ASTM E1093-91(2000)e1

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e1
Designation: E 1093 – 91 (Reapproved 2000)
Standard Specification for
Glass Prothrombin Pipet, Disposable
This standard is issued under the fixed designation E 1093; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
e NOTE—Keywords were added editorially in February 2001.
1. Scope 5. Materials and Manufacture
1.1 ThisspecificationcoversaglassdisposableProthrombin 5.1 The pipet shall be made of borosilicate glass, Type 1;
pipet suitable for use in micro techniques for estimation of Class B, or soda lime glass, Type 2, in accordance with
Prothrombin time. Specification E 438.
1.2 This precautionary statement pertains only to the test
6. Physical Properties
method portion, Section 8, of this specification. This standard
6.1 Design—The Prothrombin pipet shall be made of one
does not purport to address all of the safety concerns, if any,
associated with its use. It is the responsibility of the user of this piececonstructionglasstubingthatisstraightandwithuniform
bore and lightly firepolished on both ends. The pipet shall be
standard to establish appropriate safety and health practices
and determine the applicability of regulatory limitations prior made to the dimensions as specified in Fig. X1.1.
6.2 Dimensions—The pipet shall be made of tubing with a
to use.
minimum outside diameter (o.d.) of 2.3 mm with an inside
2. Referenced Documents
diameter (i.d.) of 1.7 mm. The uniformity of the bore shall be
2.1 ASTM Standards: 60.05 mm throughout the straight portion of the pipet. The
E 438 Specification for Glasses in Laboratory Apparatus pipet shall be a minimum of 160 mm long.
2.2 ISO Standard: 6.3 Capacity—The pipet shall be calibrated “to deliver”
1769 Laboratory Glassware—Pipettes—Color Coding (T.D.) 0.1 and 0.2 mLat 20°C. Marking shall be as specified in
6.5.
3. Terminology
6.3.1 Accuracy(see3.1)—Theaccuracyfromstatedvolume
3.1 Definitions of Terms Specific to This Standard: shall be 62.0 % for the 0.1 and 0.2-mL capacity and shall be
3.1.1 accuracy—the expected distribution of mean volumes
determined as specified in 8.1.
around the stated volume. 6.3.2 Coeffıcient of Variation (see 3.2)—The coefficient of
3.1.2 coeffıcient of variation—the expected distribution of
variation from stated volume for the 0.1 and 0.2-mL capacity
individual volumes around the mean volume. shall not exceed 2.0 % and shall be determined as specified in
3.1.3 disposable—Prothrombinpipetswhichareintendedto
8.1.
be used once only and then discarded. 6.4 Graduation Lines—The pipet shall be calibrated and
marked with graduation lines at 0.1 and 0.2 mLfrom the tip of
NOTE 1—Such pipets will only be expected to provide their specified
the pipet.The graduation lines shall be 0.3 6 0.1 mm and shall
performance during the original operation.
completely encircle the tube.
4. Classification
6.5 Pipet Nomenclature—The pipet shall be marked with
0.1 and 0.2-mL markings slightly above the graduation lines.
4.1 This specification covers only one glass pipet as illus-
ThepipetmaybemarkedwiththeinscriptionT.D.20°C,orthe
trated in Fig. X1.1.
manufacturer’s or vendor’s name or trademark, or both.
6.6 Blow-out Delivery—The Prothrombin pipet is designed
as a dual delivery system for the determinations of coagulation
This specification is under the jurisdiction of ASTM Committee E41 on
assays.Whenutilizingthe0.2mLcalibrationline,thetipofthe
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.07 on
pipet should contact the wall of the receiving vessel and allow
Microchemical Apparatus.
Current edition approved Aug. 15, 1991. Published October 1991. Originally
the pipet to drain freely. The remaining quantity of liquid is to
published as E 1093 – 86. Last previous edition E 1093 – 86.
be blown-out into the center of the receiving vessel. When
Annual Book of ASTM Standards, Vol 14.04.
utilizing the 0.1 mL calibration line the contents are to be
Available from ISO, 1 Rue de Varembe, Case Postal 56, Crt 1221, Geneva 20,
Switzerland. vigorously blown-out into the center of the receiving vessel
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E 1093
with one quick effort. Either one band or two narrow bands
W = apparent mass of liquid (water), weighed in air, and
shall encircle the top end of the pipet to signify the contents
Z = apparent specific volume of liquid (water).
must be blown-out as shown in Fig. X1.1.
Values of Z for water are given in the appendix.
6.7 Color Coding—The pipet shall be color coded for
8.3 Capacity Deviation (single pipet)—In accordance with
capacity as specified in ISO Standard 1769 with an “orange”
the methods outlined, capacity deviation is the difference
colorbandthatis6 62m
...