EN ISO 16387:2014
(Main)Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction (ISO 16387:2014)
Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction (ISO 16387:2014)
ISO 16387:2014 specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal and alimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials.
ISO 16387:2014 provides information on how to use this method for testing substances under temperate conditions.
Bodenbeschaffenheit - Wirkungen von Verunreinigungen auf Enchytraeidae (Enchytraeus sp.) - Bestimmung der Wirkungen auf die Reproduktion (ISO 16387:2014)
Diese Internationale Norm legt eines der Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkung von Bodenverunreinigungen und Substanzen auf die Reproduktion von Enchytraeus sp. bei Aufnahme über die Haut und durch die Nahrung in einer chronischen Prüfung fest. Sie ist anwendbar für Böden und Bodenmaterialien unbekannter Beschaffenheit, z. B. von verunreinigten Standorten, Böden nach der Sanierung, landwirtschaftlichen oder anderen betroffenen Standorten und Abfallmaterialien.
Die Wirkungen der Substanzen werden mit einem Standardboden, vorzugsweise mit einem festgelegten künstlichen Bodensubstrat, bewertet. Bei verunreinigten Böden werden die Wirkungen im zu prüfenden Boden und in einem Kontrollboden bestimmt. Entsprechend der Zielsetzung der Untersuchung sind die Kontrolle und das zur Verdünnung verwendete Substrat (Verdünnungsreihen des verunreinigten Bodens) entweder ein mit dem zu prüfenden Boden vergleichbarer, nicht verunreinigter Boden (Referenzboden) oder ein Standardboden (z. B. künstlicher Boden).
Diese Internationale Norm gibt Informationen, wie dieses Verfahren zur Prüfung von Substanzen unter gemäßigten Bedingungen anzuwenden ist.
Das Verfahren ist nicht auf flüchtige Substanzen anwendbar, d. h. Substanzen, bei denen H (Henry-Konstante) oder der Luft/Wasser-Verteilungskoeffizient größer als 1 ist, oder bei denen der Dampfdruck bei 25 °C 0,013 3 Pa überschreitet.
ANMERKUNG Es kann nicht sichergestellt werden, dass die Prüfsubstanz während der gesamten Versuchsdauer stabil bleibt.
Qualité du sol - Effets des contaminants sur les Enchytraeidae (Enchytraeus sp.) - Détermination des effets sur la survie et la reproduction (ISO 16387:2014)
L'ISO 16387:2014 spécifie l'une des méthodes permettant d'évaluer la fonction d'habitat des sols et de déterminer les effets des contaminants et des substances du sol sur la reproduction d'Enchytraeus sp. par absorption cutanée et ingestion au cours d'un essai chronique. Elle est applicable aux sols et aux matériaux de type sol de qualité inconnue provenant, par exemple, de sites contaminés, de sols amendés, de sols après remédiation, de sols agricoles ou d'autres sites d'intérêt, et de déchets.
L'ISO 16387:2014 fournit des informations sur la manière d'utiliser cette méthode pour évaluer des substances en conditions tempérées.
Kakovost tal - Vpliv onesnaževal na enhitreje (Enchytraeus sp.) - Ugotavljanje vplivov na razmnoževanje (ISO 16387:2014)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2014
Kakovost tal - Vpliv onesnaževal na enhitreje (Enchytraeus sp.) - Ugotavljanje
vplivov na razmnoževanje (ISO 16387:2014)
Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination
of effects on reproduction (ISO 16387:2014)
Bodenbeschaffenheit - Wirkungen von Verunreinigungen auf Enchytraeidae
(Enchytraeus sp.) - Bestimmung der Wirkungen auf die Reproduktion (ISO 16387:2014)
Qualité du sol - Effets des contaminants sur les Enchytraeidae (Enchytraeus sp.) -
Détermination des effets sur la survie et la reproduction (ISO 16387:2014)
Ta slovenski standard je istoveten z: EN ISO 16387:2014
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 16387
NORME EUROPÉENNE
EUROPÄISCHE NORM
January 2014
ICS 13.080.30
English Version
Soil quality - Effects of contaminants on Enchytraeidae
(Enchytraeus sp.) - Determination of effects on reproduction
(ISO 16387:2014)
Qualité du sol - Effets des contaminants sur les Bodenbeschaffenheit - Wirkungen von Verunreinigungen
Enchytraeidae (Enchytraeus sp.) - Détermination des effets auf Enchytraeidae (Enchytraeus sp.) - Bestimmung der
sur la survie et la reproduction (ISO 16387:2014) Wirkungen auf die Reproduktion (ISO 16387:2014)
This European Standard was approved by CEN on 12 October 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
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Kingdom.
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CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16387:2014 E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 16387:2014) has been prepared by Technical Committee ISO/TC 190 “Soil quality” in
collaboration with Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held
by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by July 2014, and conflicting national standards shall be withdrawn at the
latest by July 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 16387:2014 has been approved by CEN as EN ISO 16387:2014 without any modification.
INTERNATIONAL ISO
STANDARD 16387
Second edition
2014-01-15
Soil quality — Effects of contaminants
on Enchytraeidae (Enchytraeus
sp.) — Determination of effects on
reproduction
Qualité du sol — Effets des contaminants sur les Enchytraeidae
(Enchytraeus sp.) — Détermination des effets sur la survie et la
reproduction
Reference number
ISO 16387:2014(E)
©
ISO 2014
ISO 16387:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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ii © ISO 2014 – All rights reserved
ISO 16387:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents and material . 3
6 Apparatus . 6
7 Test environment . 6
8 Procedure. 7
8.1 Experimental design . 7
8.2 Preparation of test mixtures . 8
8.3 Addition of the biological material . 9
8.4 Test conditions and measurements . 9
8.5 Reference substance . 9
9 Calculation and expression of results .10
9.1 Calculation .10
9.2 Expression of results .10
10 Validity of the test .10
11 Statistical analysis .10
11.1 General .10
11.2 Range-finding test .10
11.3 Definitive test .11
12 Test report .11
Annex A (informative) Conditions for culture of Enchytraeus sp .13
Annex B (informative) Test procedure using other Enchytraeus species .15
Annex C (informative) Determination of maximum water-holding capacity .17
Annex D (informative) Detailed description of extraction techniques .18
Annex E (informative) Overview of the statistical assessment of data (NOEC determination) .19
Bibliography .20
ISO 16387:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information.
The committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
This second edition cancels and replaces the first edition (ISO 16387:2004), which has been technically
revised.
iv © ISO 2014 – All rights reserved
ISO 16387:2014(E)
Introduction
Ecotoxicological test systems are applied to obtain information about the effects of contaminants in
soil and are proposed to complement conventional chemical analysis. ISO 15799 includes a list and
short characterization of recommended and standardized test systems. Aquatic test systems with soil
eluate are applied to obtain information about the fraction of contaminants potentially reaching the
groundwater by the water path (
...
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