SIST EN 46-2:2016

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung (Laboratoriumsverfahren)Produits de préservation du bois - Détermination de l'action préventive contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide (Méthode de laboratoire)Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method)71.100.50Wood-protecting chemicalsICS:Ta slovenski standard je istoveten z:EN 46-2:2016SIST EN 46-2:2016en,fr,de01-september-2016SIST EN 46-2:2016SLOVENSKI
STANDARDSIST EN 46-2:20101DGRPHãþD



SIST EN 46-2:2016



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 46-2
June
t r s x ICS
y sä s r rä w r Supersedes EN
v xæ tã t r r {English Version
Wood preservatives æ Determination of the preventive action against recently hatched larvae of Hylotrupes Produits de préservation du bois æ Détermination de l 5action préventive contre les larves récemment écloses
Holzschutzmittel æ Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von This European Standard was approved by CEN on
w January
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey andUnited Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
9
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
v xæ tã t r s x ESIST EN 46-2:2016



EN 46-2:2016 (E) 2 Contents Page European foreword . 4 Introduction . 5 1 Scope . 6 2 Normative references . 6 3 Terms and definitions . 6 4 Principle . 7 5 Test materials . 7 5.1 Biological material . 7 5.2 Products and reagents . 7 5.3 Apparatus . 7 6 Sampling . 8 7 Test specimens . 8 7.1 Species of wood. 8 7.2 Wood quality . 8 7.3 Provision of test specimens . 9 7.4 Dimensions of test specimens . 9 7.5 Number of test specimens. 9 7.5.1 Test specimens for egg-laying . 9 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae . 10 8 Procedure . 10 8.1 Preparation of the test specimens . 10 8.1.1 Conditioning of the test specimens prior to sealing . 10 8.1.2 Sealing of block faces . 10 8.1.3 Treatment of the test specimens . 10 8.1.4 Drying and conditioning of the test specimens after treatment . 12 8.2 Exposure of the test specimens to the insects (ovicidal action) . 12 8.3 Validity of the test . 13 9 Expression of results . 13 9.1 Ovicidal test . 13 9.2 Tunnelling control . 13 9.3 Total mortality . 14 10 Test report . 14 Annex A (informative)
Example of a test report . 16 Annex B (informative)
Technique for culturing Hylotrupes bajulus (Linnaeus) . 18 B.1 General . 18 B.2 Obtaining parent beetles . 18 B.3 Mating . 18 SIST EN 46-2:2016



EN 46-2:2016 (E) 3 B.4 Egg-Iaying . 18 B.5 Hatching of eggs . 19 B.6 Larval development . 19 B.7 Enemies and parasites . 20 Annex C (informative)
Differentiation of heartwood and sapwood in Pinus species . 21 C.1 Principle . 21 C.2 Reagents . 21 C.3 Apparatus . 21 C.4 Procedure . 21 Annex D (informative)
Environmental, health and safety precautions within chemical/biological laboratory . 22 Bibliography . 23
SIST EN 46-2:2016



EN 46-2:2016 (E) 4 European foreword This document (EN 46-2:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicting national standards shall be withdrawn at the latest by December 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 46-2:2009. Significant technical differences between this document and EN 46-2:2009 are as follows: a)
introduction of new harmonised specifications for wood quality; b)
option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality. The standard EN 46 is composed of two parts: — EN 46-1, Wood preservatives
wã Application by surface treatment
— EN 46-2, Wood preservatives
EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidal effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. SIST EN 46-2:2016



EN 46-2:2016 (E) 5 Introduction This test method describes a laboratory method of test which gives a basis for the assessment of the preventive action of a wood preservative, when applied as a surface treatment for timber, against eggs of Hylotrupes bajulusä In combination with EN 46-1 it provides a means of checking whether larvae may hatch from eggs laid on the treated wood surface and whether they are capable of boring through the treated surface and of surviving in the untreated part of the wood. This standard provides information for the sealing of all but one lateral face when specimens are to be treated by dipping. When products which are very active at very low concentration are used, it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). SIST EN 46-2:2016



EN 46-2:2016 (E) 6 1 Scope This European Standard specifies a method for the determination of the preventive action of a wood preservative against eggs of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: — water-insoluble chemicals which are being studied as active insecticides; — organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; — organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or — water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 46-1, Wood preservatives — Determination of the preventive action against recently hatched —
EN 73, Wood preservatives — Accelerated ageing of treated wood prior to biological testing — Evaporative ageing procedure EN 84, Wood preservatives — Accelerated ageing of treated wood prior to biological testing — Leaching procedure EN ISO 3696, Water for analytical laboratory use —
3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [SOURCE: EN 1001-2:2005, 4.71] SIST EN 46-2:2016



