EN ISO 23266:2021
(Main)Soil quality - Test for measuring the inhibition of reproduction in oribatid mites (Oppia nitens) exposed to contaminants in soil (ISO 23266:2020)
Soil quality - Test for measuring the inhibition of reproduction in oribatid mites (Oppia nitens) exposed to contaminants in soil (ISO 23266:2020)
This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intended to replace the earthworm or Collembola tests since it represents another taxonomic group (= mites; i.e., arachnids), nor the predatory mite test since this species represents a different trophic level and ecological niche.
Effects of substances are assessed using standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil with similar properties to the soil sample to be tested (reference soil) or a standard soil (e.g., artificial soil).
Information is provided on how to use this method for testing substances under temperate conditions.
This document is not applicable to substances for which the air/soil partition coefficient is greater than 1, or to substances with vapour pressure exceeding 300 Pa at 25 °C.
NOTE The stability of the test substance cannot be assured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.
Bodenbeschaffenheit - Verfahren zur Bestimmung der Wirkungen von kontaminierten Böden auf die Reproduktion von Hornmilben (Oppia nitens) (ISO 23266:2020)
Dieses Dokument legt eines der Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkungen von Bodenkontaminationen und einzelnen chemischen Substanzen auf die Reproduktionsleistung der Hornmilbe Oppia nitens bei Aufnahme über die Haut und mit der Nahrung. Diese (28 tägige) Dauerprüfung ist auf Böden und Bodenmaterialien unbekannter Beschaffenheit (z. B. Alt-lasten, veränderte Böden, Böden nach einer Sanierung, landwirtschaftlich genutzte oder andere Böden, bei denen ein Prüfanlass besteht, und Abfallmaterialien) anwendbar. Dieses Verfahren ist weder als Ersatz für Prüfungen mit Regenwürmern oder Collembolen anzuwenden, da es eine andere taxonomische Gruppe (= Milben; d. h. Arachniden) in den Fokus stellt, noch als Ersatz für die Raubmilbenprüfung, da diese Art eine andere trophische Ebene und ökologische Nische repräsentiert.
Die Wirkungen von Substanzen werden unter Verwendung von Standardboden, vorzugsweise einem definier¬ten künstlichen Bodensubstrat, beurteilt. Bei kontaminierten Böden werden die Wirkungen im Prüfboden und in einem Kontrollboden bestimmt. In Abhängigkeit von der Zielsetzung der Untersuchung sollte für den Kontrollansatz und das Substrat zum Strecken bzw. Verdünnen (Verdünnungsreihen von kontaminiertem Boden) entweder ein nicht kontaminierter Boden mit ähnlichen Eigenschaften wie die zu prüfende Boden¬probe (Referenzboden) oder ein Standardboden (z. B. künstlicher Boden) verwendet werden.
Es werden Informationen darüber gegeben, wie dieses Verfahren zur Prüfung von Substanzen unter gemäßig¬ten Bedingungen anzuwenden ist.
Dieses Dokument ist nicht anzuwenden für Substanzen, bei denen der Luft/Boden-Verteilungskoeffizient größer als 1 ist oder bei denen der Dampfdruck bei 25 °C 300 Pa übersteigt.
ANMERKUNG Es kann nicht sichergestellt werden, dass die Prüfsubstanz während der gesamten Prüfdauer stabil bleibt. Bei dem Prüfverfahren sind keine Vorkehrungen zur Überwachung der Persistenz der zu prüfenden Substanz vorgesehen.
WARNUNG — Kontaminierte Böden können unbekannte Gemische von giftigen, mutagenen oder anderweitig schädlichen Chemikalien oder infektiösen Mikroorganismen enthalten. Durch Staub und verdunstete Chemikalien können während der Handhabung und der Inkubation Arbeitsschutzrisiken entstehen. Es sollten Vorkehrungen getroffen werden, um einen Hautkontakt zu vermeiden.
