EN ISO 11267:2014
(Main)Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2014)
Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2014)
ISO 11267:2014 specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials.
Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil).
ISO 11267:2014 provides information on how to use this method for testing substances under temperate conditions.
The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.
Bodenbeschaffenheit - Hemmung der Reproduktion von Collembolen (Folsomia candida) durch Verunreinigungen (ISO 11267:2014)
Diese Internationale Norm legt eines der Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkungen von Bodenverunreinigungen und Substanzen auf die Reproduktion von Folsomia candida Willem bei Aufnahme über die Haut und durch die Nahrung fest. Diese chronische Prüfung ist anwendbar für Böden und Bodenmaterialien unbekannter Beschaffenheit, z. B. von verunreinigten Standorten, beaufschlagten Böden, Böden nach der Sanierung, industriellen, landwirtschaftlichen oder anderen betroffenen Standorten und Abfallmaterialien.
Die Wirkungen der Substanzen werden mit einem Standardboden, vorzugsweise mit einem festgelegten künstlichen Bodensubstrat, bewertet. Bei verunreinigten Böden werden die Wirkungen im zu prüfenden Boden und in einem Kontrollboden bestimmt. Entsprechend der Zielsetzung der Untersuchung sind die Kontrolle und das zur Verdünnung verwendete Substrat (Verdünnungsreihen des verunreinigten Bodens) entweder ein mit dem zu prüfenden Boden vergleichbarer, nicht verunreinigter Boden (Referenzboden) oder ein Standardboden (z. B. künstlicher Boden).
Diese Internationale Norm gibt Informationen, wie dieses Verfahren zur Prüfung von Substanzen unter gemäßigten Bedingungen anzuwenden ist.
Das Verfahren ist nicht auf flüchtige Substanzen anwendbar, d. h. Substanzen, bei denen H (Henry-Konstante) oder der Luft/Wasser-Verteilungskoeffizient größer als 1 ist oder bei denen der Dampfdruck 0,013 3 Pa bei 25 °C überschreitet.
ANMERKUNG Es kann nicht sichergestellt werden, dass die Prüfsubstanz während der gesamten Versuchsdauer stabil bleibt. Im Prüfverfahren wird die Persistenz der Prüfsubstanz nicht überwacht.
Qualité du sol - Inhibition de la reproduction de Collembola (Folsomia candida) par des contaminants du sol (ISO 11267:2014)
L'ISO 11267:2014 spécifie l'une des méthodes permettant d'évaluer la fonction d'habitat des sols et de déterminer les effets de contaminants du sol et de substances sur la reproduction de Folsomia candida Willem par absorption cutanée et ingestion. Cet essai chronique s'applique aux sols et matériaux du sol de qualité inconnue, par exemple provenant de sites contaminés, de sols amendés, de sols après remédiation, de sites industriels, agricoles ou d'autres sites d'intérêt et de déchets.
Les effets des substances sont évalués à l'aide d'un sol standard, de préférence un substrat de sol artificiel défini. Pour les sols contaminés, les effets sont déterminés dans le sol soumis à essai et dans un sol témoin. Selon l'objectif de l'étude, il convient que le substrat témoin et de dilution (gamme de dilutions d'un sol contaminé) soit un sol non contaminé comparable au sol à évaluer (sol de référence) ou un sol standard (par exemple un sol artificiel).
L'ISO 11267:2014 fournit des informations sur la manière d'utiliser cette méthode pour évaluer des substances dans des conditions tempérées.
La méthode ne s'applique pas aux substances volatiles, c'est-à-dire aux substances pour lesquelles H (constante de Henry) ou le coefficient de partage air/eau est supérieur à 1, ou pour lesquelles la pression de vapeur excède 0,013 3 Pa à 25 °C.
