EN ISO 11290-1:2017

SLOVENSKI STANDARD
01-september-2017
1DGRPHãþD
SIST EN ISO 11290-1:1997
SIST EN ISO 11290-1:1997/A1:2005
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti in števila Listeria monocytogenes in Listeria spp. - 1. del: Metoda za
ugotavljanje prisotnosti (ISO 11290-1:2017)
Microbiology of the food chain - Horizontal method for the detection and enumeration of
Listeria monocytogenes and of Listeria spp. - Part 1: Detection method (ISO 11290-
1:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die
Zählung von Listeria monocytogenes und von Listeria spp. - Teil 1: Nachweisverfahren
(ISO 11290-1:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le
dénombrement de Listeria monocytogenes et de Listeria spp. - Partie 1: Méthode de
recherche (ISO 11290-1:2017)
Ta slovenski standard je istoveten z: EN ISO 11290-1:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11290-1
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 11290-1:1996
English Version
Microbiology of the food chain - Horizontal method for the
detection and enumeration of Listeria monocytogenes and
of Listeria spp. - Part 1: Detection method (ISO 11290-
1:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche et le dénombrement de Verfahren für den Nachweis und die Zählung von
Listeria monocytogenes et de Listeria spp. - Partie 1: Listeria monocytogenes und von Listeria spp. - Teil 1:
Méthode de recherche (ISO 11290-1:2017) Nachweisverfahren (ISO 11290-1:2017)
This European Standard was approved by CEN on 6 April 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11290-1:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11290-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2017, and conflicting national standards
shall be withdrawn at the latest by December 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11290-1:1996.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11290-1:2017 has been approved by CEN as EN ISO 11290-1:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 11290-1
Second edition
2017-05
Microbiology of the food chain —
Horizontal method for the detection
and enumeration of Listeria
monocytogenes and of Listeria spp. —
Part 1:
Detection method
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Listeria monocytogenes et de
Listeria spp. —
Partie 1: Méthode de recherche
Reference number
ISO 11290-1:2017(E)
©
ISO 2017
ISO 11290-1:2017(E)
© ISO 2017, Published in Switzerland
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ii © ISO 2017 – All rights reserved

ISO 11290-1:2017(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 2
4.1 General . 2
4.2 Primary enrichment in a selective liquid enrichment medium with reduced
concentration of selective agents (half-Fraser broth) . . 2
4.3 Secondary enrichment with a selective liquid enrichment medium with full
concentration of selective agents (Fraser broth) . 3
4.4 Plating out and identification . 3
4.5 Confirmation . 3
5 Culture media and reagents . 3
6 Equipment and consumables . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure. 4
9.1 Test portion and initial suspension . 4
9.2 Primary enrichment . 4
9.3 Secondary enrichment . 5
9.4 Plating out and identification . 5
9.4.1 General . 5
9.4.2 Agar Listeria according to Ottaviani and Agosti . 5
9.4.3 Second selective medium . 6
9.5 Confirmation of Listeria monocytogenes or Listeria spp. . 6
9.5.1 Selection of colonies for confirmation . 6
9.5.2 Confirmation of L. monocytogenes .  . 6
9.5.3 Confirmation of Listeria spp. . 10
9.6 Interpretation of morphological and physiological properties and of the
biochemical reactions .11
9.7 Additional characterization of isolated strains (optional) .11
10 Expression of results .11
11 Performance characteristics of the method .11
11.1 Method validation studies .11
11.2 Sensitivity .11
11.3 Specificity .11
11.4 Level of detection (LOD ) .11
12 Test report .12
13 Quality assurance .12
Annex A (normative) Diagram of procedure .13
Annex B (normative) Composition and preparation of culture media and reagents .14
Annex C (informative) Distinction of Listeria spp. from other genera .27
Annex D (informative) Reactions for the identification of Listeria species .28
Annex
...