EN ISO 22964:2017

SLOVENSKI STANDARD
01-julij-2017
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti Cronobacter spp. (ISO 22964:2017)
Microbiology in the food chain - Horizontal method for the detection of Cronobacter spp.
(ISO 22964:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis von
Cronobacter spp. (ISO 22964:2017)
Microbiologie de la chaine alimentaire - Méthode horizontale pour la détection de
Cronobacter spp. (ISO 22964:2017)
Ta slovenski standard je istoveten z: EN ISO 22964:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 22964
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2017
EUROPÄISCHE NORM
ICS 67.100.01; 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
detection of Cronobacter spp. (ISO 22964:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche de Cronobacter spp. (ISO Verfahren zum Nachweis von Cronobacter spp. (ISO
22964:2017) 22964:2017)
This European Standard was approved by CEN on 13 March 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22964:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 22964:2017) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall
be withdrawn at the latest by October 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 22964:2017 has been approved by CEN as EN ISO 22964:2017 without any modification.

INTERNATIONAL ISO
STANDARD 22964
First edition
2017-04
Microbiology of the food chain —
Horizontal method for the detection
of Cronobacter spp.
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche de Cronobacter spp.
Reference number
ISO 22964:2017(E)
©
ISO 2017
ISO 22964:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
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ii © ISO 2017 – All rights reserved

ISO 22964:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 Principle . 2
5.1 Non-selective pre-enrichment in BPW . 2
5.2 Enrichment in a selective medium (CSB) . 2
5.3 Plating out and identification on chromogenic agar (CCI agar) . 2
5.4 Confirmation . 2
6 Culture media and reagents . 2
7 Equipment and consumables . 2
8 Sampling . 3
9 Preparation of test sample . 3
10 Procedure (as shown in Annex A) . 3
10.1 Test portion . 3
10.2 Pre-enrichment . 4
10.3 Enrichment . 4
10.4 Isolation of presumptive Cronobacter spp. 4
10.5 Confirmation . 4
10.5.1 General. 4
10.5.2 Purification of colonies . . 5
10.5.3 Biochemical confirmation . 5
10.6 Interpretation of biochemical results. 6
11 Expression of results . 6
12 Performance characteristics of the method . 7
12.1 Interlaboratory study . 7
12.2 Sensitivity . 7
12.3 Specificity . 7
12.4 LOD .
50 7
13 Test report . 7
Annex A (normative) Diagram of the test procedure . 8
Annex B (normative) Composition, preparation and performance testing of culture media
and reagents . 9
Annex C (informative) Distinction of Cronobacter spp. from other genera .17
Annex D (informative) Method validation studies and performance characteristics .19
Bibliography .22
ISO 22964:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
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Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified du
...