EN ISO 23611-2:2011

SLOVENSKI STANDARD
01-januar-2012
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Soil quality - Sampling of soil invertebrates - Part 2: Sampling and extraction of micro-
arthropods (Collembola and Acarina) (ISO 23611-2:2006)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 2: Probenahme
und Extraktion von Mikroarthropoden (Collembolen und Milben) (ISO 23611-2:2006)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 2 : Prélèvement et extraction
des micro-arthropodes (Collembola et Acarina) (ISO 23611-2:2006)
Ta slovenski standard je istoveten z: EN ISO 23611-2:2011
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 23611-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2011
ICS 13.080.30; 13.080.05
English Version
Soil quality - Sampling of soil invertebrates - Part 2: Sampling
and extraction of micro-arthropods (Collembola and Acarina)
(ISO 23611-2:2006)
Qualité du sol - Prélèvement des invertébrés du sol - Partie Bodenbeschaffenheit - Probenahme von Wirbellosen im
2 : Prélèvement et extraction des micro-arthropodes Boden - Teil 2: Probenahme und Extraktion von
(Collembola et Acarina) (ISO 23611-2:2006) Mikroarthropoden (Collembolen und Milben) (ISO 23611-
2:2006)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-2:2011: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 23611-2:2006 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23611-2:2011 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 23611-2:2006 has been approved by CEN as a EN ISO 23611-2:2011 without any
modification.
INTERNATIONAL ISO
STANDARD 23611-2
First edition
2006-02-01
Soil quality — Sampling of soil
invertebrates —
Part 2:
Sampling and extraction of
micro-arthropods (Collembola
and Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes (Collembola
et Acarina)
Reference number
ISO 23611-2:2006(E)
©
ISO 2006
ISO 23611-2:2006(E)
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ii © ISO 2006 – All rights reserved

ISO 23611-2:2006(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Terms and definitions. 1
3 Principle. 1
4 Test materials. 2
4.1 Biological material. 2
4.2 Reagents. 2
5 Apparatus. 3
6 Procedure. 4
6.1 Collecting the soil samples . 4
6.2 Extracting Collembola and Acarina from soil samples . 4
6.3 Sorting, preserving and identifying Collembola and Acarina. 5
7 Assessment of results. 6
8 Study report. 6
Annex A (informative) Species determination in collembolans and mites . 7
Annex B (informative) Alternative methods for sampling of micro-arthropods . 9
Bibliography . 10

ISO 23611-2:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 23611-2 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates:
⎯ Part 1: Hand-sorting and formalin extraction of earthworms
⎯ Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
⎯ Part 3: Sampling and soil extraction of enchytraeids
⎯ Part 4: Sampling, extraction and identification of free-living stages of terrestrial nematodes
iv © ISO 2006 – All rights reserved

ISO 23611-2:2006(E)
Introduction
This part of ISO 23611 has been drawn up since there is a growing need for the standardization of sampling
and extraction methods of soil micro-arthropods. These methods are needed for the following purposes:
⎯ biological classification of soils including soil quality assessment (e.g. References [31], [32], [35], [41],
[45], [46]);
⎯ terrestrial bioindication and long-term monitoring (e.g. References [1], [7], [17], [40], [42]).
Data collected by standardized methods can be more accurately evaluated allowing more reliable
comparisons between sites (e.g. polluted versus non-polluted sites, changes in land-use practices).
From the several micro-arthropod groups, Collembola and Acarina are the most studied in soil ecology. Their
relevance for the soil system comes from their high abundance and diversity, and also from their role in key
biological processes. Collembola and Oribatid mites act mainly as catalysts in organic matter decomposition
[4], [20] [9]
, whereas predacious mites may act as webmasters in soil food webs . These characteristics, allied to
a widespread taxonomic knowledge, allowed their use as study organisms in several research programmes
dealing with the impacts of forest practices (e.g. References [12], [13], [14], [15], [18], [19], [21], [22], [23], [25],
[26], [27], [28], [29], [30], [31], [33], [34], [37], [38], [39]) or crop management practices (e.g. [6], [11], [16], [24]).
These features make them suitable organisms to be used as bio-indicators of changes in soil quality,
[43]
especially due to land-use practices and pollution .

INTERNATIONAL STANDARD ISO 23611-2:2006(E)

Soil quality — Sampling of soil invertebrates —
Part 2:
Sampling and extraction of micro-arthropods (Collembola and
Acarina)
1 Scope
This part of ISO 23611 specifies a method for sampling, extracting and preserving collembolans and mites
from field soils as a prerequisite for using these animals as bio-indicators (e.g. to assess the quality of a soil
as a habitat for organisms).
Basic information on the ecology of micro-arthropods and their use can be found in the references listed in the
Bibliography.
The sampling and extraction methods of this part of ISO 23611 are applicable to almost all types of soils.
Exceptions may be soils from extreme climatic conditions (hard, frozen or flooded soils) and other matrices
than soil, e.g. tree trunks, plants or lichens. For the sampling design of field studies in general, see
ISO 10381-1.
Methods for some other soil organism groups such as earthworms are covered in other parts of ISO 23611.
This part of ISO 23611 does not cover the pedological characterization of the site which is highly
recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, IS
...