EN ISO 16373-2:2014

SLOVENSKI STANDARD
01-september-2014
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Textiles - Dyestuffs - Part 2: General method for the determination of extractable
dyestuffs including allergenic and carcinogenic substances (ISO 16373-2:2014)
Textilien - Farbstoffe - Teil 2: Allgemeines Verfahren zur Bestimmung von bestimmten
extrahierbaren Farbstoffen einschließlich allergener und karzinogener Farbstoffe (ISO
16373-2:2014)
Textiles - Colorants - Partie 2: Méthode générale pour la détermination de colorants
extractibles dont les substances allergènes et carcinogènes (ISO 16373-2:2014)
Ta slovenski standard je istoveten z: EN ISO 16373-2:2014
ICS:
59.080.01 Tekstilije na splošno Textiles in general
87.060.10 Pigmenti in polnila Pigments and extenders
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16373-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2014
ICS 59.080.01
English Version
Textiles - Dyestuffs - Part 2: General method for the
determination of extractable dyestuffs including allergenic and
carcinogenic dyestuffs (method using pyridine-water) (ISO
16373-2:2014)
Textiles - Colorants - Partie 2: Méthode générale de Textilien - Farbstoffe - Teil 2: Allgemeines Verfahren zur
détermination des colorants extractibles, notamment les Bestimmung von extrahierbaren Farbstoffen einschließlich
colorants allergènes et cancérigènes (méthode utilisant un allergener und karzinogener Farbstoffe (Pyridin-Wasser-
mélange pyridine/eau) (ISO 16373-2:2014) Verfahren) (ISO 16373-2:2014)
This European Standard was approved by CEN on 3 June 2014.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16373-2:2014 E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (EN ISO 16373-2:2014) has been prepared by Technical Committee CEN/TC 248 “Textiles
and textile products” the secretariat of which is held by BSI, in collaboration with Technical Committee
ISO/TC 38 "Textiles".
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by December 2014, and conflicting national standards shall be withdrawn
at the latest by December 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 16373-2:2014 has been approved by CEN as EN ISO 16373-2:2014 without any modification.

INTERNATIONAL ISO
STANDARD 16373-2
First edition
2014-06-01
Textiles — Dyestuffs —
Part 2:
General method for the determination
of extractable dyestuffs including
allergenic and carcinogenic dyestuffs
(method using pyridine-water)
Textiles — Colorants —
Partie 2: Méthode générale de détermination des colorants
extractibles, notamment les colorants allergènes et cancérigènes
(méthode utilisant un mélange pyridine/eau)
Reference number
ISO 16373-2:2014(E)
©
ISO 2014
ISO 16373-2:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 16373-2:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Safety precautions . 1
6 Reagents . 2
7 Apparatus . 2
7.1 Apparatus and auxiliaries for sample preparation . 2
7.2 Chromatographic equipment (selected from the following). 2
8 Procedure. 3
8.1 Preparation of test specimen . 3
8.2 Extraction . 3
8.3 Detection and quantification of dyestuff . 3
8.4 Calibration . 3
9 Calculation and expression of the results . 4
10 Test report . 4
Annex A (normative) List of carcinogenic dyestuffs . 5
Annex B (normative) List of allergenic and other dyestuffs . 6
Annex C (normative) Calculation . 7
Annex D (informative) Examples of chromatographic methods. 8
Annex E (informative) Reliability of the method .17
Annex F (informative) Multiple-extraction of several textile fibres with pyridine/water .18
Bibliography .20
ISO 16373-2:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received. www.iso.org/patents
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information
ISO 16373-2 was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 248, Textiles and textile products, in collaboration with ISO Technical Committee TC 38, Textiles,
in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
ISO 16373 consists of the following parts, under the general title Textiles — Dyestuffs:
— Part 1: General principles of testing coloured textiles for dyestuff identification
— Part 2: General method for the determination of extractable dyestuffs including allergenic and
carcinogenic (method using pyridine-water)
— Part 3: Method for determination of carcinogenic extractable dyestuffs (method using
triethylamine/methanol)
iv © ISO 2014 – All rights reserved

ISO 16373-2:2014(E)
Introduction
The ISO 16373 series deals with dyestuffs used in textile for qualification and quantification.
1)
ISO 16373-1 includes the definition of the dyestuff classes and the description of some procedures to
identify qualitatively the dyestuff class used in textile material.
The other parts of ISO 16373 are related to the quantification of some dyestuffs.
In this part of ISO 16373, the principle of the test method is based on the extraction using pyridine-water
...