EN ISO 16665:2005

SLOVENSKI STANDARD
01-december-2005
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Water quality - Guidelines for quantitative sampling and sample processing of marine
soft-bottom macrofauna (ISO 16665:2005)
Wasserbeschaffenheit - Anleitung für die quantitative Probenahme und
Probenbearbeitung mariner Weichboden-Makrofauna (ISO 16665:2005)
Qualité de l'eau - Lignes directrices pour l'échantillonnage quantitatif et le traitement
d'échantillons de la macrofaune marine des fonds meubles (ISO 16665:2005)
Ta slovenski standard je istoveten z: EN ISO 16665:2005
ICS:
13.060.10 Voda iz naravnih virov Water of natural resources
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16665
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2005
ICS 13.060.70; 13.060.10
English Version
Water quality - Guidelines for quantitative sampling and sample
processing of marine soft-bottom macrofauna (ISO 16665:2005)
Qualité de l'eau - Lignes directrices pour l'échantillonnage Wasserbeschaffenheit - Anleitung für die quantitative
quantitatif et le traitement d'échantillons de la macrofaune Probenahme und Probenbearbeitung mariner Weichboden-
marine des fonds meubles (ISO 16665:2005) Makrofauna (ISO 16665:2005)
This European Standard was approved by CEN on 7 April 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16665:2005: E
worldwide for CEN national Members.

Foreword
This document (EN ISO 16665:2005) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by April 2006, and conflicting national
standards shall be withdrawn at the latest by April 2006.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Endorsement notice
The text of ISO 16665:2005 has been approved by CEN as EN ISO 16665:2005 without any
modifications.
INTERNATIONAL ISO
STANDARD 16665
First edition
2005-10-15
Water quality — Guidelines for
quantitative sampling and sample
processing of marine soft-bottom
macrofauna
Qualité de l'eau — Lignes directrices pour l'échantillonnage quantitatif
et le traitement d'échantillons de la macrofaune marine des fonds
meubles
Reference number
ISO 16665:2005(E)
©
ISO 2005
ISO 16665:2005(E)
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ii © ISO 2005 – All rights reserved

ISO 16665:2005(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Terms and definitions. 2
3 Quality and safety. 3
3.1 Health and safety requirements . 3
3.2 Quality assurance and quality control. 4
4 Strategies and objectives for soft-bottom faunal surveys . 5
4.1 Sampling programme and plan . 5
4.2 Positioning of sampling stations . 5
4.3 Reference stations. 6
4.4 Types of surveys. 6
4.5 Change in sampling programme and intercalibration . 10
5 Sampling. 10
5.1 Documentation and field log . 10
5.2 Sampling and sample processing in the field . 11
5.3 Sample fixation . 15
5.4 Background environmental descriptors. 15
6 Sample processing in the laboratory. 18
6.1 Sorting . 18
6.2 Sample residue . 18
7 Taxon determination and quantification .19
7.1 Level of identification and taxon lists. 19
7.2 Quantification. 19
7.3 Reference collection (see also 7.7.8). 20
7.4 Biomass . 20
7.5 Data reporting . 20
7.6 Storage and archiving . 21
7.7 Analytical quality control and quality assurance . 21
Annex A (informative) Processing particularly large samples . 24
Annex B (informative) Sampling devices. 25
Annex C (informative) Biomass measurements. 27
Bibliography . 28

ISO 16665:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 16665 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
iv © ISO 2005 – All rights reserved

ISO 16665:2005(E)
Introduction
Analysis of macrofaunal communities in soft-bottom sediments is an integral part of marine environmental
assessment. The faunal composition, in terms of both the species present and their relative abundance,
reflects integrated environmental conditions in the survey area over a period of time. The composition and
structure of soft-bottom macrofaunal communities therefore can be used to characterise environmental
conditions and estimate the extent of environmental impact.
Characterisation of environmental conditions is usually based on quantitative methods, in this case by relating
the numbers of species and individuals captured to a known area of sea floor. For accurate data interpretation,
it is essential to add information on the geophysical/geochemical characteristics or properties of the water
masses and bottom sediments, including nutrients, oxygenation and redox state where appropriate.
For effective data utilisation and quality assurance of the work carried out, it is essential that surveys are
intercomparable temporally, spatially and between operators. This International Standard contributes to
on-going work on quality assurance of data from soft-bottom macrofaunal surveys. These guidelines primarily
aim assisting in standardising monitoring surveys carried out for commercial purposes or in connection with
the EU Water Framework Directive. For this reason, detailed specifications are given in areas of consequence
for data intercompatibility.
Where appropriate, cost-benefit issues have been taken into consideration, and accepted minimal
requirements for general environmental impact assessment have been given. The cited minimum
requirements for accuracy are not intended to satisfy research needs, or to provide a full ecological
understanding of the sampling area. Designers of programmes for research or other studies requiring a
detailed knowledge of soft-bottom macrofauna should consult the guidelines given in Reference [17] for
decisions of survey design and sampling frequency.
This International Standard applies to all areas of the sea floor where it is p
...