EN ISO 16050:2011

SLOVENSKI STANDARD
01-oktober-2011
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SIST EN 12955:2000
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Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1
and G2 in cereals, nuts and derived products - High-performance liquid chromatographic
method (ISO 16050:2003)
Lebensmittel - Bestimmung von Aflatoxin B1 und der Summe von Aflatoxin B1, B2, G1
und G2 in Getreiden, Nüssen und verwandten Produkten -
Hochleistungsflüssigchromatographisches Verfahren (ISO 16050:2003)
Produits alimentaires - Dosage de l'aflatoxine B1 et détermination de la teneur totale en
aflatoxines B1, B2, G1 et G2 dans les céréales, les fruits à coque et les produits dérivés
- Méthode par chromatographie liquide à haute performance (ISO 16050:2003)
Ta slovenski standard je istoveten z: EN ISO 16050:2011
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
67.080.10 Sadje in sadni proizvodi Fruits and derived products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16050
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2011
ICS 67.060; 67.080.10 Supersedes EN 12955:1999
English Version
Foodstuffs - Determination of aflatoxin B1, and the total content
of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived
products - High-performance liquid chromatographic method
(ISO 16050:2003)
Produits alimentaires - Dosage de l'aflatoxine B1 et Lebensmittel - Bestimmung von Aflatoxin B1 und der
détermination de la teneur totale en aflatoxines B1, B2, G1 Summe von Aflatoxin B1, B2, G1 und G2 in Getreiden,
et G2 dans les céréales, les fruits à coque et les produits Nüssen und verwandten Produkten -
dérivés - Méthode par chromatographie liquide à haute Hochleistungsflüssigchromatographisches Verfahren (ISO
performance (ISO 16050:2003) 16050:2003)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16050:2011: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 16050:2003 has been prepared by Technical Committee ISO/TC 34 “Food products” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 16050:2011 by
Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 12955:1999.
Originally, EN 12955:1999 "Foodstuffs - Determination of aflatoxin B , and the sum of aflatoxins B B , G
1 1, 2 1
and G in cereals, shell-fruits and derived products - High performance liquid chromatographic method with
post column derivatization and immunoaffinity column clean up" was the basis for ISO 16050. In order to
avoid having two equal standards on CEN- and ISO-level on the same topic, it was decided to take over
ISO 16050 as EN ISO 16050 and to withdraw EN 12955 as soon as EN ISO 16050 is published.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 16050:2003 has been approved by CEN as a EN ISO 16050:2011 without any modification.

INTERNATIONAL ISO
STANDARD 16050
First edition
2003-09-01
Foodstuffs — Determination of aflatoxin
B , and the total content of aflatoxins B ,
1 1
B , G and G in cereals, nuts and
2 1 2
derived products — High-performance
liquid chromatographic method
Produits alimentaires — Dosage de l'aflatoxine B et détermination de
la teneur totale en aflatoxines B , B , G et G dans les céréales, les
1 2 1 2
fruits à coque et les produits dérivés — Méthode par chromatographie
liquide à haute performance
Reference number
ISO 16050:2003(E)
©
ISO 2003
ISO 16050:2003(E)
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ii © ISO 2003 — All rights reserved

ISO 16050:2003(E)
Contents Page
Foreword. iv
1 Scope. 1
2 Normative references. 1
3 Principle. 1
4 Reagents. 1
5 Apparatus. 4
6 Procedure. 5
6.1 General. 5
6.2 Extraction. 5
6.3 Clean-up. 6
6.4 HPLC operating conditions. 6
6.5 Identification. 6
6.6 Calibration graph. 6
6.7 Determination. 7
7 Calculation of results. 7
8 Precision. 8
8.1 Interlaboratory test. 8
8.2 Repeatability. 8
8.3 Reproducibility. 9
9 Test report. 9
Annex A (informative) Results of interlaboratory test . 10
Bibliography . 12

ISO 16050:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 16050 was prepared by Technical Committee ISO/TC 34, Food products. It is based on EN 12955:1999
elaborated by CEN/TC 275, Food analysis — Horizontal methods.

iv © ISO 2003 — All rights reserved

INTERNATIONAL STANDARD ISO 16050:2003(E)

Foodstuffs — Determination of aflatoxin B , and the total
content of aflatoxins B , B , G and G in cereals, nuts and
1 2 1 2
derived products — High-performance liquid chromatographic
method
WARNING — The use of this standard involves hazardous materials and operations. This standard
does not purport to address all the safety problems associated with its use. It is the responsibility of
the user of this standard to establish appropriate safety and health practice and to determine the
applicability of regulatory limitations prior to use.
1 Scope
This International Standard specifies a reverse-phase high-performance liquid chromatographic method, with
immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals,
nuts and derived products. The limit of quantification for aflatoxin B , and for the sum of aflatoxins B , B , G
1 1 2 1
and G , is 8 µg/kg.
The method has been validated for maize containing 24,5 µg/kg, for peanut butter containing 8,4 µg/kg, and
for raw peanuts containing 16 µg/kg of total aflatoxins. It has also been shown that this method can be used
for oilseed products, dried fruits and derived products.
2 Normat
...