EN 46-2:2016 (E) 7 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 1001–2:2005, 4.83. 4 Principle In this laboratory method treated wood panels are offered to freshly mated Hylotrupes bajulus females. The hatching ability of the larvae on the treated timber is examined. When the ovicidal action is insufficient, the mortality of the hatched larvae on and/or in wood treated with the same formulation is also established according to EN 46-1. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) females. 5.1.2 Source of females The insects shall preferably be obtained from cultures reared as e.g. described in Annex B. Use only sound and lively insects. 5.2 Products and reagents 5.2.1 Paraffin wax, for fixing the glass plate in all cases and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable. 5.2.2 Gelatine, for sealing the end faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION — Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at relative humidity (70 ± 5) %. 5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %. The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). SIST EN 46-2:2016



EN 46-2:2016 (E) 8 5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated and air-conditioned, controlled at (22 ± 2) °C and at relative humidity between (70 ± 5) %. 5.3.5 Petri dishes of glass or polyvinylchloride (PVC), diameter ca. 9 cm for mating the insects and for egg-laying. 5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.7 Safety equipment, protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Glass plates, (48 ± 1) mm long and (25 ± 1) mm wide, intended to provide a lateral slit on the test specimens. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g and equipment for applying a liquid product by brushing or by pipette. 5.3.10 Protective gloves 6 Sampling The sample of preservative shall be representative of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used. 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). Additional tests may be carried out using other species but, if so, this shall be stated in the test report. 7.2 Wood quality The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. The trees shall be cut immediately after felling
1) In southern European countries the pine species most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance of impregnation, etc.). SIST EN 46-2:2016



EN 46-2:2016 (E) 9 and the timber rapidly air dried or kiln dried at temperatures below 60 °C. The wood shall not have been stored for more than five years. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole. It is recommended to use test specimens of similar growth rate within a single test. 7.3 Provision of test specimens2) Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of 2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall have a contact angle of 45° ± 15° to the broad faces. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens (50 ± 0,5) mm long. The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock originally of more than 500 test specimens. 7.4 Dimensions of test specimens The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be (50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm. Mark each test specimen so that it can be identified throughout the test. 7.5 Number of test specimens 7.5.1 Test specimens for egg-laying a) Six treated test specimens (no more than two originating from the same tree unless taken at random from a stock of more than 500) for each preservative, each concentration and each duration of treatment; b) three untreated control test specimens (each originating from a different tree unless taken at random from a stock of more than 500) for a complete test of any given preservative; c) three control test specimens treated with the solvent or diluent (5.2.3 or 5.2.4) (each originating from a different tree unless taken at random from a stock of more than 500) if a solvent or diluent (including water) is used. Control test specimens under c) may be omitted if the solvent or diluents is water of drinking quality When dipping is to be used (8.1.3.3) it is advisable to treat more than the specified number of test specimens so that, after weighing, any test specimens with abnormally high or low retentions can be rejected from the batch. NOTE To gain further information on a formulation, the manufacturer may find it useful to test a version of the preservative where the active ingredient(s) has been removed.
2) For special tests, test specimens may be obtained according to a given series. As a result, it may be preferable to take test specimens from pretreated strips. Where pretreated strips are used details should be included in the test report. SIST EN 46-2:2016



EN 46-2:2016 (E) 10 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae In addition to the test specimens for egg-laying at least six test specimens shall be prepared for each preservative concentration and retention for checking the tunnelling ability and the mortality of the newly hatched larvae. 8 Procedure 8.1 Preparation of the test specimens 8.1.1 Conditioning of the test specimens prior to sealing Allow the test specimens to condition in the conditioning chamber (5.3.2) for a minimum of two weeks. 8.1.2 Sealing of block faces 8.1.2.1 General When treatment is to be by brushing or by pipette then only the transverse faces of test specimens shall be sealed. When treatment is to be by dipping then all faces, except one 25 mm x 50 mm face, shall be sealed. The material used for sealing shall be resistant to the penetration of wood preservatives under test. The sealings specified in 8.1.2.2 and 8.1.2.3 have been proven as suitable. 8.1.2.2 For tests with preservative solutions in which water is the continuous phase, apply three coats of the paraffin wax (5.2.1) at about 90 °C so that the first coat adheres closely to the wood and the successive coatings bond to one another. Condition the sealed test specimens in the conditioning chamber (5.3.2) for at least one day. 8.1.2.3 For tests with preservative solutions in which the continuous phase is an organic solvent that dissolves paraffin wax, use the gelatine (5.2.2): apply the first coat as an aqueous solution of 200 g/l at 40 °C, then after a minimum of 8 h of drying, apply two further coats of an aqueous solution of 300 g/l at 50 °C. Condition the sealed test specimens in the conditioning chamber (5.3.2) for at least one day. 8.1.3 Treatment of the test specimens 8.1.3.1 Preparation of the treatment solutions 8.1.3.1.1 Solid preservatives — Water-soluble preservatives: Dissolve the preservative in the water (5.2.3) to the concentration recommended by the manufacturer. — Non-water-soluble preservatives: Dissolve the preservative in an appropriate solvent (5.2.4) to the concentration recommended by the manufacturer. All treatment solutions shall be freshly prepared. SIST EN 46-2:2016



