Water quality - Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna (ISO 16665:2014)

ISO 16665:2014 provides guidelines on the quantitative collection and processing of subtidal soft-bottom macrofaunal samples in marine waters.
ISO 16665:2014 encompasses: a) development of the sampling programme; b) requirements for sampling equipment; c) sampling and sample treatment in the field; d) sorting and species identification; e) storage of collected and processed material.
ISO 16665:2014 does not specifically address the following, although some elements may be applicable: bioassay sub-sampling; deep water (>750 m) or offshore sampling; in situ faunal studies, e.g. recolonization assays; non-benthic organisms caught in the sampling device; estuarine sampling; intertidal sampling; meiofaunal sampling and analysis; sampling by dredge and sledge; self-contained underwater breathing apparatus (SCUBA) sampling; statistical design.
Accuracy of position fixing is determined by the geographical area, equipment used and survey objective.

Wasserbeschaffenheit - Anleitung für die quantitative Probenahme und Probenbearbeitung mariner Weichboden-Makrofauna (ISO 16665:2014)

Diese Internationale Norm enthält Anleitungen zur quantitativen Sammlung und Bearbeitung von Proben der sublitoralen Weichboden-Makrofauna in marinen Gewässern.
Diese Internationale Norm umfasst:
a) Entwicklung des Probenahmeprogrammes;
b) Anforderungen an die Probenahmegeräte;
c) Probenahme und Probenbehandlung vor Ort;
d) Sortierverfahren und Artenbestimmung;
e) Aufbewahrung von gesammeltem und bearbeitetem Material.
Diese Internationale Norm geht auf folgende Aspekte nicht speziell ein, wenn auch einige Elemente im Rahmen von Untersuchungen auftreten könnten:
- Entnahme von Teilproben für Biotests;
- Probenahme in Tiefenwasser (> 750 m) oder küstenfernen Bereichen;
- faunistische In-situ-Untersuchungen, z. B. Wiederbesiedlungsuntersuchungen;
- nicht benthische Organismen, die mit dem Probenahmegerät gefangen werden;
- Probenahme in Ästuaren;
- Probenahme in Tidebereichen;
- Probenahme und Analyse der Meiofauna [9];
- Probenahme mit Dredgen und Schlitten;
- Probenahme mit Hilfe von Tauchgeräten (SCUBA-Tauchen);
- Konzept für eine statistische Auswertung.
Die Genauigkeit bei der Festlegung der Position ist durch das geographische Gebiet, die verwendete Ausrüstung und das Ziel der Untersuchung vorgegeben.

Qualité de l'eau - Lignes directrices pour l'échantillonnage quantitatif et le traitement d'échantillons de la macrofaune marine des fonds meubles (ISO 16665:2014)

L'ISO 16665:2014 fournit des lignes directrices sur le prélèvement quantitatif et le traitement d'échantillons de la macrofaune des fonds meubles subtidaux en eaux marines.
L'ISO 16665:2014 englobe: a) le développement du programme d'échantillonnage; b) les exigences relatives à l'équipement nécessaire pour l'échantillonnage; c) l'échantillonnage et le traitement des échantillons sur le terrain; d) le tri et l'identification des espèces; e) le stockage des matériels collectés et traités.
L'ISO 16665:2014 ne traite pas spécifiquement de ce qui suit, bien qu'elle puisse s'appliquer à certains de ces éléments: le sous-échantillonnage pour les essais biologiques; l'échantillonnage en eaux profondes (>750 m) ou au large; les études faunistiques in situ, par exemple les essais de recolonisation; les organismes non benthiques capturés par le dispositif d'échantillonnage; l'échantillonnage en milieu estuarien; l'échantillonnage en zone intertidale; l'échantillonnage et l'analyse de la méiofaune; l'échantillonnage au moyen de dragues et de traîneaux; l'échantillonnage au moyen de scaphandres autonomes; la conception statistique.
L'exactitude de la détermination de la position dépend de la zone géographique, de l'équipement utilisé et de l'objectif de l'étude.

