ASTM E1241-22

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1241 − 22
Standard Guide for
1
Conducting Early Life-Stage Toxicity Tests with Fishes
This standard is issued under the fixed designation E1241; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
Section
1.1 Thisguidedescribesproceduresforobtaininglaboratory
Referenced Documents 2
data concerning the adverse effects of a test material added to Terminology 3
Summary of Standard 4
dilution water—but not to food—on certain species of fresh-
Significance and Use 5.1
waterandsaltwaterfishesduring28dayto120day(depending
Hazards 6
on species) continuous exposure, beginning before hatch and Apparatus 7
Facilities 7.1
ending after hatch, using flow-through exposures. This guide
Construction Materials 7.2
will probably be useful for conducting early life-stage toxicity
Metering System 7.3
Test Chambers and Incubation Cups 7.4
tests with some other species of fish, although modifications
Cleaning 7.5
might be necessary.
Acceptability 7.6
Dilution Water 8
1.2 Other modifications of these procedures might be justi-
Requirements 8.1
fied by special needs or circumstances.Although using appro-
Source 8.2
priate procedures is more important than following prescribed Treatment 8.3
Characterization 8.4
procedures,resultsoftestsconductedusingunusualprocedures
Test Material 9
are not likely to be comparable to results of many other tests.
General 9.1
Comparisonofresultsobtainedusingmodifiedandunmodified
Stock Solution 9.2
Test Concentration(s) 9.3
versions of these procedures might provide useful information
Test Organisms 10
concerning new concepts and procedures for conducting early
Species 10.1
life-stage toxicity tests with fishes.
Age 10.2
Source 10.3
1.3 These procedures are applicable to all chemicals, either
Brood Stock 10.4
individually or in formulations, commercial products, or Handling 10.5
Procedure 11
known mixtures, that can be measured accurately at the
Experimental Design 11.1
necessary concentrations in water. With appropriate modifica-
Dissolved Oxygen 11.2
Temperature 11.3
tions these procedures can be used to conduct tests on
Beginning the Test 11.4
temperature, dissolved oxygen, and pH and on such materials
Thinning 11.5
as aqueous effluents (see Guide E1192), leachates, oils, par-
Feeding 11.6
Duration of Test 11.7
ticulate matter, sediments, and surface waters.
Biological Data 11.8
1.4 This guide is arranged as follows: Other Measurements 11.9
Analytical Methodology 12
Acceptability of Test 13
Calculation of Results 14
Documentation 15
Appendixes
1
ThisguideisunderthejurisdictionofASTMCommitteeE50onEnvironmental Appendix X1 Salmon, Trout, and Char
Appendix X2 Northern pike
Assessment, Risk Management and CorrectiveAction and is the direct responsibil-
Appendix X3 Fathead minnow
ity of Subcommittee E50.47 on Biological Effects and Environmental Fate.
Appendix X4 White sucker
Current edition approved April 1, 2022. Published May 2022. Originally
Appendix X5 Channel catfish
approved in 1988. Last previous edition approved in 2013 as E1241–05(2013)
Appendix X6 Bluegill
which was withdrawn January 2022 and reinstated in April 2022. DOI: 10.1520/
Appendix X7 Gulf toadfish
E1241-22.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E1241 − 22
acceptability of the test (see 13.1). “Should” is used to state
Appendix X8 Sheepshead minnow
Appendix X9 Silversides
that the specified condition is recommended and ought to be
Appendix X10 Statistical Guidance
met if possible.Although violation of one “should” is rarely a
Appendix X11. Striped Bass
serious matter, violation of several will often render the results
questionable.Termssuchas“isdesirable,”“isoftendesirable,”
and “might be desirable” are used in connection with less
1.5 This standard does not purport to address all of the
importantfactors.“May”isusedtomean“is(are)allowedto,”
safety concerns, if any, associated with its use. It is the
“can”isusedtomean“is(are)ableto,”and“might”isusedto
responsibility of the user of this standard to establish appro-
mean “could possibly.” Thus the classic distinction between
priate safety, health, and environmental practices and deter-
“may” and “can” is preserved, and “might” is never used as a
mine the applicability of regulatory limitations prior to use.
synonym for either “may” or “can.”
Specific hazard statements are given in Section
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