Qualité du sol - Essai de détermination de l'inhibition de la reproduction chez les acariens oribates (Oppia nitens) exposés aux contaminants dans le sol (ISO 23266:2020)
Le présent document spécifie l'une des méthodes d'évaluation de la fonction d'habitat des sols et détermine les effets des contaminants et substances chimiques individuelles du sol sur la reproduction de l'acarien oribate, Oppia nitens, par absorption par voie dermique et alimentaire. Cet essai chronique (28 jours) est applicable aux sols et matériaux de type sol de qualité inconnue (par exemple, sites contaminés, sols amendés, sols après remédiation, sols agricoles ou autres sites sujets à préoccupation et déchets). Cette méthode n'est pas destinée à remplacer ni les essais sur vers de terre ou sur collemboles car elle représente un autre groupe taxonomique (= acariens; à savoir, arachnides), ni l'essai sur acariens prédateurs car cette espèce représente un niveau trophique et une niche écologique différents.
Les effets des substances sont évalués en utilisant un sol standard, de préférence un substrat défini de sol artificiel. Pour les sols contaminés, les effets sont déterminés dans le sol soumis à essai et dans un sol témoin. Selon l'objectif de l'étude, il convient que le substrat témoin et le substrat de dilution (séries de dilutions du sol contaminé) soient un sol non contaminé ayant des propriétés similaires à celles du sol à analyser (sol de référence) ou un sol standard (par exemple, sol artificiel).
Des informations sont fournies sur la façon d'utiliser cette méthode pour analyser les substances dans des conditions tempérées.
Le présent document n'est pas applicable aux substances pour lesquelles le coefficient de partage air/sol est supérieur à 1, ou aux substances dont la pression de vapeur dépasse 300 Pa à 25 °C.
NOTE La stabilité de la substance d'essai ne peut pas être garantie tout au long de la période d'essai. La méthode d'essai ne prévoit pas de contrôler la persistance de la substance soumise à essai.
Kakovost tal - Preskus zaviranja razmnoževanja pršic (Oppia nitens) zaradi izpostavljenosti onesnaževalom v tleh (ISO 23266:2020)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2021
Kakovost tal - Preskus zaviranja razmnoževanja pršic (Oppia nitens) zaradi
izpostavljenosti onesnaževalom v tleh (ISO 23266:2020)
Soil quality - Test for measuring the inhibition of reproduction in oribatid mites (Oppia
nitens) exposed to contaminants in soil (ISO 23266:2020)
Bodenbeschaffenheit - Verfahren zur Bestimmung der Wirkungen von verunreinigten
Böden auf das Überleben und die Reproduktion von Hornmilben (Oppia nitens) (ISO
23266:2020)
Qualité du sol - Essai de détermination de l'inhibition de la reproduction chez les
acariens oribates (Oppia nitens) exposés aux contaminants dans le sol (ISO
23266:2020)
Ta slovenski standard je istoveten z: EN ISO 23266:2021
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23266
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2021
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Test for measuring the inhibition of
reproduction in oribatid mites (Oppia nitens) exposed to
contaminants in soil (ISO 23266:2020)
Qualité du sol - Essai de détermination de l'inhibition Bodenbeschaffenheit - Verfahren zur Bestimmung der
de la reproduction chez les acariens oribates (Oppia Wirkungen von verunreinigten Böden auf das
nitens) exposés aux contaminants dans le sol (ISO Überleben und die Reproduktion von Hornmilben
23266:2020) (Oppia nitens) (ISO 23266:2020)
This European Standard was approved by CEN on 5 April 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23266:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 23266:2020 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23266:2021 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2021, and conflicting national standards shall
be withdrawn at the latest by October 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 23266:2020 has been approved by CEN as EN ISO 23266:2021 without any modification.
INTERNATIONAL ISO
STANDARD 23266
First edition
2020-06
Soil quality — Test for measuring the
inhibition of reproduction in oribatid
mites (Oppia nitens) exposed to
contaminants in soil
Qualité du sol — Essai de détermination de l'inhibition de la
reproduction chez les acariens oribates (Oppia nitens) exposés aux
contaminants dans le sol
Reference number
ISO 23266:2020(E)
©
ISO 2020
ISO 23266:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
ISO 23266:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 4
5 Reagents and material . 4
6 Apparatus . 7
7 Procedure. 7
7.1 Experimental design . 7
7.1.1 General. 7
7.1.2 Range-finding test (preliminary test) . 7
7.1.3 Definitive test . 8
7.2 Preparation of test mixture . 8
7.2.1 Testing contaminated soil . 8
7.2.2 Testing substances added to the test substrate . 9
7.2.3 Preparation of control containers .10
7.3 Addition of the mites .10
7.4 Test conditions and measurements .10
7.5 Determination of adult survival and reproductive output .10
8 Calculation and expression of results .11
8.1 Calculation .11
8.2 Expression of results .11
9 Validity of the test .11
10 Statistical analysis .12
10.1 General .12
10.2 Single-concentration tests .12
10.3 Multi-concentration tests.12
10.3.1 Range-finding test .12
10.3.2 Definitive test .12
11 Test report .13
Annex A (informative) Techniques for rearing and breeding of Oppia nitens .15
Annex B (normative) Determination of water holding capacity .18
Annex C (informative) Guidance on adjustment of pH of artificial soil .19
Annex D (informative) Extraction and counting of Oppia nitens .20
Annex E (informative) Performance of the method .24
Bibliography .29
ISO 23266:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible
...
