EN ISO 18187:2018

SLOVENSKI STANDARD
01-julij-2018
Kakovost tal - Kontaktni preskus za trdne vzorce z dehidrogenazno aktivnostjo
Arthrobacter globiformis (ISO 18187:2016)
Soil quality - Contact test for solid samples using the dehydrogenase activity of
Arthrobacter globiformis (ISO 18187:2016)
Bodenbeschaffenheit - Feststoffkontakttest unter Verwendung der
Dehydrogenaseaktivität von Arthrobacter globiformis (ISO 18187:2016)
Qualité du sol - Essai contact pour échantillons solides utilisant l'activité déshydrogénase
de Arthrobacter globiformis (ISO 18187:2016)
Ta slovenski standard je istoveten z: EN ISO 18187:2018
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18187
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2018
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Contact test for solid samples using the
dehydrogenase activity of Arthrobacter globiformis (ISO
18187:2016)
Qualité du sol - Essai contact pour échantillons solides Bodenbeschaffenheit - Feststoffkontakttest unter
utilisant l'activité déshydrogénase de Arthrobacter Verwendung der Dehydrogenaseaktivität von
globiformis (ISO 18187:2016) Arthrobacter globiformis (ISO 18187:2016)
This European Standard was approved by CEN on 14 February 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18187:2018 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 18187:2016 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 18187:2018 by
Technical Committee CEN/TC 444 “Test methods for environmental characterization of solid matrices”
the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2018, and conflicting national standards shall
be withdrawn at the latest by August 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 18187:2016 has been approved by CEN as EN ISO 18187:2018 without any modification.

INTERNATIONAL ISO
STANDARD 18187
First edition
2016-05-01
Soil quality — Contact test for solid
samples using the dehydrogenase
activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides
utilisant l’activité déshydrogénase de Arthrobacter globiformis
Reference number
ISO 18187:2016(E)
©
ISO 2016
ISO 18187:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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ii © ISO 2016 – All rights reserved

ISO 18187:2016(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms . 4
5.2 Control substrates . 4
5.2.1 General. 4
5.2.2 Control for soils . 4
5.2.3 Control for waste material . 5
5.3 Test substrates . 5
5.4 Chemicals . 6
6 Apparatus . 8
7 Procedure. 9
7.1 Preparation of dilutions . 9
7.2 Reference substance and positive control preparation . 9
7.3 Contact test procedure . 9
7.3.1 General. 9
7.3.2 Aeration .10
7.3.3 Deactivation .10
7.3.4 Preparation of the inoculum .11
7.3.5 Incubation and fluorescence measurement .11
7.4 Interferences .11
8 Calculation and expression of results .12
8.1 Calculation .12
8.1.1 Relative fluorescence .12
8.1.2 Determining the percentage of inhibition .12
8.2 Expression of results .12
9 Validity of the test .13
10 Statistical analysis .13
11 Test report .14
Annex A (informative) Results on the ring test .15
Annex B (informative) Preparation of test organisms .21
Annex C (informative) Testing chemical substances .23
Bibliography .25
ISO 18187:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or a
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