EN ISO 17512-1:2020
(Main)Soil quality - Avoidance test for determining the quality of soils and effects of chemicals on behaviour - Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei) (ISO 17512-1:2008)
Soil quality - Avoidance test for determining the quality of soils and effects of chemicals on behaviour - Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei) (ISO 17512-1:2008)
ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour.
The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test.
Two different designs (a two section unit and a six section unit) have been developed and successfully applied. Both designs are applicable to either single-concentration (e. g. for assessing the quality of a field soil) or multi-concentration (e. g. for assessing the toxicity of a spiked chemical) tests. In both cases, the earthworms are allowed to make the initial choice on which compartment, control and a treatment [in the two section test vessel between right and left side; in the six section test vessel between the (3 + 3) alternating compartments], to enter.
Bodenbeschaffenheit - Vermeidungsprüfung zur Bestimmung der Bodenbeschaffenheit und der Auswirkungen von Chemikalien auf das Verhalten - Teil 1: Prüfung von Regenwürmern (Eisenia fetida und Eisenia andrei) (ISO 17512-1:2008)
Dieser Teil von ISO 17512 legt ein Screening-Schnellverfahren zur Bewertung der Lebensraumfunktion von Böden und des Einflusses von Verunreinigungen und Chemikalien auf das Verhalten von Regenwürmern fest.
Die subletale Prüfung ist ein Schnellverfahren, das die Bioverfügbarkeit von Gemischen aus Verunreinigungen in natürlichen Böden und von in Böden eingemischten Substanzen für Eisenia fetida und Eisenia andrei wiedergibt. Das Vermeidungsverhalten der Würmer stellt den Endpunkt der Prüfung dar. Die Prüfung ist nicht als Ersatz für den Regenwurm Reproduktionstest vorgesehen.
Zwei unterschiedliche Versuchsanordnungen (ein Zweikammerprüfgefäß und ein Sechskammerprüfgefäß) wurden entwickelt und erfolgreich eingesetzt. Beide Versuchsanordnungen sind sowohl für Prüfungen auf Einzelkonzentrationen (z. B. zur Bewertung der Beschaffenheit eines Feldbodens) als auch für Prüfungen auf Mehrfachkonzentrationen (z. B. zur Bewertung der Toxizität einer zugesetzten Chemikalie) anwendbar. In beiden Fällen ist es den Regenwürmern überlassen anfänglich zu wählen, in welches Segment, ob in den Kontroll oder Prüfboden, sie eindringen [im Zweikammerprüfgefäß zwischen der linken und der rechten Seite; im Sechskammerprüfgefäß zwischen den (3 + 3) sich abwechselnden Kammern].
Qualité du sol - Essai d'évitement pour contrôler la qualité des sols et les effets des produits chimiques sur le comportement - Partie 1: Essai avec des vers de terre (Eisenia fetida et Eisenia andrei) (ISO 17512-1:2008)
L'ISO 17512-1:2008 spécifie une méthode d'essai biologique de dépistage rapide permettant d'évaluer la fonction d'habitat des sols ainsi que l'influence des contaminants et des produits chimiques sur le comportement des vers de terre.
Cet essai sublétal constitue une méthode rapide qui reflète la biodisponibilité pour les espèces Eisenia fetida et Eisenia andrei des mélanges de contaminants dans les sols naturels et des sols dopés en substances. Le comportement d'évitement des vers constitue le critère d'effet de cet essai. Ce dernier n'est pas destiné à remplacer l'essai de reproduction des vers de terre.
Deux modèles de conception différente (récipient d'essai à deux compartiments et récipient d'essai à six compartiments) ont été mis au point et mis en œuvre avec succès. Les deux modèles conviennent soit aux essais à une seule concentration (par exemple pour évaluer la qualité d'un sol naturel), soit aux essais à plusieurs concentrations (par exemple pour évaluer la toxicité d'un sol dopé par un produit chimique). Dans les deux cas, on laisse aux vers de terre le choix initial du compartiment où pénétrer: sol témoin et un traitement [dans le récipient d'essai à deux compartiments, choix entre le côté droit ou gauche; dans le récipient d'essai à six compartiments, choix entre les compartiments disposés de façon alternée (3 + 3)].
