SIST EN ISO 16094-2:2025
(Main)Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy methods for waters with low content of suspended solids including drinking water (ISO 16094-2:2025)
Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy methods for waters with low content of suspended solids including drinking water (ISO 16094-2:2025)
This document establishes key principles for the investigation of microplastics in drinking water and water with low content of natural suspended solids using a microscopy technique coupled with vibrational spectroscopy.
This method is applicable to:
— determine the size of microplastics [which range from 1 µm to 5 000 µm], count them and classify them by size range;
— identify the chemical composition of microplastics, the main ones (most used in industry and most abundant in the environment) being: polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polycarbonate (PC), polystyrene (PS), polytetrafluoroethylene (PTFE), polyvinyl chloride (PVC), polyamide (PA), polymethyl methacrylate (PMMA) and polyurethane (PU);
This method is applicable to water with a low content of organic matter and other suspended matter as defined in ISO 6107 (1 mg/l to 100 mg/l or lower when interfering with the determination), i.e.,
— ultrapure water;
— water intended for human consumption;
— raw groundwaters.
Given the very low concentrations of microplastics usually present in these waters, special attention needs to be paid to potential sources of contamination during sample preparation.
This method is intended to determine and characterize large numbers of particles in the sample in automatic mode.
This method can also identify the nature of the other particles that are outside the scope of this document, for example minerals, proteins, cellulose and pigments.
This method does not apply to the characterization of substances intentionally added to or adsorbed on the surface of microplastics. This method does not apply to the determination of the geometric shape of microplastics.
Wasserbeschaffenheit - Analyse von Kunststoff in Wasser - Teil 2: Verfahren mittels Vibrationsspektroskopie (ISO 16094-2:2025)
Dieses Dokument legt wichtige Grundsätze für die Untersuchung von Mikrokunststoffen in Trinkwasser und Wasser mit einem geringen Gehalt an natürlichen Schwebstoffen unter Verwendung eines Mikroskopieverfahrens in Verbindung mit der Schwingungsspektroskopie fest.
Dieses Verfahren ist anwendbar für:
die Ermittlung der Größe von Mikrokunststoffen [die von 1 µm bis 5 000 µm reichen], ihre Zählung sowie die Klassifizierung nach Größenbereichen;
die Bestimmung der chemischen Zusammensetzung von Mikrokunststoffen, wobei die wichtigsten (in der Industrie am meisten verwendeten und in der Umwelt am häufigsten vorkommenden) Mikrokunststoffe Polyethylen (PE), Polypropylen (PP), Polyethylenterephthalat (PET), Polycarbonat (PC), Polystyrol (PS), Polytetrafluorethylen (PTFE), Polyvinylchlorid (PVC), Polyamid (PA), Polymethylmethacrylat (PMMA) und Polyurethan (PU) sind.
Dieses Verfahren ist anwendbar auf Wasser mit einem geringen Gehalt an organischen Stoffen und anderen Schwebstoffen wie in ISO 6107 definiert (1 100 mg/l oder weniger, falls sie die Ermittlung stören), d. h.,
Reinstwasser;
Wasser für den menschlichen Gebrauch;
rohes Grundwasser.
In Anbetracht der sehr geringen Konzentrationen von Mikrokunststoffen, die in diesen Wässern üblicherweise vorhanden sind, ist es erforderlich, bei der Probenvorbereitung besonders auf mögliche Kontaminationsquellen zu achten.
Dieses Verfahren ist für die Ermittlung und Charakterisierung einer großen Anzahl von Partikeln in der Probe im automatischen Modus vorgesehen.
Mit diesem Verfahren kann auch die Beschaffenheit der sonstigen Partikel, die nicht in den Anwendungsbereich dieses Dokuments fallen, z. B. Mineralien, Proteine, Zellulose und Pigmente, bestimmt werden.
Dieses Verfahren gilt nicht für die Charakterisierung von Stoffen, die absichtlich Mikrokunststoffen zugesetzt oder an deren Oberfläche adsorbiert wurden. Dieses Verfahren gilt nicht für die Ermittlung der geometrischen Form von Mikrokunststoffen.
Qualité de l'eau - Analyse des microplastiques dans l'eau - Partie 2: Méthodes de spectroscopie vibrationnelle pour les eaux à faible teneur en matières en suspension, y compris l'eau potable (ISO 16094-2:2025)
Le présent document établit les principes clés de l'étude des microplastiques dans l'eau potable et les eaux à faible teneur en matières en suspension naturelles à l'aide d'une technique de microscopie couplée à une spectroscopie vibrationnelle.
Cette méthode est applicable pour:
— déterminer la taille des microplastiques [qui va de 1 µm à 5 000 µm], les dénombrer et les classer par intervalles de tailles;
— identifier la composition chimique des microplastiques, dont les principaux (les plus utilisés dans l'industrie et les plus présents dans l'environnement) sont les Polyéthylène (PE), Polypropylène (PP), Poly(téréphtalate d'éthylène) (PET), Polycarbonate (PC), Polystyrène (PS), Polytétrafluoroéthylène (PTFE), Polychlorure de vinyle (PVC), Polyamide (PA), Polyméthacrylate de méthyle (PMMA) et Polyuréthane (PU);
Cette méthode est applicable aux eaux à faible teneur en matière organique et en autres matières en suspension comme défini dans l'ISO 6107 (1 mg/l à 100 mg/l ou moins lorsque cela interfère avec la détermination), notamment:
— les eaux ultra pures;
— les eaux destinées à la consommation humaine;
— les eaux souterraines brutes.