SLOVENSKI STANDARD
01-julij-2021
Kakovost tal - Preskus zaviranja razmnoževanja pršic (Oppia nitens) zaradi
izpostavljenosti onesnaževalom v tleh (ISO 23266:2020)
Soil quality - Test for measuring the inhibition of reproduction in oribatid mites (Oppia
nitens) exposed to contaminants in soil (ISO 23266:2020)
Bodenbeschaffenheit - Verfahren zur Bestimmung der Wirkungen von kontaminierten
Böden auf die Reproduktion von Hornmilben (Oppia nitens) (ISO 23266:2020)
Qualité du sol - Essai de détermination de l'inhibition de la reproduction chez les
acariens oribates (Oppia nitens) exposés aux contaminants dans le sol (ISO
23266:2020)
Ta slovenski standard je istoveten z: EN ISO 23266:2021
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23266
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2021
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Test for measuring the inhibition of
reproduction in oribatid mites (Oppia nitens) exposed to
contaminants in soil (ISO 23266:2020)
Qualité du sol - Essai de détermination de l'inhibition Bodenbeschaffenheit - Verfahren zur Bestimmung der
de la reproduction chez les acariens oribates (Oppia Wirkungen von verunreinigten Böden auf das
nitens) exposés aux contaminants dans le sol (ISO Überleben und die Reproduktion von Hornmilben
23266:2020) (Oppia nitens) (ISO 23266:2020)
This European Standard was approved by CEN on 5 April 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23266:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 23266:2020 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23266:2021 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2021, and conflicting national standards shall
be withdrawn at the latest by October 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 23266:2020 has been approved by CEN as EN ISO 23266:2021 without any modification.
INTERNATIONAL ISO
STANDARD 23266
First edition
2020-06
Soil quality — Test for measuring the
inhibition of reproduction in oribatid
mites (Oppia nitens) exposed to
contaminants in soil
Qualité du sol — Essai de détermination de l'inhibition de la
reproduction chez les acariens oribates (Oppia nitens) exposés aux
contaminants dans le sol
Reference number
ISO 23266:2020(E)
©
ISO 2020
ISO 23266:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
ISO 23266:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 4
5 Reagents and material . 4
6 Apparatus . 7
7 Procedure. 7
7.1 Experimental design . 7
7.1.1 General. 7
7.1.2 Range-finding test (preliminary test) . 7
7.1.3 Definitive test . 8
7.2 Preparation of test mixture . 8
7.2.1 Testing contaminated soil . 8
7.2.2 Testing substances added to the test substrate . 9
7.2.3 Preparation of control containers .10
7.3 Addition of the mites .10
7.4 Test conditions and measurements .10
7.5 Determination of adult survival and reproductive output .10
8 Calculation and expression of results .11
8.1 Calculation .11
8.2 Expression of results .11
9 Validity of the test .11
10 Statistical analysis .12
10.1 General .12
10.2 Single-concentration tests .12
10.3 Multi-concentration tests.12
10.3.1 Range-finding test .12
10.3.2 Definitive test .12
11 Test report .13
Annex A (informative) Techniques for rearing and breeding of Oppia nitens .15
Annex B (normative) Determination of water holding capacity .18
Annex C (informative) Guidance on adjustment of pH of artificial soil .19
Annex D (informative) Extraction and counting of Oppia nitens .20
Annex E (informative) Performance of the method .24
Bibliography .29
ISO 23266:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying an
...
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