EN ISO 14238:2013

SLOVENSKI STANDARD
01-marec-2014
.DNRYRVWWDO%LRORãNHPHWRGH'RORþHYDQMHPLQHUDOL]DFLMHGXãLNDLQQLWULILNDFLMH
YWOHKWHUYSOLYNHPLNDOLMQDWHSURFHVH,62
Soil quality - Biological methods - Determination of nitrogen mineralization and
nitrification in soils and the influence of chemicals on these processes (ISO 14238:2012)
Bodenbeschaffenheit - Biologische Verfahren - Bestimmung der Stickstoffmineralisierung
und -nitrifizierung in Böden und der Einflüsse von Chemikalien auf diese Prozesse (ISO
14238:2012)
Qualité du sol - Méthodes biologiques - Détermination de la minéralisation de l'azote et
de la nitrification dans les sols, et de l'influence des produits chimiques sur ces
processus (ISO 14238:2012)
Ta slovenski standard je istoveten z: EN ISO 14238:2013
ICS:
13.080.10 .HPLMVNH]QDþLOQRVWLWDO Chemical characteristics of
soils
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 14238
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2013
ICS 13.080.30
English Version
Soil quality - Biological methods - Determination of nitrogen
mineralization and nitrification in soils and the influence of
chemicals on these processes (ISO 14238:2012)
Qualité du sol - Méthodes biologiques - Détermination de la Bodenbeschaffenheit - Biologische Verfahren - Bestimmung
minéralisation de l'azote et de la nitrification dans les sols, der Stickstoffmineralisierung und -nitrifizierung in Böden
et de l'influence des produits chimiques sur ces processus und der Einflüsse von Chemikalien auf diese Prozesse (ISO
(ISO 14238:2012) 14238:2012)
This European Standard was approved by CEN on 10 November 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14238:2013 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 14238:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organisation for Standardization (ISO) and has been taken over as EN ISO 14238:2013 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2014, and conflicting national standards shall be withdrawn at
the latest by June 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 14238:2012 has been approved by CEN as EN ISO 14238:2013 without any modification.

INTERNATIONAL ISO
STANDARD 14238
Second edition
2012-08-01
Soil quality — Biological methods —
Determination of nitrogen mineralization
and nitrification in soils and the influence
of chemicals on these processes
Qualité du sol — Méthodes biologiques — Détermination de la
minéralisation de l’azote et de la nitrification dans les sols, et de
l’influence des produits chimiques sur ces processus
Reference number
ISO 14238:2012(E)
©
ISO 2012
ISO 14238:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2012 – All rights reserved

ISO 14238:2012(E)
Contents Page
Foreword .iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Materials . 2
5.1 Soils . 2
5.2 Reagents and materials . 3
5.3 Test substance . 3
6 Apparatus . 4
7 Procedures . 4
7.1 Experimental options . 4
7.2 Treatment of soils . 4
7.3 Incubation of soils . 5
7.4 Sampling of soils for testing . 6
7.5 Extraction of soils . 6
7.6 Analyses . 6
8 Expression of results . 6
8.1 Basic mineralization test . 6
8.2 Toxicity testing . 7
9 Test report . 8
Annex A (informative) Determination of water-holding capacity of soil .10
Bibliography .12
ISO 14238:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 14238 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods.
This second edition cancels and replaces the first edition (ISO 14238:1997), which has been technically revised.
iv © ISO 2012 – All rights reserved

ISO 14238:2012(E)
Introduction
Soil consists of both living and non-living components which exist in a complex and heterogeneous environment.
Microorganisms in the soil are mainly responsible for cycling of some nutrients and thus play an essential role
in the maintenance of soil fertility. One of the most important microbial processes in soil is the mineralization
of nitrogen contained in organic forms to ammonium (ammonification) and thereafter to nitrite and nitrate
(nitrification). Clearly, any long-term interference with this process could influence soil fertility.
INTERNATIONAL STANDARD ISO 14238:2012(E)
Soil quality — Biological methods — Determination of nitrogen
mineralization and nitrification in soils and the influence of
chemicals on these processes
1 Scope
This International Standard specifies laboratory procedures for measuring the mineralization and nitrification
of nitrogen by the soil microbiota.
For investigations on the evaluation of soil quality or on effects of contamination, a procedure is given to
measure the rates and extent of N-mineralization in soil or soils of known or unknown quality.
For investigation of the potential toxicity of chemicals to N-mineralization in soils, a simple procedure is given
which allows the impact of single chemicals to be assessed and provides a basis for comparison of the toxicities
of different chemicals.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced document
(including any amendments) applies.
ISO 10381-6, Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil under
aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390, Soil quality — Determination of pH
ISO 10694, Soil quality — Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260, Soil quality — Determination of effective cation exchange capacity and base saturation level using
barium chloride solution
ISO 11261, Soil quality — Determination of total nitrogen — Modified Kjedahl method
ISO 11274, Soil quality — Determination of the water-
...