EN ISO 14189:2016

SLOVENSKI STANDARD
01-junij-2017
Kakovost vode - Ugotavljanje števila Clostridium perfringens - Metoda
membranske filtracije (ISO 14189:2013)
Water quality - Enumeration of Clostridium perfringens - Method using membrane
filtration (ISO 14189:2013)
Wasserbeschaffenheit - Zählung von Clostridium perfringens - Verfahren mittels
Membranfiltration (ISO 14189:2013)
Qualité de l'eau - Dénombrement de Clostridium perfringens - Méthode de filtration sur
membrane (ISO 14189:2013)
Ta slovenski standard je istoveten z: EN ISO 14189:2016
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 14189
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2016
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - Enumeration of Clostridium perfringens -
Method using membrane filtration (ISO 14189:2013)
Qualité de l'eau - Dénombrement de Clostridium Wasserbeschaffenheit - Zählung von Clostridium
perfringens - Méthode de filtration sur membrane (ISO perfringens - Verfahren mittels Membranfiltration (ISO
14189:2013) 14189:2013)
This European Standard was approved by CEN on 15 July 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14189:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
The text of ISO 14189:2013 has been prepared by Technical Committee ISO/TC 147 “Water quality” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 14189:2016
by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2017, and conflicting national standards
shall be withdrawn at the latest by February 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 14189:2013 has been approved by CEN as EN ISO 14189:2016 without any modification.

INTERNATIONAL ISO
STANDARD 14189
First edition
2013-11-01
Water quality — Enumeration of
Clostridium perfringens — Method
using membrane filtration
Qualité de l’eau — Dénombrement de Clostridium perfringens —
Méthode de filtration sur membrane
Reference number
ISO 14189:2013(E)
©
ISO 2013
ISO 14189:2013(E)
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2013 – All rights reserved

ISO 14189:2013(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Apparatus and glassware . 2
6 Culture media and reagents . 3
6.1 Basic materials . 3
6.2 Culture media . 3
7 Sampling . 3
8 Procedure. 3
8.1 Transport and storage of the sample . 3
8.2 Heat pre-treatment to select spores . 3
8.3 Sample dilution . 3
8.4 Filtration . 4
8.5 Incubation and examination . 4
8.6 Confirmation of Clostridium perfringens . 4
9 Expression of results . 5
10 Test report . 5
11 Quality assurance . 5
Annex A (normative) Composition and preparation of culture media and reagents .7
Annex B (informative) Performance data . 9
Bibliography .12
ISO 14189:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
iv © ISO 2013 – All rights reserved

ISO 14189:2013(E)
Introduction
Clostridium perfringens is widely recognized as a valuable indicator for faecal pollution. Within the
intestinal tract of animals and man, these Gram-positive bacteria form spores which are resistant to
heating compared with vegetative cells. C. perfringens in the intestine exists both as spores and vegetative
cells, spores are also found in environmental samples. The spores of C. perfringens survive in water for
months, much longer than vegetative faecal indicator bacteria and consequently their presence may
indicate remote or intermittent faecal pollution. Monitoring of C. perfringens has proven useful for the
assessment of the quality of water resources and to check the stages of water treatment to evaluate the
treatment-works performance. The spores are not always inactivated by routine disinfection procedures
(e.g. chlorination).
INTERNATIONAL STANDARD ISO 14189:2013(E)
Water quality — Enumeration of Clostridium perfringens —
Method using membrane filtration
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document
be carried out by suitably qualified staff.
1 Scope
This International Standard specifies a method for the enumeration of vegetative ce
...