EN ISO 54321:2021

SLOVENSKI STANDARD
01-april-2021
Tla, obdelani biološki odpadki, blato in odpadki - Razklop frakcij elementov, topnih
v zlatotopki (ISO 54321:2020)
Soil, treated biowaste, sludge and waste - Digestion of aqua regia soluble fractions of
elements (ISO 54321:2020)
Boden, behandelter Bioabfall, Schlamm und Abfall - Aufschluss von mit Königswasser
löslichen Anteilen von Elementen (ISO 54321:2020)
Sols, biodéchets traités, boues et déchets - Digestion des éléments solubles dans l’eau
régale (ISO 54321:2020)
Ta slovenski standard je istoveten z: EN ISO 54321:2021
ICS:
13.030.20 Tekoči odpadki. Blato Liquid wastes. Sludge
13.080.10 Kemijske značilnosti tal Chemical characteristics of
soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 54321
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2021
EUROPÄISCHE NORM
ICS 13.030.20; 13.080.10
English Version
Soil, treated biowaste, sludge and waste - Digestion of aqua
regia soluble fractions of elements (ISO 54321:2020)
Sols, biodéchets traités, boues et déchets - Digestion Boden, behandelter Bioabfall, Schlamm und Abfall -
des éléments solubles dans l'eau régale (ISO Aufschluss von mit Königswasser löslichen Anteilen
54321:2020) von Elementen (ISO 54321:2020)
This European Standard was approved by CEN on 18 July 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 54321:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 54321:2021) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2021, and conflicting national standards shall be
withdrawn at the latest by July 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 54321:2020 has been approved by CEN as EN ISO 54321:2021 without any modification.

INTERNATIONAL ISO
STANDARD 54321
First edition
2020-08
Soil, treated biowaste, sludge and
waste — Digestion of aqua regia
soluble fractions of elements
Sols, biodéchets traités, boues et déchets — Digestion des éléments
solubles dans l’eau régale
Reference number
ISO 54321:2020(E)
©
ISO 2020
ISO 54321:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
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Email: copyright@iso.org
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Published in Switzerland
ii © ISO 2020 – All rights reserved

ISO 54321:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Interferences and sources of errors . 2
6 Reagents . 3
7 Apparatus . 3
7.1 General . 3
7.2 Method A — Apparatus for thermal heating under atmospheric conditions . 3
7.2.1 Method A1 — Thermal heating under reflux conditions . 3
7.2.2 Method A2 — Thermal heating with a heating block with containers . 4
7.3 Method B — Microwave digestion with temperature control, closed vessels . 4
8 Procedure. 5
8.1 General . 5
8.2 Blank test . 5
8.3 Method A — thermal heating under atmospheric conditions . 5
8.3.1 Method A1 — Thermal heating under reflux conditions . 5
8.3.2 Method A2 — Thermal heating with a heating block with containers . 6
8.4 Method B — Microwave digestion with temperature control, closed vessels . 7
9 Test report . 7
Annex A (informative) Repeatability and reproducibility data for soil, biowaste and sludge
samples . 9
Annex B (informative) Repeatability and reproducibility data for waste samples .10
Bibliography .37
ISO 54321:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee
SC 3, Chemical and physical characterization, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 444, Environmental characterization of solid
matrices, in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna
Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

ISO 54321:2020(E)
Introduction
Regarding the comparability of the procedure described in this document with those of the other
standards mentioned above the next remarks can be made:
— This document describes the digestion of solid samples with aqua regia.
— Differences in the procedures of the different standards are small. An important difference between
the reflux procedures as described in ISO 11466 and EN 13657 and EN 16174 concerns the waiting
time after addition of the acid to the sample, before the digestion starts. ISO 11466 specifies a
waiting time of 16 h, both European standards state that the digestion can start after the first strong
reactions have ceased. In validation work it was proven that the difference between 2 h and 16 h of
waiting was negligible, therefore this document follows the approach of EN 13657 and EN 16174.
— The heating block procedure was added to the reflux and microwave digestion procedures. The
procedure was adopted from the Dutch standard NEN 6961, which specifies a boiling time of 2 h to
4 h. This document specifies a boiling time of 2 h.
The methods specified in this document are providing multi-element aqua regia digestion techniques
for soil, treated biowaste, sludge and waste prior to analysis. It is known that the digestion of
environmental samples with aqua regia will not necessarily lead to complete element recoveries, and
that the extract from a test sample ma
...