EN ISO 11212-1:1997
(Main)Starch and derived products - Heavy metals content - Part 1: Determination of arsenic content by atomic absorption spectrometry (ISO 11212-1:1997)
Starch and derived products - Heavy metals content - Part 1: Determination of arsenic content by atomic absorption spectrometry (ISO 11212-1:1997)
Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11212).
Stärke und Stärkederivate - Schwermetallgehalt - Teil 1: Bestimmung des Arsengehaltes durch Atomabsorptionsspektrometrie (ISO 11212-1:1997)
Dieser Teil von ISO 11212 legt ein Verfahren für die Bestimmung des Arsengehaltes von Stärke, einschließlich von Derivaten und Nebenprodukten, durch Atomabsorptionsspektrometrie mit Hydriderzeugung fest. Die gegenwärtig verfügbaren Hydriderzeuger verwenden sehr verschiedene Verfahren; es ist folglich unmöglich, ein umfassendes Verfahren vorzuschlagen, das das Erreichen von zufriedenstellenden Ergebnissen für alle Gerätetypen sicherzustellt.
Amidons, fécules et produits dérivés - Teneur en métaux lourds - Partie 1: Détermination de la teneur en arsenic par spectrométrie d'absorption atomique (ISO 11212-1:1997)
La présente partie de l'ISO 11212 prescrit une méthode pour la détermination de la teneur en arsenic dans les amidons, fécules, produits dérivés et sous-produits d'amidonnerie par spectrométrie d'absorption atomique avec génération d'hydrures. Les appareils générateurs d'hydrures actuellement commercialisés utilisant des techniques très différentes, il est donc impossible de proposer une méthode détaillée susceptible de garantir l'obtention de résultats satisfaisants sur tous les types d'appareils. Il convient donc que chaque analyste procède à l'optimisation des conditions d'utilisation de son propre instrument sur la base des recommandations générales ou particulières qui lui sont données.
Škrob in škrobni derivati - Delež težkih kovin - 1. del: Določevanje arzena z atomsko absorpcijsko spektrometrijo (ISO 11212-1:1997)
General Information
Relations
Overview
EN ISO 11212-1:1997 is an international standard developed by CEN in alignment with ISO guidelines. It specifies a reliable analytical method for the determination of arsenic content in starch and derived products using atomic absorption spectrometry (AAS) with hydride generation. This standard ensures accurate monitoring of heavy metals content in starch materials, which is critical for food safety, regulatory compliance, and quality control.
The method focuses on quantifying arsenic (As) in micrograms per kilogram (μg/kg) through a combination of sample digestion, hydride generation, and spectrometric measurement at a wavelength of 193.7 nm. It applies to starch, its derivatives, and by-products, enhancing safety standards across diverse industrial applications.
Key Topics
- Scope: Determines arsenic concentration in starch products using atomic absorption spectrometry.
- Principle:
- Wet digestion of organic matrices with nitric acid and hydrogen peroxide.
- Reduction of arsenic ions (As³⁺) to arsine gas via sodium borohydride in acidic medium.
- Detection using atomic absorption spectrometry with hydride generation at 193.7 nm wavelength.
- Reagents and Apparatus:
- Use of high-purity reagents such as nitric acid, sodium borohydride, and arsenic standard solutions.
- Borosilicate glass digestion equipment and a specialized hydride generator.
- High-resolution AAS equipment with arsenic hollow cathode lamp.
- Procedure:
- Homogenization of test samples followed by reflux digestion.
- Calibration using standard arsenic solutions for accuracy.
- Measurement of sample absorbance with blank correction.
- Precision and Validation:
- Defines repeatability and reproducibility limits based on interlaboratory studies.
- Quantification limit reaches as low as 20 μg/kg, supporting detection of trace arsenic levels.
- Test Reporting:
- Requires comprehensive documentation including sampling methods, test results, and any procedural deviations.
Applications
- Food Industry: Ensures safety and compliance by quantifying toxic arsenic levels in starch-based food products and ingredients.
- Quality Control Labs: Enables precise arsenic monitoring during starch production and processing for risk mitigation.
- Regulatory Compliance: Assists manufacturers in meeting international heavy metals limits in foodstuffs and additives.
- Research and Development: Supports product testing and formulation involving starch derivatives with focus on contaminant assessment.
- Environmental Monitoring: Facilitates analysis of heavy metals in agricultural starch raw materials and by-products.
Related Standards
EN ISO 11212 forms part of a series addressing heavy metals in starch and derived products, including:
- Part 2: Determination of mercury content by atomic absorption spectrometry
- Part 3: Determination of lead content by atomic absorption spectrometry with electrothermal atomization
- Part 4: Determination of cadmium content by atomic absorption spectrometry with electrothermal atomization
These complementary standards provide a comprehensive framework for heavy metals testing, ensuring holistic safety evaluation of starch products.
Keywords: EN ISO 11212-1:1997, arsenic determination, starch heavy metals testing, atomic absorption spectrometry, hydride generation AAS, food safety standards, starch derivatives arsenic content, heavy metals analysis, arsenic quantification method, analytical chemistry starch products
Frequently Asked Questions
EN ISO 11212-1:1997 is a standard published by the European Committee for Standardization (CEN). Its full title is "Starch and derived products - Heavy metals content - Part 1: Determination of arsenic content by atomic absorption spectrometry (ISO 11212-1:1997)". This standard covers: Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11212).
Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11212).
EN ISO 11212-1:1997 is classified under the following ICS (International Classification for Standards) categories: 67.180 - Sugar. Sugar products. Starch. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 11212-1:1997 has the following relationships with other standards: It is excused to EN ISO 11212-2:1997, EN ISO 11212-4:1997, EN ISO 11212-3:1997. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase EN ISO 11212-1:1997 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-1998
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DWRPVNRDEVRUSFLMVNRVSHNWURPHWULMR ,62
Starch and derived products - Heavy metals content - Part 1: Determination of arsenic
content by atomic absorption spectrometry (ISO 11212-1:1997)
Stärke und Stärkederivate - Schwermetallgehalt - Teil 1: Bestimmung des Arsengehaltes
durch Atomabsorptionsspektrometrie (ISO 11212-1:1997)
Amidons, fécules et produits dérivés - Teneur en métaux lourds - Partie 1: Détermination
de la teneur en arsenic par spectrométrie d'absorption atomique (ISO 11212-1:1997)
Ta slovenski standard je istoveten z: EN ISO 11212-1:1997
ICS:
67.180.20 Škrob in izdelki iz njega Starch and derived products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
INTERNATIONAL
STANDARD
First edition
1997-03-I 5
Starch and derived products - Heavy
metals content -
Part 1:
Determination of arsenic content by atomic
absorption spectrometry
Amidons, f&u/es et produits d&iv& - Teneur en mktaux lourds -
Partie 1: Ddtermination de la teneur en arsenic par spectromktrie
d ’absorption atomique
Reference number
IS0 11212-1:1997(E)
IS0 11212-1:1997(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work of
preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented
on that committee. International organizations, governmental and non-
governmental, in liaison with ISO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard IS0 11212-l was prepared by Technical Committee
ISOmC 93, Starch (including derivatives and by-products).
IS0 11212 consists of the following parts, under the general title Starch
and derived products - Heavy metals content.
- Pall 1: Determination of arsenic content by atomic absorption
spectrometry
- Part 2: Determination of mercury content by atomic absorption
spectrometry
- Part 3: Determination of lead content by atomic absorption
spectrometry with electrothermal atomization
- Pan 4: Determination of cadmium content by atomic absorption
spectrometry with electrothermal atomization
Annex A of this part of IS0 11212 is for information only.
0 IS0 1997
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-1211 Geneve 20 l Switzerland
Internet central @ iso.ch
x.400
c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii
IS0 11212=1:1997(E)
INTERNATIONAL STANDARD @ Iso
Starch and derived products - Heavy metals content -
Part 1:
Determination of arsenic content by atomic absorption spectrometry
1 Scope
This part of IS0 11212 specifies a method for the determination of the arsenic content of starch, including
derivatives and by-products, by atomic absorption spectrometry with hybride generation.
The hybride generators currently available use very different techniques; it is thus impossible to propose a
comprehensive method likely to ensure the attainment of satisfactory results on all types of apparatus. Each analyst
should therefore optimize the conditions of use of his/her own apparatus on the basis of general or particular
instructions.
2 Definition
For the purposes of this part of IS0 11212, the following definition applies.
2.1 arsenic content: Quantity of arsenic determined in accordance with the conditions specified in this method
and expressed as arsenic (As), in micrograms per kilogram of the product as received.
3 Principle
Wet digestion of the organic matrix. Reduction of arsenic (As3+) to arsenic hydride by hydrogen resulting from the
action of sodium borohydride on hydrochloric acid. Entrainment of the hydride form by a flow of gas and
determination by atomic absorption spectrometry in a heated quartz cell.
Measurement of the absorbance at a wavelength of 193,7 nm.
Determination of the concentration of arsenic in the sample by means of a calibration curve.
4 Reagents
Use only reagents’ of recognized analytical grade and distilled water or water of equivalent purity.
4.1 Nitric acid (pzO = I,38 g/ml).
4.2 Hydrogen peroxide, 30 % (VW) solution.
@ IS0
IS0 11212=1:1997(E)
4.3 Sodium borohydride solution
Prepare a solution at the concentration recommended in the instructions for use of the hydride generator (5.3).
4.4 Hydrochloric acid solution
Prepare a solution at the concentration recommended in the instructions for use of the hydride generator (5.3).
4.5 Arsenic standard solution, 1 g/l.
Standard solutions are commercially available at this concentration. These solutions may be prepared by weighing
and dissolving the salt or metal of known purity.
4.6 Calibration solutions
Before each series of measurements, prepare from the standard arsenic solution (4.5) at least five calibration
solutions covering the range of concentrations to be determined. 100 ml of each calibration solution shall contain
73 ml of nitric acid (4.1).
5 Apparatus
All the glassware used shall be previously washed by means of suitable products (such as nitric acid) and rinsed
with distilled water to eliminate any trace of arsenic.
Use ordinary laboratory apparatus and, in particular, the following.
5.1 Digestion apparatus (see figure I), made of borosilicate glass and consisting of three elements terminating
with conical ground joints (5.1 .I to 5.1.3).
5.1.1 Soxhlet extraction tube, of capacity 200 ml, equipped with a stopcock and a lateral tube connected directly
to the flask (5.1.3).
51.2 Cooling apparatus, 35 cm long, con
...
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