Étant donné les très faibles concentrations de microplastiques habituellement présentes dans ces eaux, une attention particulière doit être portée aux possibles sources de contamination lors de la préparation de l'échantillon.
Cette méthode a pour but de déterminer et de caractériser un grand nombre de particules dans l'échantillon en mode automatique.
Cette méthode peut aussi identifier la nature des autres particules qui n'entrent pas dans le domaine d'application du présent document, par exemple les minéraux, les protéines, la cellulose et les pigments.
Cette méthode ne s'applique pas à la caractérisation des substances intentionnellement ajoutées ou adsorbées à la surface des microplastiques. Cette méthode ne s'applique pas à la détermination de la forme géométrique de microplastiques.
Kakovost vode - Analiza mikroplastike v vodi - 2. del: Metode vibracijske spektroskopije za vodo z nizko vsebnostjo suspendiranih delcev, vključno s pitno vodo (ISO 16094-2:2025)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2025
Kakovost vode - Analiza mikroplastike v vodi - 2. del: Metode vibracijske
spektroskopije za vodo z nizko vsebnostjo suspendiranih delcev, vključno s pitno
vodo (ISO 16094-2:2025)
Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy
methods for waters with low content of suspended solids including drinking water (ISO
16094-2:2025)
Wasserbeschaffenheit - Analyse von Kunststoff in Wasser - Teil 2: Verfahren mittels
Vibrationsspektroskopie (ISO 16094-2:2025)
Qualité de l'eau - Analyse des microplastiques dans l'eau - Partie 2: Méthodes de
spectroscopie vibrationnelle pour les eaux à faible teneur en matières en suspension, y
compris l'eau potable (ISO 16094-2:2025)
Ta slovenski standard je istoveten z: EN ISO 16094-2:2025
ICS:
13.060.45 Preiskava vode na splošno Examination of water in
general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 16094-2
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2025
EUROPÄISCHE NORM
ICS 13.060.45
English Version
Water quality - Analysis of microplastic in water - Part 2:
Vibrational spectroscopy methods for waters with low
content of suspended solids including drinking water (ISO
16094-2:2025)
Qualité de l'eau - Analyse des microplastiques dans Wasserbeschaffenheit - Analyse von Kunststoff in
l'eau - Partie 2: Méthodes de spectroscopie Wasser - Teil 2: Verfahren mittels
vibrationnelle pour les eaux à faible teneur en matières Vibrationsspektroskopie (ISO 16094-2:2025)
en suspension, y compris l'eau potable (ISO 16094-
2:2025)
This European Standard was approved by CEN on 14 September 2025.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16094-2:2025 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 16094-2:2025) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2026, and conflicting national standards shall be
withdrawn at the latest by April 2026.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 16094-2:2025 has been approved by CEN as EN ISO 16094-2:2025 without any
modification.
International
Standard
ISO 16094-2
First edition
Water quality — Analysis of
2025-09
microplastic in water —
Part 2:
Vibrational spectroscopy methods
for waters with low content
of suspended solids including
drinking water
Qualité de l'eau — Analyse des microplastiques dans l'eau —
Partie 2: Méthodes de spectroscopie vibrationnelle pour les eaux
à faible teneur en matières en suspension, y compris l'eau potable
Reference number
ISO 16094-2:2025(en) © ISO 2025
ISO 16094-2:2025(en)
© ISO 2025
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii
ISO 16094-2:2025(en)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 2
3 Terms, definitions and abbreviations . 2
3.1 Terms and definitions .2
3.2 Abbreviations .3
4 Principle . 4
5 Interferences . 4
6 Reagents, consumables and reference materials . 4
6.1 General .4
6.2 Particle-free water of known quality .4
6.3 Surfactant solution .5
6.4 Ethanol .5
6.5 Plastic reference material . .5
6.6 Containers for filter storage .5
7 Precautions for the laboratory environment, equipment and materials . 5
7.1 Operating precautions linked to the laboratory environment .5
7.2 Equipment .5
7.2.1 General .5
7.2.2 Infrared (IR) apparatus coupled to microscope.5
7.2.3 Raman coupled to microscope .6
7.2.4 Filtration system .6
7.2.5 Characteristics of filters: type and sizes.7
7.3 Precautions and cleaning protocol for materials .7
7.4 Bottles or containers for sampling .7
8 Sampling . 8
9 Operating protocol . 8
9.1 Volume of test sample .8
9.2 Sample filtration protocol .8
9.3 Analytical control blanks .9
9.4 Adjustment and calibration of instruments .9
9.4.1 Infrared microscope .9
9.4.2 Raman microscope .9
9.5 Cartesian coordinates of analysed particles.10
9.6 Analysis of the filter surface .10
9.6.1 General .10
9.6.2 Choice of the optical objectives .10
9.6.3 Selection of particles to be analysed or choice of the analysed surface area.10
9.6.4 TOTAL Model .10
9.6.5 RANDOM Model.
...
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