EN 14133:2009

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Ochratoxin A in Wein und Bier - HPLC-Verfahren mit Reinigung an einer ImmunoaffinitätssäuleProduits alimentaires - Dosage de l'ochratoxine A dans le vin et la bière - Méthode par purification sur colonne d'immuno-affinité suivie d'une analyse par chromatographie liquide haute performance (CLHP)Foodstuffs - Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up67.160.10Alcoholic beveragesICS:Ta slovenski standard je istoveten z:EN 14133:2009SIST EN 14133:2009en,fr,de01-december-2009SIST EN 14133:2009SLOVENSKI
STANDARDSIST EN 14133:2003/AC:2007SIST EN 14133:20031DGRPHãþD

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14133May 2009ICS 67.160.10Supersedes EN 14133:2003
English VersionFoodstuffs - Determination of ochratoxin A in wine and beer -HPLC method with immunoaffinity column clean-upProduits alimentaires - Dosage de l'ochratoxine A dans levin et la bière - Méthode par purification sur colonned'immuno-affinité suivie d'une analyse par chromatographieliquide haute performance (CLHP)Lebensmittel - Bestimmung von Ochratoxin A in Wein undBier - HPLC-Verfahren mit Reinigung an einerImmunoaffinitätssäuleThis European Standard was approved by CEN on 24 May 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
Avenue Marnix 17,
B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14133:2009: ESIST EN 14133:2009

Precision data . 11 Bibliography . 13
Pipette 0,5 ml of the 2 µg/ml ochratoxin A standard solution (4.15) into a 10 ml volumetric flask (5.3) and evaporate the solvent under a stream of nitrogen. Redissolve in 10 ml of mobile phase (4.11). This gives a mass concentration of 100 ng/ml ochratoxin A.
Prepare six HPLC calibrants in separate 5 ml volumetric flasks (5.3) according to Table 1. Dilute each solution to 5 ml with HPLC mobile phase (4.11). SIST EN 14133:2009

Table 1 — Preparation of calibration solutions
Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 µl of filtered mobile phase (4.11) 4970 4900 4700 4000 3000 2000 µl of 100 ng/ml OTA solution
30 100 300 1000 2000 3000 OTA mass concentration (ng/ml) 0,6 2,0 6,0 20 40 60 injected ng OTA
0,06 0,20 0,60 2,00 4,00 6,00
Prepare the calibration solutions at the beginning of every day of the analysis.
4.17 Immunoaffinity columns The immunoaffinity column shall contain antibodies raised against ochratoxin A. The column shall have a total capacity of not less than 100 ng of ochratoxin A and shall give a recovery of not less than 85 % when a diluted wine solution containing 100 ng of ochratoxin A is applied.
5 Apparatus Usual laboratory equipment and, in particular, the following: 5.1 Microbalance, capable to measure 0,01 mg 5.2 Glass vials, approximately 4 ml, with polytetrafluoroethylene (PTFE)-lined screw cap, or appropriate sealable screw cap. Certain types of vials can lead to losses of ochratoxin A during evaporation. To avoid this, silanization can be applied. Prepare vials by filling them with silanizing reagent and leave this reagent in the vial for 1 min. Rinse the vial twice with appropriate solvent (toluene, acetone or hexane) followed by water (twice) and dry the vial. 5.3 Volumetric flasks, 5 ml and 10 ml volume 5.4 Vacuum manifold, to accommodate immunoaffinity columns 5.5 Reservoirs and attachments, to fit to immunoaffinity columns 5.6 Glass microfibre filters, pore size 1,6 µm, (e.g. Whatman GF/A1 or equivalent) 5.7 Solvent evaporator 5.8 Calibrated microliter syringe(s) or microliter pipette(s) 5.9 HPLC apparatus consisting of
1 Whatman is an example of a suitable product available commercially. This information is given for the convenience of users of this European Standard and does not constitute an endorsement by CEN of these products. Equivalent products can be used if they can be shown to give equivalent results. SIST EN 14133:2009

Connect the immunoaffinity column (4.17) to the vacuum manifold (5.4) and attach the reservoir (5.5) to the immunoaffinity column. Add 10 ml (equivalent to 5 ml wine or beer) of the diluted
...