Kakovost vode - Smernice za kvantitativno vzorčenje in obdelavo vzorcev morske makrofavne sedimentnega dna (ISO 16665:2014)

General Information

Status
Published
Publication Date
17-Dec-2013
Withdrawal Date
29-Jun-2014
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
18-Dec-2013
Completion Date
18-Dec-2013

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SLOVENSKI STANDARD
01-marec-2014
1DGRPHãþD
SIST EN ISO 16665:2005
.DNRYRVWYRGH6PHUQLFH]DNYDQWLWDWLYQRY]RUþHQMHLQREGHODYRY]RUFHYPRUVNH
PDNURIDYQHVHGLPHQWQHJDGQD ,62
Water quality - Guidelines for quantitative sampling and sample processing of marine
soft-bottom macrofauna (ISO 16665:2014)
Wasserbeschaffenheit - Anleitung für die quantitative Probenahme und
Probenbearbeitung mariner Weichboden-Makrofauna (ISO 16665:2014)
Qualité de l'eau - Lignes directrices pour l'échantillonnage quantitatif et le traitement
d'échantillons de la macrofaune marine des fonds meubles (ISO 16665:2014)
Ta slovenski standard je istoveten z: EN ISO 16665:2013
ICS:
13.060.10 Voda iz naravnih virov Water of natural resources
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16665
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2013
ICS 13.060.70; 13.060.10 Supersedes EN ISO 16665:2005
English Version
Water quality - Guidelines for quantitative sampling and sample
processing of marine soft-bottom macrofauna (ISO 16665:2014)
Qualité de l'eau - Lignes directrices pour l'échantillonnage Wasserbeschaffenheit - Anleitung für die quantitative
quantitatif et le traitement d'échantillons de la macrofaune Probenahme und Probenbearbeitung mariner Weichboden-
marine des fonds meubles (ISO 16665:2014) Makrofauna (ISO 16665:2014)
This European Standard was approved by CEN on 6 December 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16665:2013 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 16665:2013) has been prepared by Technical Committee ISO/TC 147 “Water quality”
in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2014, and conflicting national standards shall be withdrawn at
the latest by June 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 16665:2005.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 16665:2014 has been approved by CEN as EN ISO 16665:2013 without any modification.

INTERNATIONAL ISO
STANDARD 16665
Second edition
2014-01-15
Water quality — Guidelines for
quantitative sampling and sample
processing of marine soft-bottom
macrofauna
Qualité de l’eau — Lignes directrices pour l’échantillonnage
quantitatif et le traitement d’échantillons de la macrofaune marine
des fonds meubles
Reference number
ISO 16665:2014(E)
©
ISO 2014
ISO 16665:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 16665:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Terms and definitions . 1
2.1 Ecological or biological concepts . 2
2.2 Surveys and samples . 2
3 Strategies and objectives for soft-bottom faunal surveys . 3
3.1 Sampling programme and plan . 3
3.2 Positioning of sampling stations. 3
3.3 Reference stations . 5
3.4 Types of survey . 5
3.5 Change in sampling programme and intercalibration . 8
4 Sampling . 8
4.1 Documentation and field log . 8
4.2 Sampling and sample processing in the field .10
4.3 Sample fixation .13
4.4 Background environmental descriptors .14
5 Sample processing in the laboratory .17
5.1 Sorting .17
5.2 Sample residue .18
6 Taxon determination and quantification .18
6.1 Level of identification and taxon lists . .18
6.2 Quantification .19
6.3 Reference collection .19
6.4 Biomass .20
6.5 Data reporting .20
6.6 Storage and archiving .20
7 Quality assurance and quality control .21
7.1 General .21
7.2 Auditing .21
7.3 Equipment calibration and operating safety.21
7.4 Training .22
7.5 Checklists, sample log and anomaly reporting .22
7.6 Quality of sample sorting .22
7.7 Quality of taxon identification .22
7.8 Identification literature .23
7.9 Reference and collection.23
Annex A (informative) Sampling devices .24
Annex B (informative) Processing of samples with live specimens .29
Annex C (informative) Biomass measurements .30
Annex D (informative) Processing particularly large samples .32
Bibliography .33
ISO 16665:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represente
...

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