Kakovost tal - Izogibalni preskus za določanje kakovosti tal in učinkov kemikalij na obnašanje - 1. del: Preskus z deževniki (Eisenia fetida in Eisenia andrei) (ISO 17512-1:2008)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Izogibalni preskus za določanje kakovosti tal in učinkov kemikalij na
obnašanje - 1. del: Preskus z deževniki (Eisenia fetida in Eisenia andrei) (ISO
17512-1:2008)
Soil quality - Avoidance test for determining the quality of soils and effects of chemicals
on behaviour - Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei) (ISO
17512-1:2008)
Bodenbeschaffenheit - Vermeidungsprüfung zur Bestimmung der Bodenbeschaffenheit
und der Auswirkungen von Chemikalien auf das Verhalten - Teil 1: Prüfung von
Regenwürmern (Eisenia fetida und Eisenia andrei) (ISO 17512-1:2008)
Qualité du sol - Essai d'évitement pour contrôler la qualité des sols et les effets des
produits chimiques sur le comportement - Partie 1: Essai avec des vers de terre (Eisenia
fetida et Eisenia andrei) (ISO 17512-1:2008)
Ta slovenski standard je istoveten z: EN ISO 17512-1:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 17512-1
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Avoidance test for determining the quality of
soils and effects of chemicals on behaviour - Part 1: Test
with earthworms (Eisenia fetida and Eisenia andrei) (ISO
17512-1:2008)
Qualité du sol - Essai d'évitement pour contrôler la Bodenbeschaffenheit - Vermeidungsprüfung zur
qualité des sols et les effets des produits chimiques sur Bestimmung der Bodenbeschaffenheit und der
le comportement - Partie 1: Essai avec des vers de Auswirkungen von Chemikalien auf das Verhalten -
terre (Eisenia fetida et Eisenia andrei) (ISO 17512- Teil 1: Prüfung von Regenwürmern (Eisenia fetida und
1:2008) Eisenia andrei) (ISO 17512-1:2008)
This European Standard was approved by CEN on 13 April 2020.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
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© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17512-1:2020 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 17512-1:2008 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 17512-
1:2020 by Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the
secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 17512-1:2008 has been approved by CEN as EN ISO 17512-1:2020 without any
modification.
INTERNATIONAL ISO
STANDARD 17512-1
First edition
2008-12-15
Soil quality — Avoidance test for
determining the quality of soils and
effects of chemicals on behaviour —
Part 1:
Test with earthworms (Eisenia fetida and
Eisenia andrei)
Qualité du sol — Essai d'évitement pour contrôler la qualité des sols et
les effets des produits chimiques sur le comportement —
Partie 1: Essai avec des vers de terre (Eisenia fetida et Eisenia andrei)
Reference number
ISO 17512-1:2008(E)
©
ISO 2008
ISO 17512-1:2008(E)
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ii © ISO 2008 – All rights reserved
ISO 17512-1:2008(E)
Contents Page
Foreword .iv
Introduction.v
1 Scope.1
2 Normative references.1
3 Terms and definitions .2
4 Principle .2
5 Reagents and materials .3
6 Apparatus.4
7 Procedure.5
7.1 Appropriate concentration range .5
7.2 Testing of soils .5
7.3 Testing of chemical.6
7.4 Reference substance .6
7.5 Validity criteria.6
8 Calculation and expression of results .6
9 Test report.7
Annex A (informative) Test chambers .8
Annex B (informative) Example of a breeding technique for Eisenia fetida and Eisenia andrei.11
Annex C (informative) Further test organisms.12
Annex D (informative) Contaminants that earthworms can detect and avoid in the avoidance test.13
Annex E (normative) Testing of chemicals in the avoidance test .14
Annex F (normative) Determination of water-holding capacity .16
Annex G (informative) Comparison of the results obtained in the two section chamber and six
section chamber system .17
Annex H (informative) Influence of soil properties on avoidance behaviour — Basis for the
threshold value of 20 % .20
Annex I (informative) Data gained in “dual” tests with the same (untreated) control soil on both
sides of the test vessels .22
Bibliography.24
ISO 17512-1:2008(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17512-1 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
ISO 17512 consists of the following parts, under the general title Soil quality — Avoidance test for determining
the quality of soils and effects of chemicals on behaviour:
⎯ Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei)
The following part is under preparation:
⎯ Part 2: Test with collembolans (Folsomia candida)
iv © ISO 2008 – All rights reserved
ISO 17512-1:2008(E)
Introduction
Ecotoxicological test systems are applied to obtain information about the effects of contaminants in soil and
are proposed to complement conventional chemical analysis (see ISO 15799). ISO 15799 includes a list and
short characterisation of recommended and standardised test systems. Aquatic test systems with soil eluate
are applied to obtain information about the fraction of contaminants potentially reaching the groundwater by
the water path (retention function of soils), whereas terrestrial test systems are used to assess the habitat
function of soils. As standardised test systems, a mortality test (ISO 11268-1) and a reproduction test
(ISO 11268-2) exist to investigate the habitat function of a soil with respect to earthworms as representatives
of the soil biocenosis.
The reproduction test with earthworms (ISO 11268-2) is applied to detect effects resulting from sublethal
concentrations. Such endpoints are preferably applied to obtain information on environmental effects.
However, the reproduc
...
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