EN 46-2:2016 (E) 11 8.1.3.1.2 Liquid preservatives If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a concentrate, dilute the preservative with the diluent specified by the manufacturer to the required working concentration. A formulation of the preservative to be tested without active ingredients shall also be included in the test. All treatment solutions shall be freshly prepared. 8.1.3.2 Treatment by brushing or by pipette In the laboratory work area (5.3.3) apply the preservative either by brushing or by pipette to the 50 mm x 25 mm lateral face that would have been furthest from the centre of the tree. Depending on the type of treatment, the volume (application by pipette) or mass (application by brushing) of the treatment solution shall be determined to obtain the surface application specified by the manufacturer. Place the specimens such that the 50 mm x 25 mm lateral face that is to be treated is uppermost and apply the appropriate fluid uniformly to that face. When the preservative is applied by brush, place the test specimens on a balance while being brushed to determine the amount of preservative applied to the nearest 0,01 g. When the preservative is applied by pipette, move the pipette across the fibre direction and the amount of preservative applied shall be determined to the nearest 0,01 ml. Several applications can be necessary to apply the required amount. In this case the coats should be applied sufficiently quickly to avoid any solidification of certain substances which can impede the penetration of further coats. Care shall be taken to avoid fluid running off the lateral face being treated. 8.1.3.3 Treatment by dipping When the treatment of specimens is by dipping all faces, except one 25 mm x 50 mm face, shall be sealed (see 8.1.2). Weigh to the nearest 0,01 g any sealed test specimen, to obtain its initial mass. Immerse one test specimen after the other in the treatment solution, moving it during dipping. The dipping time to be used shall be one of the following, agreed beforehand according to the purpose of the test: — Either one 10 s period and/or two periods of 10 s at an interval of 24 h. If the rate of solidification of some constituents of a preservative formulation would have the effect of retarding its penetration during the second dipping, this interval has to be reduced. The interval employed shall be mentioned in the test report. SIST EN 46-2:2016



EN 46-2:2016 (E) 12 — Or a period sufficient for a determined quantity to be retained by the test specimen3). Using forceps, remove each test specimen from the preservative fluid and sponge off fluid from all the sealed faces of the specimen. Keeping the face that has not been sealed upper most, immediately weigh to the nearest 0,01 g. In the case of water-soluble chemicals, for example salts, and water-insoluble chemicals which are being studied as active ingredients, calculate the mass of chemical retained for each test specimen from the mass of solution absorbed and its concentration. In the case of organic formulations or organic water-dispersible formulations, the retention is expressed for each test specimen in terms of the corresponding mass of the formulation ready for use, but if a concentrate is supplied the retention is expressed in terms of the dilution applied. Calculate the mass of preservative retained in grams per square meter of timber surface. 8.1.4 Drying and conditioning of the test specimens after treatment If the end-sealing has been damaged before or after treatment, reject the test specimens concerned from the tests. After treatment, condition the test specimens for four weeks in the environment specified for the conditioning chamber (5.3.2). Arrange the test specimens on their lateral narrow faces, resting on glass rods, not touching one another. Invert the test specimens twice a week. NOTE The drying and conditioning of the test specimens depend on the nature of the product under test and on the solvent or diluent used. For slow drying products it may be necessary to extend the conditioning process. If, in the case of slow drying products, the conditioning period is extended, the extended conditioning period shall be stated in the test report. If the test specimens are to be subject to an ageing procedure (according to EN 73 or EN 84), this shall be carried out after this drying procedure. 8.2 Exposure of the test specimens to the insects (ovicidal action) Mate young Hylotrupes females in Petri dishes (5.3.5). After mating transfer the females singly to other Petri dishes (5.3.5) with centrally placed test specimens (7.5.1). Place the treated face of the test specimen on the bottom of the Petri dish leaving a gap for egg-laying. The test specimens are now ready for egg-laying by the house longhorn beetle. Egg-laying extends over seven days under the conditions of the testing chamber (5.3.4). After the first egg-laying remove the females. In case less than ten eggs have been deposited on a test specimen, repeat the egg-laying procedure with a freshly mated female. In case no eggs have been laid within one week, repeat the procedure with up to three freshly mated
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