ASTM E1510-95

NOTICE: This standard has either been superseded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E 1510 – 95
Standard Practice for
Installing Fused Silica Open Tubular Capillary Columns in
1
Gas Chromatographs
This standard is issued under the fixed designation E 1510; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
TABLE 1 Typical Splitter Vent Flow Rates (50 to 1 split ratio)
1. Scope
(at optimum linear velocity)
1.1 This practice is intended to serve as a general guide for
0.25-mm ID, 0.32-mm ID, 0.53-mm ID,
Carrier gas
the installation and maintenance of fused silica capillary
3 3 3
cm /min cm /min cm /min
columns in gas chromatographs which are already retrofitted helium 35 80 125
hydrogen 70 160 250
for their use. This practice excludes information on:
1.1.1 Injection techniques.
1.1.2 Column selection.
4
CGA P-9 The Inert Gases: Argon, Nitrogen and Helium
1.1.3 Data acquisition.
CGA V-7 Standard Method of Determining Cylinder Valve
1.1.4 System troubleshooting and maintenance.
4
Outlet Connections for Industrial Gas Mixtures
1.2 For additional information on gas chromatography,
4
CGA P-12 Safe Handling of Cryogenic Liquids
please refer to Practice E 260. For specific precautions, see
4
HB-3 Handbook of Compressed Gases
Notes 1-4.
1.3 This standard does not purport to address all of the
3. Terminology
safety concerns, if any, associated with its use. It is the
3.1 Terms and relations are defined in Practice E 355.
responsibility of the user of this standard to establish appro-
3.2 Nomenclature for open tubular or capillary columns
priate safety and health practices and determine the applica-
with a bore of 0.75 mm or less:
bility of regulatory limitations prior to use. For specific safety
3.3 porous layer open tubular (PLOT)—refers to columns
2
information see Section 6 and Notes 2-4.
with particles attached on the inside wall consisting of copoly-
mers such as styrene/divinylbenzene, molecular sieves, or
2. Referenced Documents
adsorbents such as Al O in film thicknesses of 5 to 50 μm.
2 2
2.1 ASTM Standards:
3.4 support coated open tubular (SCOT)—refers to fine
3
E 260 Practice for Packed Column Gas Chromatography
particles (silica or fine diatomite) coated with liquid stationary
E 355 Practice for Gas Chromatography Terms and Rela-
phase which is then deposited on the inside column wall to
3
tionships
improve stationary phase stability and sample capacity.
E 516 Practice for Testing Thermal Conductivity Detectors
3.5 wall coated open tubular (WCOT)—refers to columns
3
Used in Gas Chromatography
coated on the inside wall with a liquid stationary phase in film
E 594 Practice for Testing Flame Ionization Detectors Used
3
in Gas Chromatography
E 697 Practice for Use of Electron–Capture Detectors Used
3
in Gas Chromatography
2.2 CGA Publications:
CGA P-1 Safe Handling of Compressed Gases in Contain-
4
ers
CGA G-5.4 Standard for Hydrogen Piping Systems at Con-
4
sumer Locations
1
This practice is under the jurisdiction of ASTM Committee E13 on Molecular
Spectroscopy and is the direct responsibility of Subcommittee E13.19 on Chroma-
tography.
NOTE 1—The curves were generated by plotting the height equivalent
Current edition approved May 15, 1995. Published July 1995. Originally
to a theoretical plate (length of column divided by the total number of
published as E 1510 – 93. Last previous edition E 1510 – 93.
2 theoretical plates, H.E.T.P.) against the column’s average linear velocity.
Reprinted by permission of Restek Corp., 110 Benner Circle, Bellefonte, PA
The lowest point on the curve indicates the carrier gas velocity in which
16823-8812.
3
the highest column efficiency is reached.
Annual Book of ASTM Standards, Vol 14.02.
4
Available from Compressed Gas Association, Inc., 1725 Jefferson Davis FIG. 1 Van Deemter Profile for Hydrogen, Helium, and Nitrogen
4
Highway, Arlington, VA 22202-4100. Carrier Gases
Copyright © ASTM, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959, United States.
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E 1510
Carrier gas: Hydrogen Carrier gas: Helium
Linear velocity: 40 cm/s Linear velocity: 20 cm/s
NOTE 1—Septum bleed can obscure or co-elute with compounds of
NOTE 1—Fig. 2 shows that the resolution is similar but the analysis time
interest, thus decreasing the analytical accuracy.
is reduced by 50 % when comparing hydrogen to helium in an isothermal
NOTE 2—
analysis using optimum flow velocities.
1. 2,4,5,6-tetrachloro- 8. Heptachlor 16. p,p-DDD
NOTE 2—Hydrogen provides similar resolution in one-half the analysis
m-xylene (IS) epoxide 17. Endrin aldehyde
time of helium for an isothermal analysis.
2. a-BHC 9. g-chlordane 18. Endosu
...