EN ISO 11731:2017
(Main)Water quality - Enumeration of Legionella (ISO 11731:2017)
Water quality - Enumeration of Legionella (ISO 11731:2017)
ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.
These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.
Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.
Wasserbeschaffenheit - Zählung von Legionellen (ISO 11731:2017)
Dieses Dokument legt Kultivierungsverfahren zur Isolierung von Legionella und Abschätzung ihrer Anzahl in Wasserproben fest.
Diese Verfahren sind für alle Arten von Wasser, einschließlich Trinkwasser, Brauchwasser, Abwasser und natürliche Gewässer, anwendbar. Diese Verfahren können für wasserhaltige Matrices, z. B. Biofilme, Sedimente usw., verwendet werden.
Nicht alle Legionella-Spezies sind kultivierbar; deshalb werden mit den in dem vorliegenden Dokument beschriebenen Verfahren nicht alle Spezies von Legionella gefunden.
Qualité de l'eau - Dénombrement des Legionella (ISO 11731:2017)
ISO 11731:2017 spécifie des méthodes de culture pour l'isolement des Legionella et leur dénombrement dans des échantillons d'eau.
Ces méthodes sont applicables à tous les types d'échantillons d'eau, y compris les eaux potables, industrielles, usées et naturelles. Elles peuvent également être utilisées pour les matrices liées à l'eau (biofilms, sédiments, etc.).
Toutes les espèces de Legionella n'étant pas cultivables, les méthodes décrites dans le présent document peuvent ne pas retrouver la totalité des espèces de Legionella.
Kakovost vode - Ugotavljanje števila legionel (ISO 11731:2017)
Ta dokument določa metode gojenja za izolacijo legionele in oceno njenega števila v vzorcih vode.
Te metode se uporabljajo za vse vrste vzorcev vode, vključno s pitnimi, industrijskimi, odpadnimi in
naravnimi vodami. Te metode se lahko uporabljajo za matrice, povezane z vodo, npr. biofilme, sedimente itd.
Nekaterih vrst legionele ni mogoče gojiti, tako da metode, opisane v tem dokumentu, ne
zajamejo vseh vrst legionele.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2017
1DGRPHãþD
SIST EN ISO 11731-2:2008
Kakovost vode - Ugotavljanje števila legionel (ISO 11731:2017)
Water quality - Enumeration of Legionella (ISO 11731:2017)
Wasserbeschaffenheit - Zählung von Legionellen (ISO 11731:2017)
Qualité de l'eau - Dénombrement des Legionella (ISO 11731:2017)
Ta slovenski standard je istoveten z: EN ISO 11731:2017
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 11731
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2017
EUROPÄISCHE NORM
ICS 07.100.20 Supersedes EN ISO 11731-2:2008
English Version
Water quality - Enumeration of Legionella (ISO
11731:2017)
Qualité de l'eau - Dénombrement des Legionella (ISO Wasserbeschaffenheit - Zählung von Legionellen (ISO
11731:2017) 11731:2017)
This European Standard was approved by CEN on 12 February 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11731:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 11731:2017) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2017, and conflicting national standards
shall be withdrawn at the latest by December 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 11731-2:2008.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11731:2017 has been approved by CEN as EN ISO 11731:2017 without any modification.
INTERNATIONAL ISO
STANDARD 11731
Second edition
2017-05
Water quality — Enumeration of
Legionella
Qualité de l’eau — Dénombrement des Legionella
Reference number
ISO 11731:2017(E)
©
ISO 2017
ISO 11731:2017(E)
© ISO 2017, Published in Switzerland
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ISO 11731:2017(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Examination. 2
4.3 Confirmation . 2
5 Apparatus and glassware . 2
6 Culture media and reagents . 3
7 Sampling . 4
8 Procedure. 4
8.1 Samples . 4
8.2 Concentration of water samples . 5
8.2.1 General. 5
8.2.2 Membrane filtration and direct placing of the membrane filter on culture media 5
8.2.3 Membrane filtration followed by a washing procedure . 5
8.3 Sample pre-treatment . 6
8.3.1 Heat treatment. 6
8.3.2 Acid treatment . 6
8.4 Culture . 6
8.4.1 General. 6
8.4.2 Samples with a high concentration of Legionella species and a low
concentration of interfering microorganisms . 6
8.4.3 Samples with a low concentration of Legionella species and a low
concentration of interfering microorganisms . 6
8.4.4 Samples with a high concentration of interfering microorganisms . 7
8.4.5 Samples with an extremely high concentration of interfering microorganisms . 7
8.4.6 Incubation . 7
8.4.7 Examination of the plates . 7
8.5 Confirmation of presumptive Legionella colonies on culture media: BCYE agar and
BCYE–cys agar . 8
9 Expression of results . 8
10 Test report . 9
11 Quality assurance .10
11.1 General .10
11.2 Performance testing of Legionella culture media .10
11.3 Preparing working culture and test suspension for performance testing .10
Annex A (informative) Legionella species .12
Annex B (normative) Culture media .14
Annex C (normative) Diluents .20
Annex D (normative) Acid solution.21
Annex E (informative) Scraping or rubbing the bacteria from membrane filters .22
Annex F (informative) Centrifugation technique .23
ISO 11731:2017(E)
Annex G (informative) Indirect immunofluorescent antibody assay for the identification of
Legionella species .24
Annex H (informative) Performance data .27
Annex I (informative) Pre-treatment of water related matrices .31
Annex J (normative) Decision matrix .32
Bibliography .
...
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