EN ISO 23753-1:2019

SLOVENSKI STANDARD
01-julij-2019
Nadomešča:
SIST EN ISO 23753-1:2011
Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 1. del: Metoda s
trifeniltetrazolijevim kloridom (TTC) (ISO 23753-1:2019)
Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using
triphenyltetrazolium chloride (TTC) (ISO 23753-1:2019)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 1:
Verfahren mit Triphenyltetrazoliumchlorid (TTC) (ISO 23753-1:2019)
Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 1:
Méthode au chlorure de triphényltétrazolium (CTT) (ISO 23753-1:2019)
Ta slovenski standard je istoveten z: EN ISO 23753-1:2019
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23753-1
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2019
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 23753-1:2011
English Version
Soil quality - Determination of dehydrogenases activity in
soils - Part 1: Method using triphenyltetrazolium chloride
(TTC) (ISO 23753-1:2019)
Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der
déshydrogénases dans les sols - Partie 1: Méthode au Dehydrogenaseaktivität in Böden - Teil 1: Verfahren
chlorure de triphényltétrazolium (CTT) (ISO 23753- mit Triphenyltetrazoliumchlorid (TTC) (ISO 23753-
1:2019) 1:2019)
This European Standard was approved by CEN on 4 February 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-1:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23753-1:2019) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2019, and conflicting national standards
shall be withdrawn at the latest by September 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23753-1:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 23753-1:2019 has been approved by CEN as EN ISO 23753-1:2019 without any
modification.
INTERNATIONAL ISO
STANDARD 23753-1
Second edition
2019-02
Soil quality — Determination of
dehydrogenases activity in soils —
Part 1:
Method using triphenyltetrazolium
chloride (TTC)
Qualité du sol — Détermination de l'activité des déshydrogénases
dans les sols —
Partie 1: Méthode au chlorure de triphényltétrazolium (CTT)
Reference number
ISO 23753-1:2019(E)
©
ISO 2019
ISO 23753-1:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 23753-1:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Limitations . 1
6 Reagents and materials . 2
7 Apparatus . 3
8 Procedure. 3
8.1 Establishment of standard curve . 3
8.2 Sampling . 3
9 Calculation . 4
10 Validity criteria . 4
10.1 Standard curve . 4
10.2 Samples . 5
11 Test report . 5
Annex A (informative) Results of modified parameters . 6
Bibliography . 9
ISO 23753-1:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological characterization.
This second edition cancels and replaces the first edition (ISO 23753-1:2005), which has been
technically revised. The main changes compared to the previous edition are as follows:
— a new Clause 5 “Limitations” has been added;
— in Clause 6, reagents and their preparation have been updated to new results [e.g. use of less toxic
solvent (ethanol), substrate concentration of 60 mmol/l of TTC, concentration of Tris buffer of
100 mmol/l at pH 7,6, incubation time of 6 h];
— Tables 1 and 2 have been added;
— Clause 10 "Validity criteria" has been added;
— a new Annex A “Results of modified parameters” has been added;
— Clause 2 “Normative references” and the Bibliography have been updated.
A list of all the parts in the ISO 23753 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved

ISO 23753-1:2019(E)
Introduction
The soil microflora is responsible for the decomposition and conversion of organic substances, carbon,
nitrogen, sulfur and phosphorus cycles, soil aggregates stability and as a food source for microbivores.
Dehydrogenases, as intracellular enzymes and respiratory chain components of the microbial cells, play
a major role in the production of energy by organisms. They oxidize organic compounds by transferring
+
electrons to an acceptor (e.g. NAD ). Dehydrogenases are essential components of the enzyme system
of microorganisms. Dehydrogenases activity can therefore be used as an indicator of biological redox
systems and as a measure of the viable and physiologically active soil microbial community.
Microbial oxidative activity in soil is linked to respiratory activity, which could be approached with the
determination of dehydrogenases activity. Basal and induced respiration in soil could be affected by
soil management, practices and contamination.
...