Kakovost tal - Zaviranje razmnoževanja vrste Folsomia candida iz rodu skakačev (Collembola) zaradi onesnaževal v tleh (ISO 11267:2014)
Standard EN-ISO 11267 določa eno od metod za ocenjevanje funkcije tal habitata in določevanje učinkov onesnaževal tal in snovi na razmnoževanje vrste Folsomia candida iz rodu skakačev (Collembola) z dermalnim ali prehranskim vnosom. Ta kronični preskus se uporablja za tla in talne materiale neznane kakovosti, na primer tla iz onesnaženih območij, obogatena tla, tla po sanaciji, industrijska, kmetijska in druga problematična mesta ter odpadne materiale. Vpliv snovi se določi z uporabo standardnih tal, najbolje z določenim umetnim talnim substratom. Za kontaminirana tla se učinki določijo v preskušenih in kontrolnih tleh. Glede na cilj raziskave sta kontrolni in zredčeni substrat (serija redčenja kontaminiranih tal) neokužena tla, primerljiva s tlemi, ki se jih preskuša (referenčna tla), ali standardna tla (npr. umetna tla). Ta mednarodni standard ponuja informacije o uporabi te metode za preskušanje snovi pod zmernimi pogoji. Ta metoda ni primerna za hlapljive snovi, npr. snovi, pri katerih je H (Henryjeva konstanta) ali koeficient deleža zraka/vode, večji od 1, ali pri katerih parni tlak pri 25 °C presega 0,013 3 Pa.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2014
.DNRYRVWWDO=DYLUDQMHUD]PQRåHYDQMDYUVWH)ROVRPLDFDQGLGDL]URGXVNDNDþHY
&ROOHPEROD ]DUDGLRQHVQDåHYDOYWOHK ,62
Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil
contaminants (ISO 11267:2014)
Bodenbeschaffenheit - Hemmung der Reproduktion von Collembolen (Folsomia candida)
durch Verunreinigungen (ISO 11267:2014)
Qualité du sol - Inhibition de la reproduction de Collembola (Folsomia candida) par des
contaminants du sol (ISO 11267:2014)
Ta slovenski standard je istoveten z: EN ISO 11267:2014
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11267
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2014
ICS 13.080.30
English Version
Soil quality - Inhibition of reproduction of Collembola (Folsomia
candida) by soil contaminants (ISO 11267:2014)
Qualité du sol - Inhibition de la reproduction de Collembola Bodenbeschaffenheit - Hemmung der Reproduktion von
(Folsomia candida) par des contaminants du sol (ISO Collembolen (Folsomia candida) durch Verunreinigungen
11267:2014) (ISO 11267:2014)
This European Standard was approved by CEN on 4 January 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
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© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11267:2014 E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 11267:2014) has been prepared by Technical Committee ISO/TC 190 “Soil quality” in
collaboration with Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held
by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2014, and conflicting national standards shall be withdrawn at
the latest by August 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11267:2014 has been approved by CEN as EN ISO 11267:2014 without any modification.
INTERNATIONAL ISO
STANDARD 11267
Second edition
2014-02-15
Soil quality — Inhibition of
reproduction of Collembola (Folsomia
candida) by soil contaminants
Qualité du sol — Inhibition de la reproduction de Collembola
(Folsomia candida) par des contaminants du sol
Reference number
ISO 11267:2014(E)
©
ISO 2014
ISO 11267:2014(E)
© ISO 2014
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ii © ISO 2014 – All rights reserved
ISO 11267:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents and material . 4
6 Apparatus . 6
7 Procedure. 6
7.1 Experimental design . 6
7.2 Preparation of test mixture . 7
7.3 Addition of the biological material . 9
7.4 Test conditions and measurements . 9
7.5 Determination of surviving Collembola . 9
8 Calculation and expression of results . 9
8.1 Calculation . 9
8.2 Expression of results . 9
9 Validity of the test .10
10 Statistical analysis .10
10.1 General .10
10.2 Single-concentration tests .10
10.3 Multi-concentration tests.11
11 Test report .11
Annex A (informative) Techniques for rearing and breeding of Collembola .13
Annex B (informative) Determination of water-holding capacity .15
Annex C (informative) Guidance on adjustment of pH of artificial soil .16
Annex D (informative) Extraction and counting of Collembola .17
Bibliography .18
ISO 11267:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
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For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
This second edition cancels and replaces the first edition (ISO 11267:1999), which has been technically
revised.
iv © ISO 2014 – All rights reserved
ISO 11267:2014(E)
Introduction
Ecotoxicological test systems are applied to obtain information about the effects of contaminants in soil
and are proposed to complement conventional chemical analysis (see [2] and [4]). Reference [2] includes
a list and short characterization of recommended and standardized test systems and [4] gives guidance
on the choice and evaluation of the bioassays. Aquatic test systems with soil eluate are applied to obtain
information about the fraction of contaminants potentially reaching the groundwater by the water path
(retention function of soils), whereas terrestrial test systems are used to assess the habitat function of
soils.
Soil-dwelling Collembola are ecologically relevant species for ecotoxicological testing. Springtails are
prey animals for a variety of endogeic and epigeic invertebrates and they contribute to decomposition
processes in soils. In acidic soils they may be the most important soil invertebrates besides enchytraeids
[19]
with respect to that function, since earthw
...
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