CEN/SS C10 - Starch

ISO 10504:2013 describes a high-performance liquid chromatographic (HPLC) method for measuring the composition of dextrose solutions, glucose syrups, fructose-containing syrups, hydrogenated glucose syrups, sorbitol, mannitol and maltitol. The constituents are mainly glucose, maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto-oligosaccharides.
The use of a column packed with cation-exchange resin is essential.

VA/ISO removed because ISO already published the document in 2000. CMC will publish it as a CEN publication (CC/020211)

Migrated from Progress Sheet (TC Comment) (2000-07-10): UAP to be launched on this WI as soon as ISO 10520 is published. ++ gdj/1996_05_14

Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11215).

Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11216).

Gives a method for the determination of the moisture content of native or modified starch using oven-drying at 130 °C under atmospheric pressure.

Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11214).

Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11212).

Applies to modified starch, both granular and soluble in cold water. The methods specified enable to measure total and free acetyl contents; bound acetyl content is calculated as the difference of the two values. Total content is determined by heating the sample with dilute hydrochloric acid and converting the acetate with adenosine-5-triphosphate (ATP) and coenzyme A (CoA) to acetyl-CoA. The methods are suitable for determining acetyl contents up to 2 % (m/m).

The method is applicable to the anhydrous product and to the monohydrat. It consists in drying of a test portion at 100 °C and a pressure not exceeding 135 mbar.

Hydrolysis of the product by boiling hydrochloric acid, and coagulation of the insoluble products by cooling. Separation by filtration, drying and isolation the wanted matter by solvent extraction. The method applies to contents less than 1,5 % (m/m).

The found value only includes free amino acids, compounds producing amino acids on hydrolysis and ammonium compounds. The principle consists in destruction of organic matter by sulphuric acid in the presence of a compound catalyst (see ISO 1871), alkalization of the reaction products, distillation of the liberated ammonia and collection in a boric acid solution, followed by titration with a standard volumetric sulphuric acid solution. The method applies to contents greater than 0,01 % (m/m). For products with contents less than 0,025 % (m/m), see ISO 5378.

Destruction of organic matter by sulphuric acid in the presence of a compound catalyst (see ISO 1871), alkalization of the reaction products, destillation of the liberated ammonia and collection in a sulphuric acid solution, followed by measuring of the ammonium salt formed after the addition of Nessler reagent. The method is provided for contents less than 0,025 % (m/m). For greater contents, see ISO 3188.

Destruction of the organic substances by digestion with a sulpho-nitric mixture and conversion of phosphates to orthophosphates. Formation of a phosphomolybdate. Measurement of the intensity of the blue colour at a wavelength of 825 nm. The method applies to contents up to 5 % (m/m).

Titration of a prescribed volume of mixed Fehling's solution with a solution of a test portion under specified conditions, using methylene blue as internal indicator. An informative annex provides a metod for testing standard reference anhydrous D-glucose, necessary for the procedure.

Reaction of a solution of iodine, sulphur dioxide, pyridine and 2-methoxyethanol with the water contained in the product dispersed previously in a mixture of methanol and formamide. The method described in ISO 760 is improved by determing the content directly using the special solvent.

Incineration of a test portion at a temperature of 900 °C, until complete disappearance of the carbon in the residue. The method specifies a procedure for the evaluation of natural and added mineral substances together. It applies to contents up to 2 %. It does not apply to hydrolysis products nor to oxidized starches, nor to other products containing more than 0,2 % of chloride. In other cases, see ISO 5809.