ISO 7251:2005/Amd 1:2023
(Amendment)Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of presumptive Escherichia coli - Most probable number technique - Amendment 1: Inclusion of performance testing of culture media and reagents
Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of presumptive Escherichia coli - Most probable number technique - Amendment 1: Inclusion of performance testing of culture media and reagents
Microbiologie des aliments — Méthode horizontale pour la recherche et le dénombrement d'Escherichia coli présumés — Technique du nombre le plus probable — Amendment 1: Inclusion des essais de performance des milieux de culture et réactifs
General Information
- Status
- Published
- Publication Date
- 11-Oct-2023
- Technical Committee
- ISO/TC 34/SC 9 - Microbiology
- Drafting Committee
- ISO/TC 34/SC 9 - Microbiology
- Current Stage
- 6060 - International Standard published
- Start Date
- 12-Oct-2023
- Due Date
- 16-Jun-2023
- Completion Date
- 12-Oct-2023
Relations
- Effective Date
- 06-Jun-2022
Overview
ISO 7251:2005/Amd 1:2023 is an essential international standard issued by the International Organization for Standardization (ISO) that focuses on the microbiological analysis of food and animal feeding stuffs. This amendment to the 2005 third edition standard specifically enhances the horizontal method for detecting and enumerating presumptive Escherichia coli using the Most Probable Number (MPN) technique. The 2023 amendment introduces performance testing requirements for culture media and reagents, improving the reliability and accuracy of microbiological testing in laboratories worldwide.
Key Topics
Scope and Purpose
ISO 7251:2005/Amd 1:2023 standardizes the detection and enumeration of presumptive Escherichia coli in food and animal feed through the MPN method, widely used for assessing microbial contamination and food safety.Performance Testing of Culture Media and Reagents
A major update in this amendment is the inclusion of performance testing protocols for culture media and reagents. This ensures that laboratories verify the productivity, selectivity, and detection capabilities of media and reagents before use, as described in ISO 11133.Updated References and Procedures
The amendment replaces earlier references (ISO 8261, ISO/TS 11133-1/-2) with the consolidated ISO 11133 standard, which governs preparation, storage, and performance testing of microbiological culture media and reagents.Quality Assurance Table for Media and Reagents
The standard provides detailed performance testing criteria, including incubation conditions, strain requirements, and measurable indicators such as gas production in Durham tubes, turbidity, and color formation. These ensure consistent microbial growth and detection reliability.Laboratory Practices
Laboratories are directed to follow general microbiological practices per ISO 7218 and ISO 11133, covering preparation, storage, and usage guidelines for media and reagents to maintain test integrity.
Applications
ISO 7251:2005/Amd 1:2023 is highly relevant across multiple sectors concerned with food safety, animal feed quality, and public health, including:
Food Testing Laboratories
Ensuring reliable detection of Escherichia coli contamination in food products to comply with regulatory standards and protect consumer health.Animal Feed Quality Control
Screening feed materials to prevent microbial contamination that could affect animal health and enter human food chains.Microbiological Research
Applying standardized MPN techniques for microbiological enumeration and validation of media and reagents in research settings.Regulatory Compliance
Assisting food safety authorities and certification bodies in the consistent application of microbiological testing methods aligned with international standards.Manufacturers of Culture Media and Reagents
Enabling producers to benchmark and demonstrate the performance of their products based on internationally accepted quality assurance criteria.
Related Standards
ISO 11133 – Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
Provides comprehensive guidelines on culture media preparation and performance testing crucial to ISO 7251’s amendments.ISO 7218 – Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
Governs basic laboratory practices harmonized with the detection and enumeration methods in ISO 7251.ISO/IEC Directives, Part 1 and Part 2 – Procedures and Editorial Rules
These directives provide the framework for developing and maintaining ISO standards such as ISO 7251.
By incorporating rigorous performance testing protocols, ISO 7251:2005/Amd 1:2023 ensures enhanced accuracy, reproducibility, and confidence in the microbiological detection of Escherichia coli across the food and feed industries. Implementing this standard supports global food safety initiatives and quality control measures critical for safeguarding public health.
ISO 7251:2005/Amd 1:2023 - Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique — Amendment 1: Inclusion of performance testing of culture media and reagents Released:12. 10. 2023
ISO 7251:2005/Amd 1:2023 - Microbiologie des aliments — Méthode horizontale pour la recherche et le dénombrement d'Escherichia coli présumés — Technique du nombre le plus probable — Amendment 1: Inclusion des essais de performance des milieux de culture et réactifs Released:12. 10. 2023
Frequently Asked Questions
ISO 7251:2005/Amd 1:2023 is a standard published by the International Organization for Standardization (ISO). Its full title is "Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of presumptive Escherichia coli - Most probable number technique - Amendment 1: Inclusion of performance testing of culture media and reagents". This standard covers: Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of presumptive Escherichia coli - Most probable number technique - Amendment 1: Inclusion of performance testing of culture media and reagents
Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of presumptive Escherichia coli - Most probable number technique - Amendment 1: Inclusion of performance testing of culture media and reagents
ISO 7251:2005/Amd 1:2023 is classified under the following ICS (International Classification for Standards) categories: 07.100.30 - Food microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
ISO 7251:2005/Amd 1:2023 has the following relationships with other standards: It is inter standard links to ISO 7251:2005. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ISO 7251:2005/Amd 1:2023 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
INTERNATIONAL ISO
STANDARD 7251
Third edition
2005-02-01
AMENDMENT 1
2023-10
Microbiology of food and animal
feeding stuffs — Horizontal method
for the detection and enumeration of
presumptive Escherichia coli — Most
probable number technique
AMENDMENT 1: Inclusion of
performance testing of culture media and
reagents
Microbiologie des aliments — Méthode horizontale pour la recherche
et le dénombrement d'Escherichia coli présumés — Technique du
nombre le plus probable
AMENDEMENT 1: Inclusion des essais de performance des milieux de
culture et réactifs
Reference number
ISO 7251:2005/Amd.1:2023(E)
ISO 7251:2005/Amd.1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
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ISO 7251:2005/Amd.1:2023(E)
Foreword
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iii
ISO 7251:2005/Amd.1:2023(E)
Mic
...
NORME ISO
INTERNATIONALE 7251
Troisième édition
2005-02-01
AMENDEMENT 1
2023-10
Microbiologie des aliments —
Méthode horizontale pour la
recherche et le dénombrement
d'Escherichia coli présumés —
Technique du nombre le plus probable
AMENDEMENT 1: Inclusion des essais
de performance des milieux de culture et
réactifs
Microbiology of food and animal feeding stuffs — Horizontal method
for the detection and enumeration of presumptive Escherichia coli —
Most probable number technique
AMENDMENT 1: Inclusion of performance testing of culture media
and reagents
Numéro de référence
ISO 7251:2005/Amd.1:2023(F)
ISO 7251:2005/Amd.1:2023(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2023
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être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: +41 22 749 01 11
E-mail: copyright@iso.org
Web: www.iso.org
Publié en Suisse
ii
ISO 7251:2005/Amd.1:2023(F)
Avant-propos
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ISO 7251:2005/Amd.1:2023(F)
Microbiologie des al
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