ASTM E1093-91(1996)

NOTICE: This standard has either been superseded and replaced by a new version or discontinued.NOTICE: This standard has either been superseded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.Contact ASTM International (www.astm.org) for the latest information.
Designation: E 1093 – 91 (Reapproved 1996)
AMERICAN SOCIETY FOR TESTING AND MATERIALS
100 Barr Harbor Dr., West Conshohocken, PA 19428
Reprinted from the Annual Book of ASTM Standards. Copyright ASTM
Standard Specification for
Glass Prothrombin Pipet, Disposable
This standard is issued under the fixed designation E 1093; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 6. Physical Properties
1.1 This specification covers a glass disposable Prothrombin 6.1 Design—The Prothrombin pipet shall be made of one
pipet suitable for use in micro techniques for estimation of piece construction glass tubing that is straight and with uniform
Prothrombin time. bore and lightly firepolished on both ends. The pipet shall be
1.2 This precautionary statement pertains only to the test made to the dimensions as specified in Fig. X1.1.
method portion, Section 8, of this specification. This standard 6.2 Dimensions—The pipet shall be made of tubing with a
does not purport to address all of the safety concerns, if any, minimum outside diameter (o.d.) of 2.3 mm with an inside
associated with its use. It is the responsibility of the user of this diameter (i.d.) of 1.7 mm. The uniformity of the bore shall be
standard to establish appropriate safety and health practices 60.05 mm throughout the straight portion of the pipet. The
and determine the applicability of regulatory limitations prior pipet shall be a minimum of 160 mm long.
to use. 6.3 Capacity—The pipet shall be calibrated “to deliver”
(T.D.) 0.1 and 0.2 mL at 20°C. Marking shall be as specified in
2. Referenced Documents
6.5.
2.1 ASTM Standards: 6.3.1 Accuracy (see 3.1)—The accuracy from stated volume
E 438 Specification for Glasses in Laboratory Apparatus
shall be 62.0 % for the 0.1 and 0.2-mL capacity and shall be
2.2 ISO Standard: determined as specified in 8.1.
1769 Laboratory Glassware—Pipettes—Color Coding
6.3.2 Coeffıcient of Variation (see 3.2)—The coefficient of
variation from stated volume for the 0.1 and 0.2-mL capacity
3. Terminology
shall not exceed 2.0 % and shall be determined as specified in
3.1 Definitions of Terms Specific to This Standard:
8.1.
3.1.1 accuracy—the expected distribution of mean volumes
6.4 Graduation Lines—The pipet shall be calibrated and
around the stated volume.
marked with graduation lines at 0.1 and 0.2 mL from the tip of
3.1.2 coeffıcient of variation—the expected distribution of
the pipet. The graduation lines shall be 0.3 6 0.1 mm and shall
individual volumes around the mean volume.
completely encircle the tube.
3.1.3 disposable—Prothrombin pipets which are intended to
6.5 Pipet Nomenclature—The pipet shall be marked with
be used once only and then discarded.
0.1 and 0.2-mL markings slightly above the graduation lines.
The pipet may be marked with the inscription T.D. 20°C, or the
NOTE 1—Such pipets will only be expected to provide their specified
manufacturer’s or vendor’s name or trademark, or both.
performance during the original operation.
6.6 Blow-out Delivery—The Prothrombin pipet is designed
4. Classification
as a dual delivery system for the determinations of coagulation
assays. When utilizing the 0.2 mL calibration line, the tip of the
4.1 This specification covers only one glass pipet as illus-
pipet should contact the wall of the receiving vessel and allow
trated in Fig. X1.1.
the pipet to drain freely. The remaining quantity of liquid is to
5. Materials and Manufacture
be blown-out into the center of the receiving vessel. When
utilizing the 0.1 mL calibration line the contents are to be
5.1 The pipet shall be made of borosilicate glass, Type 1;
vigorously blown-out into the center of the receiving vessel
Class B, or soda lime glass, Type 2, in accordance with
with one quick effort. Either one band or two narrow bands
Specification E 438.
shall encircle the top end of the pipet to signify the contents
must be blown-out as shown in Fig. X1.1.
This specification is under the jurisdiction of ASTM Committee E-41 on
6.7 Color Coding—The pipet shall be color coded for
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.07 on
capacity as specified in ISO Standard 1769 with an “orange”
Microchemical Apparatus.
color band that is 6 6 2 mm wide and located 16 6 2 mm from
Current edition approved Aug. 15, 1991. Published October 1991. Originally
published as E 1093 – 86. Last previous edition E 1093 – 86.
the top of the pipet as shown in Fig. X1.1.
Annual Book of ASTM Standards, Vol 14.02.
6.8 Markings Permanency—Graduation lines, inscriptions,
Available from ISO, 1 Rue de Varemb
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