EN ISO 17234-1:2015

SLOVENSKI STANDARD
01-junij-2015
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SIST EN ISO 17234-1:2011
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Leather - Chemical tests for the determination of certain azo colorants in dyed leathers -
Part 1: Determination of certain aromatic amines derived from azo colorants (ISO 17234-
1:2015)
Leder - Chemische Prüfungen zur Bestimmung bestimmter Azofarbstoffe in gefärbten
Ledern - Teil 1: Bestimmung bestimmter aromatischer Amine aus Azofarbstoffen (ISO
17234-1:2015)
Cuir - Essais chimiques pour le dosage de certains colorants azoïques dans les cuirs
teints - Partie 1: Dosage de certaines amines aromatiques dérivées des colorants
azoïques (ISO 17234-1:2015)
Ta slovenski standard je istoveten z: EN ISO 17234-1:2015
ICS:
59.140.30 Usnje in krzno Leather and furs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 17234-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2015
ICS 59.140.30 Supersedes EN ISO 17234-1:2010
English Version
Leather - Chemical tests for the determination of certain azo
colorants in dyed leathers - Part 1: Determination of certain
aromatic amines derived from azo colorants (ISO 17234-1:2015)
Cuir - Essais chimiques pour le dosage de certains Leder - Chemische Prüfungen zur Bestimmung bestimmter
colorants azoïques dans les cuirs teints - Partie 1: Dosage Azofarbstoffe in gefärbten Ledern - Teil 1: Bestimmung
de certaines amines aromatiques dérivées des colorants bestimmter aromatischer Amine aus Azofarbstoffen (ISO
azoïques (ISO 17234-1:2015) 17234-1:2015)
This European Standard was approved by CEN on 14 February 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17234-1:2015 E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (EN ISO 17234-1:2015) has been prepared by IULTCS “International Union of Leather
Technologists and Chemists Societies" in collaboration with Technical Committee CEN/TC 289 “Leather” the
secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by October 2015, and conflicting national standards shall be withdrawn at
the latest by October 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 17234-1:2010.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 17234-1:2015 has been approved by CEN as EN ISO 17234-1:2015 without any modification.

INTERNATIONAL ISO
STANDARD 17234-1
IULTCS/IUC 20-1
Second edition
2015-04-01
Leather — Chemical tests for the
determination of certain azo colorants
in dyed leathers —
Part 1:
Determination of certain aromatic
amines derived from azo colorants
Cuir — Essais chimiques pour le dosage de certains colorants
azoïques dans les cuirs teints —
Partie 1: Dosage de certaines amines aromatiques dérivées des
colorants azoïques
Reference numbers
ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
©
ISO 2015
ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
© ISO 2015
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2015 – All rights reserved

ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 General . 1
4 Principle . 2
5 Safety precautions . 3
6 Apparatus . 3
7 Reagents . 4
8 Sampling and preparation of samples . 5
9 Procedure. 5
9.1 Degreasing . 5
9.2 Reductive cleavage . 5
9.3 Liquid-liquid extraction . 5
9.4 Check of the analytical system . 6
10 Chromatographic analyses . 6
11 Calibration . 6
12 Evaluation . 6
12.1 Calculation of amine in the sample . 6
12.2 Reliability of the method . 7
13 Test report . 7
Annex A (informative) Chromatographic analyses . 8
Annex B (informative) Reliability of the method .12
Annex C (informative) Assessment guide — Interpretation of analytical results .13
Bibliography .15
ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
ISO 17234-1 was prepared by the Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the Agreement on technical cooperation between ISO and CEN
(Vienna Agreement). This method is technically similar to the method in IUC 20, which was declared an
official method at the IULTCS Delegates meeting on 31st May 2003 in Cancun, Mexico.
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three Commissions, which are
responsible for establishing international methods for the sampling and testing of leather. ISO recognizes
IULTCS as an international standardizing body for the preparation of test methods for leather.
This second edition of ISO 17234-1 cancels and replaces the first edition (ISO 17234-1:2010), which
has been technically revised to include two new aromatic amines from the Chinese Standard GB 20400
in Table 1. The layout for this part of ISO 17234 has been re-arranged and updated to be the same as
ISO 17234-2.
ISO 17234 consists of the following parts, under the general title Leather — Chemical tests for the
determination of certain azo colorants in dyed leathers:
— Part 1: Determination of certain aromatic amines derived from azo colorants
— Part 2: Determination of 4-aminoazobenzene
iv © ISO 2015 – All rights reserved

