ISO/TS 21872-2:2020

TECHNICAL ISO/TS
SPECIFICATION 21872-2
First edition
Microbiology of the food chain —
Horizontal method for the
determination of Vibrio spp. —
Part 2:
Enumeration of total and
potentially enteropathogenic Vibrio
parahaemolyticus in seafood using
nucleic acid hybridization
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la détermination des Vibrio spp. —
Partie 2: Dénombrement de Vibrio parahaemolyticus total et
potentiellement entéropathogène dans les fruits de mer, par
hybridation des acides nucléiques
PROOF/ÉPREUVE
Reference number
ISO/TS 21872-2:2020(E)
©
ISO 2020

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ISO/TS 21872-2:2020(E)

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Published in Switzerland
ii PROOF/ÉPREUVE © ISO 2020 – All rights reserved

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ISO/TS 21872-2:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Plating . 2
4.3 Preparation of membranes . 2
4.4 Hybridization. 2
4.5 Detection . 2
5 Culture media and reagents . 3
5.1 General . 3
5.2 Hybridization reagents . 3
5.2.1 Hybridization buffer . 3
5.2.2 Digoxigenin-labelled oligoprobes . 3
5.2.3 Control materials .
...