EN ISO 15952:2018
(Main)Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2018)
Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2018)
This document specifies a semi-static method for determining the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added:
- substances, mixtures or preparations;
- soils (contaminated or of unknown quality) or waste materials.
This test takes into account the possible changes in the test substance, preparation, soil or waste material because the test mixtures are prepared and renewed every week during the 28-day test period.
A static method may be implemented in addition to the semi-static method (optional). This method is described in Annex A.
This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C.
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken (Helicidae) - Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO 15952:2018)
Diese Internationale Norm legt ein semistatisches (halbkontinuierliches) Verfahren zur Bestimmung der Wirkungen von Kontaminanten auf das Wachstum und Überleben von jungen Schnecken, üblicherweise Helix aspersa aspersa Müller (Cornu aspersum), fest. Die Tiere werden unter Verwendung eines Prüfsubstrats (künstlicher oder natürlicher Boden in Abhängigkeit von der Zielsetzung der Studie), dem festgelegte Mengen von Folgendem zugesetzt werden, über den Haut und Verdauungspfad exponiert:
Substanzen, Mischungen oder Zubereitungen;
Böden (verunreinigte (kontaminierte) oder von unbekannter Beschaffenheit) oder Abfälle.
Diese Prüfung berücksichtigt mögliche Veränderungen der Prüfsubstanz, der Vorbereitung, des Bodens oder Abfalls, weil im Verlauf der 28 tägigen Prüfdauer die Prüfmischungen jede Woche hergestellt und erneuert werden.
Zusätzlich zu dem semistatischen Verfahren kann ein statisches Verfahren angewendet werden (optional). Dieses Verfahren ist in Anhang A beschrieben.
Dieses Verfahren gilt nicht für Substanzen, bei denen der Luft/Boden Verteilungskoeffizient größer als eins ist, oder für Substanzen mit einem Dampfdruck, der 300 Pa bei 25 °C übersteigt.
Qualité du sol - Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) - Détermination des effets sur la croissance par contamination du sol (ISO 15952:2018)
Le présent document s'applique à une méthode semi-statique pour la détermination des effets de contaminants sur la croissance et la survie d'escargots juvéniles, généralement Helix aspersa aspersa Müller. Les animaux sont exposés par les voies cutanée et digestive à un substrat d'essai (sol artificiel ou naturel selon l'objectif de l'étude) auquel sont ajoutées des quantités définies:
- de substances, de mélanges ou de préparations;
- de sols (contaminés ou de qualité inconnue) ou de déchets.
Cet essai prend en considération les changements éventuels de la substance d'essai, de la préparation, du sol ou des déchets, étant donné que les mélanges d'essai sont préparés et renouvelés chaque semaine pendant la période d'essai de 28 jours.
Il est admis de mettre en œuvre une méthode statique en sus de la méthode semi-statique (facultatif). Cette méthode est décrite dans l'Annexe A.
Cette méthode ne s'applique pas aux substances dont le coefficient de partage air/sol est supérieur à 1, ou pour lesquelles la pression de vapeur est supérieure à 300 Pa à 25 °C.
Kakovost tal - Vpliv onesnaževal na juvenilne (mladostniške) stadije kopenskih polžev (Helicidae) - Ugotavljanje vplivov na rast zaradi onesnaženja tal (ISO 15952:2018)
Ta dokument določa polstatično metodo za določevanje učinkov onesnaževal na rast in preživetje mladih polžev, običajno vrste Helix aspersa aspersa Müller. Živali so izpostavljene preskusnemu substratu po kožni in prebavni poti (umetna ali naravna zemlja glede na cilj študije), ki so mu so dodane opredeljene količine naslednjega:
– snovi, mešanic ali preparatov;
– zemljin (onesnažene ali neznane kakovosti) ali odpadnih materialov.
Ta preskus upošteva možne spremembe v preskusni snovi, pripravku, zemljini ali odpadnemu materialu, saj se preskusne mešanice pripravljajo in obnavljajo vsak teden v 28-dnevnem preskusnem obdobju. Poleg polstatične metode je mogoče uporabiti tudi statično metodo (izbirno). Ta metoda je opisana v dodatku A.
Ta metoda ni primerna za snovi, pri katerih je koeficient deleža zraka/zemljine večji od ena, ali snovi, pri katerih parni tlak pri 25 °C presega 300 Pa.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2018
1DGRPHãþD
SIST EN ISO 15952:2012
Kakovost tal - Vpliv onesnaževal na juvenilne (mladostniške) stadije kopenskih
polžev (Helicidae) - Ugotavljanje vplivov na rast zaradi onesnaženja tal (ISO
15952:2018)
Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of
the effects on growth by soil contamination (ISO 15952:2018)
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken -
Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO
15952:2018)
Qualité du sol - Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) -
Détermination des effets sur la croissance par contamination du sol (ISO 15952:2018)
Ta slovenski standard je istoveten z: EN ISO 15952:2018
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 15952
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2018
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 15952:2011
English Version
Soil quality - Effects of pollutants on juvenile land snails
(Helicidae) - Determination of the effects on growth by soil
contamination (ISO 15952:2018)
Qualité du sol - Effets des polluants vis-à-vis des Bodenbeschaffenheit - Wirkungen von Schadstoffen
escargots juvéniles (Helicidae) - Détermination des auf Jungtiere von Landschnecken - Bestimmung der
effets sur la croissance par contamination du sol (ISO Wirkungen auf das Wachstum durch
15952:2018) Bodenverunreinigung (ISO 15952:2018)
This European Standard was approved by CEN on 15 June 2018.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15952:2018 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 15952:2018) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2019, and conflicting national standards shall
be withdrawn at the latest by January 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 15952:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 15952:2018 has been approved by CEN as EN ISO 15952:2018 without any modification.
INTERNATIONAL ISO
STANDARD 15952
Second edition
2018-06
Soil quality — Effects of pollutants
on juvenile land snails (Helicidae) —
Determination of the effects on growth
by soil contamination
Qualité du sol — Effets des polluants vis-à-vis des escargots juvéniles
(Helicidae) — Détermination des effets sur la croissance par
contamination du sol
Reference number
ISO 15952:2018(E)
©
ISO 2018
ISO 15952:2018(E)
© ISO 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
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ii © ISO 2018 – All rights reserved
ISO 15952:2018(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Test environment . 3
6 Reagents . 3
7 Apparatus . 5
8 Storage and preparation of the samples . 6
8.1 Soil to be tested . 6
8.2 Waste material . 6
9 Procedure. 6
9.1 Preparation of the test . 6
9.1.1 Selection of the concentrations to be tested . 6
9.1.2 Preparation of test mixtures . 7
9.2 Distribution of test mixture . 8
9.3 Introduction of the feed. 8
9.4 Introduction of the biological reagent . 8
9.5 Handling during the tests . 8
9.5.1 General. 8
9.5.2 Routine care . 9
9.5.3 Weekly task . 9
10 Reference substance .10
11 Calculations and expression of results .10
11.1 Calculations .10
11.2 Expression of results .12
12 Validity of test .13
13 Test report .13
Annex A (informative) Static method .15
Annex B (informative) Breeding technique for snails .16
Annex C (informative) Example of composition of snail feed .21
Annex D (informative) Example of table of data .22
Annex E (informative) Example of results with Helix aspersa aspersa .23
Annex F (informative) Determination of the effects on growth by food contamination .27
Annex G (informative) Suitable other snail species .30
Bibliography .31
ISO 15952:2018(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different typ
...
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