EN ISO 23611-2:2024

SLOVENSKI STANDARD
oSIST prEN ISO 23611-2:2023
01-julij-2023
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 2. del: Vzorčenje in ekstrakcija
mikročlenonožcev: skakači (Collembola) in pršice (Acarina) (ISO/DIS 23611-2:2023)
Soil quality - Sampling of soil invertebrates - Part 2: Sampling and extraction of micro-
arthropods (Collembola and Acarina) (ISO/DIS 23611-2:2023)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 2: Probenahme
und Extraktion von Mikroarthropoden (Collembolen und Milben) (ISO/DIS 23611-2:2023)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 2: Prélèvement et extraction
des micro-arthropodes (Collembola et Acarina) (ISO/DIS 23611-2:2023)
Ta slovenski standard je istoveten z: prEN ISO 23611-2
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
oSIST prEN ISO 23611-2:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN ISO 23611-2:2023
oSIST prEN ISO 23611-2:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 23611-2
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2023-05-24 2023-08-16
Soil quality — Sampling of soil invertebrates —
Part 2:
Sampling and extraction of micro-arthropods (Collembola
and Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes (Collembola et Acarina)
ICS: 13.080.05; 13.080.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 23611-2:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

oSIST prEN ISO 23611-2:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 23611-2
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:

Soil quality — Sampling of soil invertebrates —
Part 2:
Sampling and extraction of micro-arthropods (Collembola
and Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes (Collembola et Acarina)
ICS: 13.080.05; 13.080.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on STANDARD UNTIL PUBLISHED AS SUCH.
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
IN ADDITION TO THEIR EVALUATION AS
or ISO’s member body in the country of the requester. BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
ISO copyright office
USER PURPOSES, DRAFT INTERNATIONAL
CP 401 • Ch. de Blandonnet 8
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
CH-1214 Vernier, Geneva
POTENTIAL TO BECOME STANDARDS TO
Phone: +41 22 749 01 11
WHICH REFERENCE MAY BE MADE IN
Reference number
Email: copyright@iso.org
NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 23611-2:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

oSIST prEN ISO 23611-2:2023
ISO/DIS 23611-2:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test materials . 2
5.1 Biological material . 2
5.2 Reagents . 2
6 Apparatus . 3
7 Procedure .4
7.1 Collecting the soil samples . . 4
7.2 Extracting Collembola and Acarina from soil samples . 5
7.3 Sorting, preserving and identifying Collembola and Acarina . 5
7.3.1 Sorting and preserving . 5
7.3.2 Identification . 6
8 Assessment of results . 6
9 Study report . 7
Annex A (informative) Species determination in collembolans and mites .8
Annex B (informative) Alternative method for sampling of micro-arthropods .10
Bibliography .11
iii
oSIST prEN ISO 23611-2:2023
ISO/DIS 23611-2:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www.iso.org/iso/foreword.html.
ISO 23611-2 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil
invertebrates:
— Part 1: Hand-sorting and formalin extraction of earthworms
— Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
— Part 3: Sampling and soil extraction of enchytraeids
— Part 4: Sampling, extraction and identification of free-living stages of terrestrial nematodes
iv
oSIST prEN ISO 23611-2:2023
ISO/DIS 23611-2:2023(E)
Introduction
This part of ISO 23611 has been drawn up since there is a growing need for the standardization of
sampling and extraction methods of soil micro-arthropods. These methods are needed for the following
purposes:
— biological classification of soils including soil quality assessment (e.g. References [19], [24], [27],
[30], [36], [40], [41]);
— terrestrial bioindication and long-term monitoring (e.g. References [3], [12], [14], [19], [31], [34],
[37]).
Data collected by standardized methods can be more accurately evaluated allowing more reliable
comparisons between sites (e.g. polluted versus non-polluted sites, changes in land-use practices).
From the several micro-arthropod groups, Collembola and Acarina are the most studied in soil ecology.
Their relevance for the soil system comes from their high abundance and diversity, and also from their
role in key biological processes. Collembola and Oribatid mites act mainly as catalysts in organic matter
[6],[21] [21],[26]
decomposition , whereas predacious mites may act as webmasters in soil food webs . These
characteristics, allied to a widespread taxonomic knowledge, allowed their use as study organisms in
several research programmes dealing with the impacts of forest practices (e.g. References [8], [16], [17],
[18], [22], [23], [24], [28], [29], [32], [33], [35], [42]) or crop management practices (e.g. [2], [7], [10],
[13], [20], [25], [43], [44]). These features make them suitable organisms to be used as bio-indicators of
[38]
changes in soil quality, especially due to land-use practices and pollution .
v
oSIST prEN ISO 23611-2:2023
oSIST prEN ISO 23611-2:2023
DRAFT INTERNATIONAL STANDARD ISO/DIS 23611-2:2023(E)
Soil quality — Sampling of soil invertebrates —
Part 2:
Sampling and extraction of micro-arthropods (Collembola
and Acarina)
1 Scope
This part of ISO 23611 specifies a method for sampling, extracting and preserving collembolans and
mites from field soils as a prerequisite for using these animals as bio-indicators (e.g. to assess the
quality of a soil as a habitat for organisms).
Basic information on the ecology of micro-arthropods and their use can be found in the references
listed in the Bibliography.
The sampling and extraction methods of this part of ISO 23611 are applicable to almost all types of
soils. Exceptions may be soils from extreme climatic conditions (hard, frozen or flooded soils) and other
matrices than soil, e.g. tree trunks, plants or lichens. For the sampling design of field studies in general,
see ISO 18400-104.
Methods for some other soil organism groups, such as earthworms, are covered in other parts of
ISO 23611.
This part of ISO 23611 does not cover the pedological characterization of the site, which is highly
recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274,
ISO 11277, ISO 11461, and ISO 11465 are more suitable for measuring pH, particle size distribution, C/N
ratio, organic carbon content and water-holding capacity.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: availab
...