without any
modification.
International
Standard
ISO 23611-2
Second edition
Soil quality — Sampling of soil
2024-04
invertebrates —
Part 2:
Sampling and extraction of micro-
arthropods (Collembola and
Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes
(Collembola et Acarina)
Reference number
ISO 23611-2:2024(en) © ISO 2024

ISO 23611-2:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
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CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 23611-2:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Test materials . 2
5.1 Biological material .2
5.2 Reagents .2
6 Apparatus . 3
7 Procedure . 4
7.1 Collecting the soil samples . .4
7.2 Extracting Collembola and Acarina from soil samples .4
7.3 Sorting, preserving and identifying Collembola and Acarina .5
7.3.1 Sorting and preserving .5
7.3.2 Identification .5
8 A ssessment of results . 6
9 Study report . 6
Annex A (informative) Species determination in collembolans and mites . 8
Annex B (informative) Alternative method for sampling of micro-arthropods . 9
Bibliography .10

iii
ISO 23611-2:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
characterization, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 444, Environmental characterization of solid matrices, in accordance with the Agreement
on technical cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 23611-2:2006), which has been technically
revised.
The main changes are as follows:
— an additional Note was added in 7.3.2.1 with the description of an alternative method to the classic pre-
heating techniques for specimen preparation for Collembola taxonomic identification;
— the bibliographic references list was revised and updated.
A list of all parts in the ISO 23611 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
ISO 23611-2:2024(en)
Introduction
This document was prepared in response to a growing need for the standardization of sampling and
extraction methods of soil micro-arthropods. These methods are needed for the following purposes:
— biological classification of soils including soil quality assessment (e.g. References [19], [24], [27], [30],
[36], [40],
...