Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 3: Method using freeze-dried bacteria - Amendment 1 (ISO 11348-3:2007/Amd 1:2018)

Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 3: Verfahren mit gefriergetrockneten Bakterien - Änderung 1 (ISO 11348-3:2007/Amd 1:2018)

No scope available

Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 3: Méthode utilisant des bactéries lyophilisées - Amendement 1 (ISO 11348-3:2007/Amd 1:2018)

Kakovost vode - Določevanje zaviralnega učinka vzorcev vode na oddajanje svetlobe Vibrio fischeri (preskus luminiscence bakterije) - 3. del: Metoda z uporabo liofilizirane bakterije - Dopolnilo A1 (ISO 11348-3:2007/Amd 1:2018)

General Information

Status
Published
Publication Date
18-Dec-2018
Withdrawal Date
29-Jun-2019
Technical Committee
CEN/TC 230 - Water analysis
Drafting Committee
CEN/TC 230 - Water analysis
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
19-Dec-2018
Completion Date
19-Dec-2018

Relations

Effective Date
01-Mar-2017

Overview

EN ISO 11348-3:2008/A1:2018 amends the international method for assessing the inhibitory effect of water samples on the light emission of Vibrio fischeri - the luminescent bacteria test - specifically the variant that uses freeze-dried bacteria. Endorsed by CEN and prepared by ISO/TC 147 (Water quality), this amendment updates quality criteria within the existing Part 3 procedure (ISO 11348-3:2007) and is published in the three official CEN languages (EN/FR/DE).

Key topics and requirements

  • Test principle: measurement of reduced bioluminescence of Vibrio fischeri as an indicator of sample toxicity (freeze-dried bacterial reagent format).
  • Amendment focus: clarification of a precision/acceptance criterion in Clause 11 (third bullet) related to dilutions used to determine the LID‑value - the amendment specifies that, for the test sample dilutions determining the LID-value, the deviation from their mean (expressed in “percent points”) must not exceed 3 percentage points (see footnote c in Table 1).
  • Quality control: tighter reproducibility/data acceptance rules for sample dilution results and reporting to ensure reliable interpretation of inhibitory effects.
  • Standards governance: harmonized adoption across CEN member bodies; users should check national implementation dates and any national forewords.

Applications

  • Environmental laboratories performing water quality testing and toxicity screening of surface water, effluents and treated wastewater.
  • Regulatory agencies and compliance programs requiring standardized ecotoxicological assays.
  • Contract testing and research facilities using luminescent bacteria tests for rapid, cost‑effective screening of chemical and biological contaminants.
  • Manufacturers and suppliers of freeze‑dried Vibrio fischeri test kits who need to align product instructions and QC limits with the amended standard.
  • Quality managers and method developers applying standard data acceptance criteria and reproducibility thresholds in routine monitoring.

Who should use this standard

  • Environmental and public-health laboratories
  • Water utilities and wastewater treatment operators
  • Regulatory bodies and accreditation assessors
  • Ecotoxicologists and environmental consultants
  • Test kit producers and laboratory equipment vendors

Related standards

  • This document is part of the ISO 11348 series on luminescent bacteria testing. For details on other parts and methods (fresh cultures, alternative procedures), consult the ISO website or national standards body.

Keywords: EN ISO 11348-3, ISO 11348-3:2007/Amd 1:2018, luminescent bacteria test, Vibrio fischeri, freeze-dried bacteria method, water quality testing, LID-value, ecotoxicology, CEN.

Amendment

EN ISO 11348-3:2009/A1:2019

English language
7 pages
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Frequently Asked Questions

EN ISO 11348-3:2008/A1:2018 is a amendment published by the European Committee for Standardization (CEN). Its full title is "Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 3: Method using freeze-dried bacteria - Amendment 1 (ISO 11348-3:2007/Amd 1:2018)". This standard covers: No scope available

No scope available

EN ISO 11348-3:2008/A1:2018 is classified under the following ICS (International Classification for Standards) categories: 07.100.20 - Microbiology of water; 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.

EN ISO 11348-3:2008/A1:2018 has the following relationships with other standards: It is inter standard links to EN ISO 11348-3:2008. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.

You can purchase EN ISO 11348-3:2008/A1:2018 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.

Standards Content (Sample)


SLOVENSKI STANDARD
01-maj-2019
.DNRYRVWYRGH'RORþHYDQMH]DYLUDOQHJDXþLQNDY]RUFHYYRGHQDRGGDMDQMH
VYHWOREH9LEULRILVFKHUL SUHVNXVOXPLQLVFHQFHEDNWHULMH GHO0HWRGD]XSRUDER
OLRILOL]LUDQHEDNWHULMH'RSROQLOR$ ,62$PG
Water quality - Determination of the inhibitory effect of water samples on the light
emission of Vibrio fischeri (Luminescent bacteria test) - Part 3: Method using freeze-dried
bacteria - Amendment 1 (ISO 11348-3:2007/Amd 1:2018)
Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die
Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 3: Verfahren mit
gefriergetrockneten Bakterien - Änderung 1 (ISO 11348-3:2007/Amd 1:2018)
Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la
luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 3: Méthode
utilisant des bactéries lyophilisées - Amendement 1 (ISO 11348-3:2007/Amd 1:2018)
Ta slovenski standard je istoveten z: EN ISO 11348-3:2008/A1:2018
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11348-3:2008/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2018
EUROPÄISCHE NORM
ICS 13.060.70
English Version
Water quality - Determination of the inhibitory effect of
water samples on the light emission of Vibrio fischeri
(Luminescent bacteria test) - Part 3: Method using freeze-
dried bacteria - Amendment 1 (ISO 11348-3:2007/Amd
1:2018)
Qualité de l'eau - Détermination de l'effet inhibiteur Wasserbeschaffenheit - Bestimmung der
d'échantillons d'eau sur la luminescence de Vibrio Hemmwirkung von Wasserproben auf die
fischeri (Essai de bactéries luminescentes) - Partie 3: Lichtemission von Vibrio fischeri
Méthode utilisant des bactéries lyophilisées - (Leuchtbakterientest) - Teil 3: Verfahren mit
Amendement 1 (ISO 11348-3:2007/Amd 1:2018) gefriergetrockneten Bakterien - Änderung 1 (ISO
11348-3:2007/Amd 1:2018)
This amendment A1 modifies the European Standard EN ISO 11348-3:2008; it was approved by CEN on 18 December 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11348-3:2008/A1:2018 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11348-3:2008/A1:2018) has been prepared by Technical Committee ISO/TC
147 "Water quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2019, and conflicting national standards shall be
withdrawn at the latest by June 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croati
...

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