EN ISO 6579-1:2017
(Main)Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. (ISO 6579-1:2017)
Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. (ISO 6579-1:2017)
ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling;
- samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis, zur Zählung und zur Serotypisierung von Salmonellen - Teil 1: Nachweis von Salmonella spp. (ISO 6579-1:2017)
Dieses Dokument legt ein horizontales Verfahren zum Nachweis von Salmonellen fest. Es gilt für Folgendes:
- Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind;
- Umgebungsproben im Bereich der Herstellung von Lebensmitteln und beim Umgang mit Lebensmitteln;
- Proben aus der Primärproduktion, wie Tierkot, Staub und Tupfer.
Mit diesem horizontalen Verfahren sollen die meisten der Salmonella Serovare nachgewiesen werden. Für den Nachweis einiger spezifischer Serovare können zusätzliche Kultivierungsschritte notwendig sein. Für Salmonella Typhi und Salmonella Paratyphi ist die Durchführung in Anhang D beschrieben.
Das selektive Anreicherungsmedium modifizierter halbfester Rappaport Vassiliadis(MSRV) Agar ist für den Nachweis von beweglichen Salmonellen vorgesehen und ist für den Nachweis von unbeweglichen Salmonellen Stämmen nicht geeignet.
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche, le dénombrement et le sérotypage des Salmonella - Partie 1: Recherche des Salmonella spp. (ISO 6579-1:2017)
ISO 6759-1:2017 spécifie une méthode horizontale de recherche des Salmonella. Il s'applique aux:
- produits destinés à la consommation humaine et à l'alimentation animale.
- échantillons environnementaux dans le domaine de la production et de la manutention de denrées alimentaires.
- échantillons au stade de la production primaire, tels que des matières fécales, de la poussière ou des prélèvements de surface.
Cette méthode horizontale vise à rechercher la plupart des sérovars de Salmonella. Des étapes de culture supplémentaires peuvent être nécessaires pour la recherche de certains sérovars spécifiques. Pour Salmonella Typhi et Salmonella Paratyphi, le mode opératoire est décrit en Annexe D.
Le milieu d'enrichissement sélectif MSRV, gélose de Rappaport‐Vassiliadis semi‐solide modifiée, est conçu pour la recherche des Salmonella mobiles et n'est pas adapté à la détection des Salmonella immobiles.
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella - 1. del: Ugotavljanje prisotnosti Salmonella spp. (ISO 6579-1:2017)
Ta dokument določa horizontalno metodo za ugotavljanje prisotnosti bakterij Salmonella. Uporablja se
za:
– izdelke, namenjene za prehrano ljudi in krmo živali;
– okoljske vzorce na območju proizvodnje hrane in ravnanja s hrano;
– vzorce primarne stopnje proizvodnje (npr. živalski iztrebki, prah in brisi).
S to horizontalno metodo se predvidoma ugotovi prisotnost večine serovarjev Salmonella. Za ugotavljanje
prisotnosti nekaterih posebnih serovarjev bodo morda potrebi dodatni koraki kulture. Postopek za bakterije Salmonella Typhi in Salmonella Paratyphi je opisan v dodatku D.
Medij selektivnega bogatenja, spremenjen poltrd agar Rappaport–Vassiliadis (MSRV), je namenjen za odkrivanje gibljivih sevov bakterij Salmonella in ni primeren za odkrivanje negibljivih sevov bakterij Salmonella.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2017
1DGRPHãþD
SIST EN ISO 6579:2003
SIST EN ISO 6579:2003/A1:2007
SIST EN ISO 6579:2003/AC:2004
SIST EN ISO 6579:2003/AC:2006
SIST EN ISO 6785:2007
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti, števila in serotipov Salmonella - 1. del: Ugotavljanje prisotnosti
Salmonella spp. (ISO 6579-1:2017)
Microbiology of the food chain - Horizontal method for the detection, enumeration and
serotyping of Salmonella - Part 1: Detection of Salmonella spp. (ISO 6579-1:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis, zur
Zählung und zur Serotypisierung von Salmonellen - Teil 1: Nachweis von Salmonella
spp. (ISO 6579-1:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche, le
dénombrement et le sérotypage des Salmonella - Partie 1: Recherche des Salmonella
spp. (ISO 6579-1:2017)
Ta slovenski standard je istoveten z: EN ISO 6579-1:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 6579-1
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 6579:2002, EN ISO 6785:2007
English Version
Microbiology of the food chain - Horizontal method for the
detection, enumeration and serotyping of Salmonella - Part
1: Detection of Salmonella spp. (ISO 6579-1:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche, le dénombrement et le Verfahren zum Nachweis, zur Zählung und zur
sérotypage des Salmonella - Partie 1: Recherche des Serotypisierung von Salmonellen - Teil 1: Nachweis
Salmonella spp. (ISO 6579-1:2017) von Salmonella spp. (ISO 6579-1:2017)
This European Standard was approved by CEN on 3 February 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6579-1:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 6579-1:2017) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN, in collaboration with Technical
Committee ISO/TC 34 "Food products".
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2017 and conflicting national standards
shall be withdrawn at the latest by September 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 6579:2002 and EN 6785:2007.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands,
Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
the United Kingdom.
Endorsement notice
The text of ISO 6579-1:2017 has been approved by CEN as EN ISO 6579-1:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 6579-1
First edition
2017-02
Microbiology of the food chain —
Horizontal method for the detection,
enumeration and serotyping of
Salmonella —
Part 1:
Detection of Salmonella spp.
Microbiologie de la chaîne alimentaire — Méthode horizontale
pour la recherche, le dénombrement et le sérotypage des
Salmonella —
Partie 1: Recherche des Salmonella spp.
Reference number
ISO 6579-1:2017(E)
©
ISO 2017
ISO 6579-1:2017(E)
© ISO 2017, Published in Switzerland
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ISO 6579-1:2017(E)
Contents Page
Foreword .v
Introduction .vii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Pre-enrichment in non-selective liquid medium . 2
4.3 Enrichment in/on selective media . 2
4.4 Plating out on selective solid media . 2
4.5 Confirmation . 3
5 Culture media, reagents, and antisera . 3
6 Equipment and consumables . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure (see diagrams in Annex A) . 4
9.1 Test portion and initial suspension . 4
9.2 Non-selective pre-enrichment . 4
9.3 Selective enrichment. 5
9.3.1 General. 5
9.3.2 Procedure for food, animal feed samples, and environmental samples
from the food production area . 5
9.3.3 Procedure for samples from the primary production stage . 5
9.4 Plating out . 6
9.4.1 General. 6
9.4.2 Procedure for food, animal feed samples, and environmental samples
from the food production area . 6
9.4.3 Procedure for samples from the primary production stage . 6
9.5 Confirmation . 7
9.5.1 General. 7
9.5.2 Selection of colonies for confirmation . 7
9.5.3 Biochemical testing . 8
9.5.4 Serological testing . . .11
9.5.5 Interpretation of biochemical and serological reactions .11
9.5.6 Serotyping.12
10 Expression of results .12
11 Performance characteristics of the method .12
11.1 Interlaboratory studies .12
11.2 Sensitivity .12
11.3 Specificity .12
11.4 LOD .
50 12
12 Test report .13
Annex A (normative) Diagrams of the procedures .
...
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