INTERNATIONAL STANDARD ISO 17234-1:2015(E)
Leather — Chemical tests for the determination of certain
azo colorants in dyed leathers —
Part 1:
Determination of certain aromatic amines derived from
azo colorants
1 Scope
This part of ISO 17234 specifies a method for determining the use of certain azo colorants which can
release certain aromatic amines.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 2418, Leather — Chemical, physical and mechanical and fastness tests — Sampling location
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 4044, Leather — Chemical tests — Preparation of chemical test samples
ISO 17234-2, Leather — Chemical tests for the determination of certain azo colorants in dyed leathers —
Part 2: Determination of 4-aminoazobenzene
3 General
Certain azo colorants can release, by reductive cleavage of azo group(s), one or more of the following
aromatic amines, which are listed in Appendix 8 of EU Regulation 1907/2006, Annex XVII of the European
Parliament, and/or the Chinese Standard GB 20400-2006.
Table 1 — Aromatic amines listed in Appendix 8 of EU regulation 1907/2006, Annex XVII,
and/or GB 20400–2006
No. CAS number Index number EC number Substances
1 92–67–1 612–072–00–6 202–177–1 biphenyl-4-ylamine
4-aminobiphenyl
xenylamine
2 92–87–5 612–042–00–2 202–199–1 benzidine
3 95–69–2 202–441–6 4-chloro-o-toluidine
4 91–59–8 612–022–00–3 202–080–4 2-naphthylamine
a
5 97–56–3 611–006–00–3 202–591–2 o-aminoazotoluene
4-amino-2’,3-dimethyla-
zobenzene
4-o-tolylazo-o-toluidine
a
6 99–55–8 202–765–8 5-nitro-o-toluidine
7 106–47–8 612–137–00–9 203–401–0 4-chloroaniline
ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
Table 1 (continued)
No. CAS number Index number EC number Substances
8 615–05–4 210–406–1 4-methoxy-m-phenylenedi-
amine
9 101–77–9 612–051–00–1 202–974–4 4,4’-methylenedianiline
4,4’-diaminodiphenylmeth-
ane
10 91–94–1 612–068–00–4 202–109–0 3,3’-dichlorobenzidine
3,3’-dichlorobiphenyl-4,4’-
ylenediamine
11 119–90–4 612–036–00-X 204–355–4 3,3’-dimethoxybenzidine
o-dianisidine
12 119–93–7 612–041–00–7 204–358–0 3,3’-dimethylbenzidine
4,4’-bi-o-toluidine
13 838–88–0 612–085–00–7 212–658–8 4,4’-methylenedi-o-tolui-
dine
14 120–71–8 204–419–1 6-methoxy-m-toluidine
p-cresidine
15 101–14–4 612–078–00–9 202–918–9 4,4’-methylene-bis-
(2-chloro-aniline)
2,2’-dichloro-4.4’-methyl-
ene-dianiline
16 101–80–4 202–977–0 4,4’-oxydianiline
17 139–65–1 205–370–9 4,4’-thiodianiline
18 95–53–4 612–091–00-X 202–429–0 o-toluidine
2-aminotoluene
19 95–80–7 612–099–00–3 202–453–1 4-methyl-m-phenylenedi-
amine
20 137–17–7 205–282–0 2,4,5-trimethylaniline
21 90–04–0 612–035–00–4 201–963–1 o-anisidine
2-methoxyaniline
b
22 60–09–3 611–008–00–4 200–453–6 4-aminoazobenzene
c
23 95–68–1 612–027–00–0 202–440–0 2,4-xylidine
2,4-dimethylbenzene-
1-amine
c
24 87–62–7 612–161–00-X 201–758–7 2,6-xylidine
2,6-dimethylbenzene-
1-amine
a
The CAS-numbers 97–56–3 (No. 5) and 99–55–8 (No. 6) are further reduced to CAS-numbers 95–53–4 (No.
18) and 95–80–7 (No. 19).
b
Azo colorants that are able to form 4-aminoazobenzene, generate under the condition of this method aniline
and/or 1,4-phenylenediamine. The presence of these colorants shall be tested using ISO 17234-2.
c
Additional aromatic amines in the Chinese Standard GB 20400–2006
4 Principle
After degreasing, the leather sample is treated with sodium dithionite in an aqueous buffer solution (pH
6) at 70 °C in a closed vessel. The amines released in the process of reductive cleavage are transferred to
a t-butyl methyl ether phase by means of liquid-liquid extraction using Kieselgur columns. The t-butyl
methyl ether extract is then concentrated under mild conditions in a rotary vacuum evaporator and the
residue is dissolved in a suitable solvent, depending on the method used to determine the amines.
2 © ISO 2015 – All rights reserved

ISO 17234-1:2015(E)
IULTCS/IUC 20-1:2015(E)
Determination of the amines is performed by means of high-peformance liquid chromatography (HPLC)
using a diode array detector (DAD) or mass selective detector (HPLC/MS), capillary gas chromatography
with a mass-selective detector (GC-MS) or by capillary electrophoresis with a diode array detector
(CE/DAD), or qualitatively with thin layer chromatography (TLC, HPTLC).
The amines shall be identified by means of at least two different chromatographic separation methods
in order to avoid any possible misinterpretations caused by interfering substances (such as position
isomers of the amines to be identified) and hence any incorrect statements. Amine quantification shall
be performed by HPLC/DAD or GC/MS.
5 Safety precautions
5.1 WARNING — The aromatic amines listed in Clause 3 are classified as substances known to be
or suspected to be human carcinogens.
Any handling and disposal of this substance shall be in strict accordance with the appropriate
national health and safety regulations.
5.2 It is the user’s responsibility to use safe and proper techniques when handling materials in this
test method. Consult manufacturers for specific details, such as material safety data sheets and other
recommendations.
5.3 Good laboratory practice should be followed. Wear safety glasses in all laboratory areas, and a dust
respirator and single-use gloves while handling powder colorants and aromatic amines.
5.4 Users should comply with any national and local safety regulations.
6 Apparatus
Usual laboratory equipment and, in
...